分类目录归档：黄酮类

天然产物黄酮类

Kaempferol 4′-glucoside(Synonyms: Kaempferol 4′-β-glucoside)

发表于由

天然产物 黄酮类 Flavonoids

Kaempferol 4′-glucoside (Synonyms: Kaempferol 4′-β-glucoside)

Kaempferol 4′-glucoside (Kaempferol 4′-β-glucoside) 是一种黄酮醇苷。

Kaempferol 4

Kaempferol 4′-glucoside Chemical Structure

CAS No. : 52222-74-9

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生物活性

Kaempferol 4′-glucoside (Kaempferol 4′-β-glucoside) is a flavonol glycoside.

分子量

448.38

Formula

C21H20O11
CAS 号

52222-74-9
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Yan Zheng, et al. Effect of pH on the complexation of kaempferol-4′-glucoside with three β-cyclodextrin derivatives: isothermal titration calorimetry and spectroscopy study. J Agric Food Chem. 2014 Jan 8;62(1):244-50.

(R)-Eucomol

发表于由

天然产物 黄酮类 Flavonoids

(R)-Eucomol 

(R)-Eucomol 是一种类黄酮衍生物，显示出微弱的抗菌活性。(R)-Eucomol 对 KB 和 P-388 细胞显示出细胞毒活性。

(R)-Eucomol

(R)-Eucomol Chemical Structure

CAS No. : 118204-64-1

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生物活性

(R)-Eucomol, a flavonoid derivative, displays marginal antibacterial activity. (R)-Eucomol shows cytotoxic activity against KB and P-388 cells[1].

分子量

316.31

Formula

C17H16O6
CAS 号

118204-64-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. TRADITIONAL MEDICINAL PLANTS OF THAILAND XIII. FLAVONOID DERIVATIVES FROM DRACAENA LOUREIRI ( AGAVACEAE)*

6-Formyl-isoophiopogonanone A(Synonyms: 6-醛基异麦冬黄烷酮A)

发表于由

天然产物 黄酮类 Flavonoids

6-Formyl-isoophiopogonanone A (Synonyms: 6-醛基异麦冬黄烷酮A)

6-Formyl-isoophiopogonanone A 是从 Ophiopogon japonicas 中提取的 homoisoflavonoidal，具有抗氧化活性。

6-Formyl-isoophiopogonanone A(Synonyms: 6-醛基异麦冬黄烷酮A)

6-Formyl-isoophiopogonanone A Chemical Structure

CAS No. : 116291-82-8

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1 mg ￥1300 询问价格 & 货期
5 mg ￥3200 询问价格 & 货期

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生物活性

6-Formyl-isoophiopogonanone A is a homoisoflavonoidal extracted from Ophiopogon japonicas, with antioxidant activity[1].

分子量

356.33

Formula

C19H16O7
CAS 号

116291-82-8
中文名称

6-醛基异麦冬黄烷酮A
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Ma C, et al. An efficient combination of supercritical fluid extraction and high-speed counter-current chromatography to extract and purify homoisoflavonoids from Ophiopogon japonicus (Thunb.) Ker-Gawler. J Sep Sci. 2009 Jun;32(11):1949-56.

Polyporusterone A(Synonyms: 猪苓酮A)

发表于由

天然产物 黄酮类 Flavonoids

Polyporusterone A (Synonyms: 猪苓酮A)

Polyporusterone A 是一种从 Polyporus umbellatus Fries 中分离出来的三萜羧酸。Polyporusterone A 对自由基诱导的红细胞裂解 (溶血) 具有抑制作用。

Polyporusterone A(Synonyms: 猪苓酮A)

Polyporusterone A Chemical Structure

CAS No. : 141360-88-5

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1 mg ￥1400 询问价格 & 货期

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生物活性

Polyporusterone A is a triterpene carboxylic acid isolated from Polyporus umbellatus Fries. Polyporusterone A has inhibitory effect on free radical-induced lysis of red blood cells (hemolysis)[1].

