Cirsilineol(Synonyms: 甲基条叶蓟素)

发表于由

天然产物 黄酮类 Flavonoids

Cirsilineol (Synonyms: 甲基条叶蓟素) 纯度: ≥98.0%

Cirsilineol 是一种天然黄酮化合物，可选择性抑制肠道 CD4+ T 细胞中的 IFN-γ/STAT1/T-bet 信号。Cirsilineol 具有免疫抑制和抗肿瘤特性。Cirsilineol 显着改善三硝基苯磺酸 (TNBS) 诱导的小鼠 T 细胞介导的实验性结肠炎。

Cirsilineol(Synonyms: 甲基条叶蓟素)

Cirsilineol Chemical Structure

CAS No. : 41365-32-6

规格 价格 是否有货 数量
1 mg ￥4200 In-stock
5 mg ￥14800 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

生物活性

Cirsilineol, a natural flavone compound, selectively inhibits IFN-γ/STAT1/T-bet signaling in intestinal CD4+ T cells. Cirsilineol has potent immunosuppressive and anti-tumor properties. Cirsilineol significantly ameliorates trinitro-benzene sulfonic acid (TNBS)-induced T-cell-mediated experimental colitis in mice[1].

体外研究
(In Vitro)

Cirsilineol (0.1-10 μM; 96 hours) inhibits single mixed lymphocyte reaction and Concanava A-induced T-cell proliferation in a dose-dependent manner. Cirsilineol (10 μM) does not affect T lymphocyte’s viability. The inhibition of cirsilineol (10 μM) on T-cell proliferation is not due to a cytotoxic activity[1].
Cirsilineol (1-10 μM; pretreatment for 3 hours) completely inhibits IFN-γ-induced Tyr701 phosphorylation of STAT1 and JAK2 activation[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[1]

Cell Line: Splenocytes
Concentration: 0.1, 1, 10 μM
Incubation Time: 96 hours
Result: Inhibited single mixed lymphocyte reaction and Concanavalin A (Con A; 5 μg/mL; for 72 h)-induced T-cell proliferation in a dose-dependent manner.
Suppressed OVA323-339-specific CD4+ T-cell proliferation.

Western Blot Analysis[1]

Cell Line: Splenic CD4+ T cells
Concentration: 1, 5, 10 μM
Incubation Time: Pretreatment for 3 hours
Result: Completely inhibited IFN-γ-induced (25 ng; 30 mins) Tyr701 phosphorylation of STAT1 and JAK2 activation.
Suppressed IFN-γ-induced (25 ng; 12 hours) Th1-specific transcription factor T-bet.

体内研究
(In Vivo)

cirsilineol (3, 10, and 30 mg/kg) significantly ameliorates TNBS-induced Th1-mediated colitis through inhibiting IFN-γ/STAT1/T-bet signaling in CD4+ T cells.

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 8-10-week-old female C57BL/6, BALB/c and DO11.10 transgenic mice with TNBS (10 mg; 100 μL)[1]
Dosage: 3, 10, 30 mg/kg
Administration: IP; daily; for 11 days
Result: Showed a significant improved effect on the body weights and survival rate of mice.
Markedly reduced inflammatory infiltration, restoration of the destructive mucosal architecture and remission of edema.

分子量

344.32

Formula

C18H16O7
CAS 号

41365-32-6
中文名称

甲基条叶蓟素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
参考文献

  • [1]. Yang Sun, et al. Novel immunomodulatory properties of cirsilineol through selective inhibition of IFN-gamma signaling in a murine model of inflammatory bowel disease. Biochem Pharmacol. 2010 Jan 15;79(2):229-38.