荧光染料TMRM(Synonyms: 四甲基罗丹明甲酯) | Cytiva思拓凡-Whatman滤纸滤膜滤器

荧光染料Dye Reagents TMRM;(Synonyms: 四甲基罗丹明甲酯)

TMRM 是一种细胞渗透性阳离子亲脂性红色荧光染料 (λ_ex=530 nm, λ_em=592 nm)。

TMRM Chemical Structure

CAS No. : 115532-49-5

规格		是否有货
100 mg	;	询价	;
250 mg	;	询价	;
500 mg	;	询价	;

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TMRM 的其他形式现货产品:

TMRM Perchlorate

生物活性	TMRM is a cell-permeant cationic lipophilic red fluorescent dye (λ_ex=530 nm, λ_em=592 nm).
体外研究 (In Vitro)	TMRM is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of safranin or TMRM shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM (2 μM) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%^[1]. Exposure of hippocampal cultures to low concentrations of TMRM (50 to 500 nM) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM (1 to 25 µM) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 µM) enhance apoptosis (K_D = 500 nM)^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	401.48
Formula	C₂₅H₂₅N₂O₃⁺
CAS 号	115532-49-5
中文名称	四甲基罗丹明甲酯
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献	[1]. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286. [2]. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059.

Cell Assay ^[1]	Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM and/or drugs for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286. [2]. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059.

Cell Assay
^[1]

Cultures are exposed to Millipore-filtered solutions (0.22 µm) containing TMRM and/or drugs for 1 hr at 37°C (except the experiment involving different durations of exposure to TMRM). After treatment, solutions are removed and growth media reapplied under sterile conditions, and cultures are post-incubated for 18 hours at 37°C (except for the experiment involving analysis at different time points after exposure). Cells are then stained with 2 mg/mL bisbenzimide for 20 min at room temperature. Coverslips are subsequently washed in saline and imaged using 2P microscopy. Apoptotic cells are identified as brightly fluorescent nuclei under UV excitation indicating DNA fragmentation. Cell survivability is calculated as the percentage of live, unstained cells (±SD) in five microscopic fields per treatment^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Chowdhury SR, et al. Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep. 2015 Dec 8;36(1):e00286.

[2]. Monteith A, et al. Imaging of mitochondrial and non-mitochondrial responses in cultured rat hippocampal neurons exposed to micromolar concentrations of TMRM. PLoS One. 2013;8(3):e58059.