Ginsenoside Rb2 is one of the main bioactive components of ginseng extracts. Rb2 can upregulate GPR120 gene expression. Ginsenoside Rb2 has antiviral effects.

Ginsenoside Rb2 pre-treatment enhances the anti-inflammatory effect of α-linolenic acid (ALA) and that the enhancing effect is strictly dependent on GPR120 activation. Ginsenoside Rb2 exerts anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW264.7 cells in vitro by increasing GPR120 expression and subsequently enhancing ω-3 fatty acid-induced GPR120 activation. Ginsenoside Rb2 improves glucose metabolism in hepatocytes by activating AMPK and reduces cholesterol and triacylglycerol levels in 3T3-L1 cells by reducing oxidative damage. Ginsenoside Rb2 exerts anti-apoptosis effects in murine bone marrow-derived mesenchymal stem cells (BMMSCs). MTT assay results show no obvious cytotoxicity of Ginsenoside Rb2 (up to 100 μM) toward RAW264.7 cells in the absence or presence of ALA. The influence of Rb2 on GPR120 expression in RAW264.7 macrophages is investigated by treating the cells with Ginsenoside Rb2 (0.1-100 μM) for 12 h followed by harvesting and lysis. Subsequent Western blot analysis shows that expression of GPR120 is dose-dependently upregulated by Ginsenoside Rb2. Real-time PCR results indicate that incubation of RAW264.7 macrophages with Ginsenoside Rb2 (10 μM) for 12 h leads to a 2.8-fold increase in GPR120 mRNA expression. In addition, this increase in GPR120 expression stimulated by Ginsenoside Rb2 is time dependent and begins as early as 6 h. These results indicate that Rb2 upregulates GPR120 expression in a dose- and time-dependent manner in RAW264.7 macrophages^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Ginsenoside Rb2 is an antiviral reagent to protect against rotavirus (RV) infection. When various dosages of Ginsenoside Rb2 (25 to 250 mg/kg) are administered 3, 2 or 1 days before virus challenge, treatment with this Ginsenoside at the dosage of 75 mg/kg 3 days before virus infection most effectively reduces rotavirus (RV) -induced diarrhea. In addition, consecutive administration of Ginsenoside Rb2 (75 mg/kg) 3, 2, and 1 day before virus infection is more effective than single administration on day-3. The consecutive administration of Ginsenoside Rb2 also reduces virus titers in the bowels of RV-infected mice^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1079.27

C₅₃H₉₀O₂₂

11021-13-9

人参皂苷 Rb2

Room temperature in continental US; may vary elsewhere.

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

In Vitro:;

H₂O : ≥ 100 mg/mL (92.66 mM)

DMSO : 9.5 mg/mL (8.80 mM; Need ultrasonic and warming)

* “≥” means soluble, but saturation unknown.

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: ≥ 0.92 mg/mL (0.85 mM); Clear solution

  此方案可获得 ≥ 0.92 mg/mL (0.85 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 9.2 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 0.92 mg/mL (0.85 mM); Clear solution

  此方案可获得 ≥ 0.92 mg/mL (0.85 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 9.2 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
• 3.

  请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

  Solubility: ≥ 0.92 mg/mL (0.85 mM); Clear solution

  此方案可获得 ≥ 0.92 mg/mL (0.85 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

  以 1 mL 工作液为例，取 100 μL 9.2 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

• [1]. Huang Q, et al. Ginsenoside Rb2 enhances the anti-inflammatory effect of ω-3 fatty acid in LPS-stimulated RAW264.7 macrophages by upregulating GPR120 expression. Acta Pharmacol Sin. 2017 Feb;38(2):192-200.

  [2]. Yang H, et al. Ginsenoside-Rb2and 20(S)-Ginsenoside-Rg3 from Koreanred ginseng prevent rotavirus infection in newborn mice. J Microbiol Biotechnol. 2018 Jan 11.

Cell viability is determined using the MTT assay. RAW264.7 cells (2×10⁴ cells/well) are plated in 96-well plates and cultured overnight in growth medium. The cells are then incubated with Ginsenoside Rb2 (0.1, 1, 10 and 100 μM) at the indicated concentrations in the absence or presence of ALA for 48 h before addition of the MTT reagent (0.5 mg/mL). After incubation for 4 h, the medium is removed, and the formazan crystals formed are dissolved with 100 μL DMSO. Absorbance at a wavelength of 492 nm is measured using a FlexStation 3^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Mice^[2]
Groups of five BALB/c newborn mice are inoculated p.o. with RV-SA11 (1.5×10⁶ plaque-forming units (PFU)/mouse) and administered p.o. with the indicated doses of Ginsenoside Rb2 on the indicated days. In order to examine the protective effect of Ginsenoside Rb2 on RV infection, newborn mice are treated orally with 75 mg/kg of this Ginsenoside 3, 2 or 1 day before RV infection, and the severity of diarrhea of RV-infected mice i calculated. In addition, in an experiment in which various doses of Ginsenoside Rb2 ranging from 25 to 250 mg/kg are administered to mice 3 days before RV infection, Ginsenoside Rb2 at the dose of 75 mg/kg elicits higher protective activity compared to either of the dose of 25 to 250 mg/kg.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Huang Q, et al. Ginsenoside Rb2 enhances the anti-inflammatory effect of ω-3 fatty acid in LPS-stimulated RAW264.7 macrophages by upregulating GPR120 expression. Acta Pharmacol Sin. 2017 Feb;38(2):192-200.

  [2]. Yang H, et al. Ginsenoside-Rb2and 20(S)-Ginsenoside-Rg3 from Koreanred ginseng prevent rotavirus infection in newborn mice. J Microbiol Biotechnol. 2018 Jan 11.