分子量

478.66

Formula

C28H46O6
CAS 号

141360-88-5
中文名称

猪苓酮A
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Nobuyasu Sekiya, et al. Inhibitory effects of triterpenes isolated from Chuling (Polyporus umbellatus Fries) on free radical-induced lysis of red blood cells. Biol Pharm Bull. 2005 May;28(5):817-21.

Kushenol L(Synonyms: 苦参醇L)

发表于由

天然产物 黄酮类 Flavonoids

Kushenol L (Synonyms: 苦参醇L)

Kushenol L 是苦参乙醇提取物的主要成分之一，苦参是来自 Sophora flavescens Ait 的干燥根。Kushenol L 是一种类黄酮，具有抗糖尿病的作用。

Kushenol L(Synonyms: 苦参醇L)

Kushenol L Chemical Structure

CAS No. : 101236-50-4

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生物活性

Kushenol L is one of the main components of EtOA cextracts from Kushen, a traditional medicinal herb derived from the dried roots of Sophora flavescens Ait. Kushenol L is a flavonoid and plays an important role in anti-diabeticeffects[1].

体内研究
(In Vivo)

Kushenol L (1 mg/kg; oral) has a T1/2 of 2.26 hours, a Cmax of 24.17 μg/L and an AUC of 54035 μg/h•L in adult male Sprague-Dawley rats weighing 300 g[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

440.49

Formula

C25H28O7
CAS 号

101236-50-4
中文名称

苦参醇L
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Lei Chen, et al. An accurate and reproducible method for simultaneous determination of four flavonoids in EtOAc extracts from Sophora flavescens Ait. in rat plasma based on UHPLC Q-Exactive Mass spectrometry: Application to a pharmacokinetics study. Biomed Chromatogr. 2019 Mar;33(3):e4447.

Kushenol N(Synonyms: 苦参醇N)

发表于由

天然产物 黄酮类 Flavonoids

Kushenol N (Synonyms: 苦参醇N)

Kushenol N 是一种异戊烯基黄酮类化合物，能从 Sophora flavescens 中分离得到。Kushenol N 具有抗过敏和血管舒张作用。

Kushenol N(Synonyms: 苦参醇N)

Kushenol N Chemical Structure

CAS No. : 102490-65-3

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生物活性

Kushenol N is a prenylated flavonoid that can be isolated from the root of Sophora flavescens. Kushenol N has anti-allergic and vasorelaxation activities[1][2].

分子量

454.51

Formula

C26H30O7
CAS 号

102490-65-3
中文名称

苦参醇N
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Wenying Quan, et al. Anti-allergic prenylated flavonoids from the roots of Sophora flavescens. Planta Med. 2008 Feb;74(2):168-70.

    [2]. Chul Young Kim, et al. Vasorelaxant prenylated flavonoids from the roots of Sophora flavescens. Biosci Biotechnol Biochem. 2013;77(2):395-7.

Quercetin(Synonyms: 槲皮素)

发表于由

天然产物 黄酮类 Flavonoids

Quercetin (Synonyms: 槲皮素) 纯度: 98.02%

Quercetin 是一种天然黄酮类化合物，可激活或抑制许多蛋白质的活性。Quercetin 可激活 SIRT1，也可抑制 PI3K，抑制 PI3Kγ，PI3Kδ，PI3Kβ 的 IC50 分别为 2.4 μM, 3.0 μM, 5.4 μM。

Quercetin(Synonyms: 槲皮素)

Quercetin Chemical Structure

CAS No. : 117-39-5

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10 mM * 1 mL in DMSO ￥500 In-stock
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1 g ￥500 In-stock
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生物活性

Quercetin, a natural flavonoid, is a stimulator of recombinant SIRT1 and also a PI3K inhibitor with IC50 of 2.4 μM, 3.0 μM and 5.4 μM for PI3K γ, PI3K δ and PI3K β, respectively[1].

IC50 & Target[1]

PI3Kδ

2.4 μM (IC50)

PI3Kγ

3 μM (IC50)

PI3Kβ

5.4 μM (IC50)

Autophagy

 

Mitophagy

 

体外研究
(In Vitro)

Quercetin is a type of plant-based chemical, or phytochemical, used as an ingredient in supplements, beverages or foods. In several studies, it may have anti-inflammatory and antioxidant properties, and it is being investigated for a wide range of potential health benefits. Quercetin is a PI3K inhibitor with IC50 of 2.4-5.4 μM. Quercetin strongly abrogates PI3K and Src kinases, mildly inhibits Akt1/2, and slightly affected PKC, p38 and ERK1/2[1]. Quercetin inhibits TNF-induced LDH% release, EC-dependent neutrophils adhesion to bovine pulmonary artery endothelial cells (BPAEC), and BPAEC DNA synthesis and proliferation[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Combination of Quercetin (75 mg/kg) and 2-Methoxyestradiol enhances inhibition of human prostate cancer LNCaP and PC-3 cells xenograft tumor growth[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

302.24

Formula

C15H10O7
CAS 号

117-39-5
中文名称

槲皮素；栎精；槲皮黄素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 100 mg/mL (330.86 mM)

Ethanol : 16.67 mg/mL (55.15 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3086 mL 16.5431 mL 33.0863 mL
5 mM 0.6617 mL 3.3086 mL 6.6173 mL
10 mM 0.3309 mL 1.6543 mL 3.3086 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 0.5% CMC-Na/saline water

    Solubility: 25 mg/mL (82.72 mM); Suspended solution; Need ultrasonic

  • 2.

    请依序添加每种溶剂： 45% PEG300    5% Tween-80    50% saline

    Solubility: 10 mg/mL (33.09 mM); Suspended solution; Need ultrasonic

  • 3.

    请依序添加每种溶剂： 50% PG  50% Saline

    Solubility: 10 mg/mL (33.09 mM); Suspended solution; Need ultrasonic

  • 4.

    请依序添加每种溶剂： 50%PG  50%Saline

    Solubility: 10 mg/mL (33.09 mM); Suspended solution; Need ultrasonic

  • 5.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.27 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 6.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.27 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Leyre Navarro-Núñez, et al. Effect of quercetin on platelet spreading on collagen and fibrinogen and on multiple platelet kinases. Fitoterapia. 2010 Mar;81(2):75-80.

    [2]. Yu XB, et al. Inhibitory effects of protein kinase C inhibitors on tumor necrosis factor induced bovine pulmonary artery endothelial cell injuries. Yao Xue Xue Bao. 1996;31(3):176-81.

    [3]. Yang F, et al. Combination of Quercetin and 2-Methoxyestradiol Enhances Inhibition of Human Prostate Cancer LNCaP and PC-3 Cells Xenograft Tumor Growth. PLoS One. 2015 May 26;10(5):e0128277.

    [4]. Tao Liu, et al. Quercetin alleviates kidney fibrosis by reducing renal tubular epithelial cell senescence through the SIRT1/PINK1/mitophagy axis. Life Sci. 2020 Jul 20;118116.
Animal Administration
[3]

Mice are inoculated subcutaneously with 5×105 PC-3 cells suspended in 100μL PBS and 2×108 LNCaP cells suspended in 100μL of matrigel and PBS mixture (1:1) on the right back. When xenograft tumors reach a volume of approximately 100 mm3, mice are randomLy assigned to four groups (n=8 each group) and treated intraperitoneally. Therapeutic schedule based on our in vitro results, preliminary experiments and many other researchers’ studies is as follows: (1) Vehicle control group: vehicle of quercetin on day 1, vehicle of 2-ME on day 2, (2) Quercetin treated group: quercetin 75 mg/kg on day 1, vehicle of 2-ME on day 2, (3) 2-ME treated group: vehicle of quercetin on day 1, 2-ME 150 mg/kg on day 2, (4) Combination treatment group: quercetin 75 mg/kg on day 1, 2-ME 150 mg/kg on day 2. Two days is a treatment cycle and the whole treatment process lasted for 4 weeks. Tumor sizes are monitored every 2 days using caliper and tumor volume are calculated according to the formula: L×S2×0.5, in which L represents the longest diameter and S represents the shortest diameter of tumor. Mice are weighed as well. At the end of treatment procedure, on day 29, mice are anesthetized with chloral hydrate and sacrificed by cervical dislocation. Xenograft tumors are taken out quickly and weighed. One part of it is put into liquid nitrogen immediately for future biomarker analysis and the other part is fixed in 10% neutral buffered formalin for immunohistochemical analysis. Serum biochemical parameters such as ALT, AST, creatinine and urea nitrogen that reflected drug toxicity are also detected.

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Leyre Navarro-Núñez, et al. Effect of quercetin on platelet spreading on collagen and fibrinogen and on multiple platelet kinases. Fitoterapia. 2010 Mar;81(2):75-80.

    [2]. Yu XB, et al. Inhibitory effects of protein kinase C inhibitors on tumor necrosis factor induced bovine pulmonary artery endothelial cell injuries. Yao Xue Xue Bao. 1996;31(3):176-81.

    [3]. Yang F, et al. Combination of Quercetin and 2-Methoxyestradiol Enhances Inhibition of Human Prostate Cancer LNCaP and PC-3 Cells Xenograft Tumor Growth. PLoS One. 2015 May 26;10(5):e0128277.

    [4]. Tao Liu, et al. Quercetin alleviates kidney fibrosis by reducing renal tubular epithelial cell senescence through the SIRT1/PINK1/mitophagy axis. Life Sci. 2020 Jul 20;118116.

5,7,4′-Trihydroxy-8-Methylflavanone(Synonyms: 8-Methyl-naringenine)

发表于由

天然产物 黄酮类 Flavonoids

5,7,4′-Trihydroxy-8-Methylflavanone (Synonyms: 8-Methyl-naringenine)

5,7,4′-Trihydroxy-8-Methylflavanone 是从 Qualea grandiflora 中分离得到的黄烷酮类化合物。

5,7,4

5,7,4′-Trihydroxy-8-Methylflavanone Chemical Structure

CAS No. : 916917-28-7

规格 价格 是否有货
1 mg ￥2860 询问价格 & 货期
5 mg ￥8570 询问价格 & 货期

* Please select Quantity before adding items.

生物活性

5,7,4′-Trihydroxy-8-Methylflavanone (Compound 2) is a flavanone isolated from Qualea grandiflora[1].

分子量

286.28

Formula

C16H14O5
CAS 号

916917-28-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Fausto Carnevale Neto, et al. Dereplication of phenolic derivatives of Qualea grandiflora and Qualea cordata (Vochysiaceae) using liquid chromatography coupled with ESI-QToF-MS/MS. J. Braz. Chem. Soc. May 2013, 24 (5).

(-)-Epigallocatechin Gallate(Synonyms: EGCG; Epigallocatechol Gallate)

发表于由

天然产物 黄酮类 Flavonoids

(-)-Epigallocatechin Gallate (Synonyms: EGCG; Epigallocatechol Gallate) 纯度: 99.87%

(-)-Epigallocatechin Gallate是一种茶类黄酮，具有强大的抗氧化，抗炎和抗癌作用。报道显示(-)-Epigallocatechin Gallate可抑制 EGFR 信号传导，从而发挥抗癌作用。(-)-Epigallocatechin Gallate (EGCG) 是谷氨酸脱氢酶 1/2 (GDH1/2，GLUD1/2) 抑制剂。(-)-Epigallocatechin Gallate 通过激活细胞色素c氧化酶来诱导氧化磷酸化 (OXPHOS)。

(-)-Epigallocatechin Gallate(Synonyms: EGCG; Epigallocatechol Gallate)

(-)-Epigallocatechin Gallate Chemical Structure

CAS No. : 989-51-5

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Free Sample (0.1-0.5 mg)   Apply now  
10 mM * 1 mL in DMSO ￥500 In-stock
50 mg ￥369 In-stock
100 mg ￥550 In-stock
500 mg ￥2100 In-stock
1 g   询价  
5 g   询价  

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生物活性

(-)-Epigallocatechin Gallate is a tea flavonoid with potent antioxidant, antiinflammatory, and anticarcinogenic properties. (-)-Epigallocatechin Gallate is reported to inhibit EGFR signaling and thereby exert anticancer effects[1]. (-)-Epigallocatechin Gallate (EGCG) is a glutamate dehydrogenase 1/2 (GDH1/2, GLUD1/2) inhibitor. Epigallocatechin-3-gallate induces oxidative phosphorylation (OXPHOS) by activating cytochrome c oxidase.

IC50 & Target[1]

EGFR

 

HER2

 

HER3

 

Human Endogenous Metabolite

 

体外研究
(In Vitro)

(-)-Epigallocatechin Gallate (EGCG) inhibits activation EGFR, HER2 and HER3 in the SW837 human colon cancer cell line. (-)-Epigallocatechin Gallate (10 μM) also inhibits cell growth, suppresses activation of EGFR, HER2, and HER3, and causes decrease in the levels of COX-2 and Bcl-xL proteins, and apoptosis after treatment for 96 h[1]. (-)-Epigallocatechin Gallate (0-35 μg/mL) inhibits the proliferation of colorectal cancer cells. (-)-Epigallocatechin Gallate (35 μg/mL) induces apoptosis of colorectal cancer cells[3]. (-)-Epigallocatechin Gallate (EGCG; 50, 75 and 100 μM) dose-dependently inhibits the growth of HepG2 cells, and induces apoptosis in HepG2 cells[4].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

(-)-Epigallocatechin Gallate (5, 10, and 20 mg/kg, p.o.) inhibits the growth of orthotopic colorectal cancer cells in mice[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

458.37

Formula

C22H18O11
CAS 号

989-51-5
中文名称

(-)-表没食子儿茶素没食子酸酯
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 30 mg/mL (65.45 mM)

H2O : 20 mg/mL (43.63 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.1816 mL 10.9082 mL 21.8164 mL
5 mM 0.4363 mL 2.1816 mL 4.3633 mL
10 mM 0.2182 mL 1.0908 mL 2.1816 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.54 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.54 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.54 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.54 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. De Amicis F, et al. Epigallocatechin gallate inhibits growth and Epithelial-to-Mesenchymal Transition in human thyroid carcinoma cell lines. J Cell Physiol. 2013 Apr 1.

    [2]. Peeters TH, et al. Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate. Cancer Metab. 2019 May 20;7:4.

    [3]. Jin H, et al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53.

    [4]. Wing Pui Tsang, et al. Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells. The Journal of Nutritional Biochemistry. 2010, 21(2): 140-146.

    [5]. Castellano-González G, et al. Epigallocatechin-3-gallate induces oxidative phosphorylation by activating cytochrome c oxidase in human cultured neurons and astrocytes. Oncotarget. 2016 Feb 16;7(7):7426-40.
Cell Assay
[3]

LoVo, SW480, HCT-8, and HT-29 cells are seeded in 96-well plates at a concentration of 5×103 cells; each cell line is totally seeded in the 12 wells. Complete medium is added to the wells, up to 200 μL; the medium contains 0 μg/mL, 10 μg/mL, 20 μg/mL, and 35 μg/mL of (-)-Epigallocatechin Gallate. The inhibition rate=[1 – (absorbance of (-)-Epigallocatechin Gallate group – absorbance of control group)/(absorbance of control group – absorbance of blank control group)] × 100[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[3]

Mice[3]
At 2 weeks postsurgery, 39 out of the 40 nude mice presented with tumors. Based on the volume of the tumors, the 39 mice with tumors are divided into four groups: a control group (n=9); a group that receives 5 mg/kg of (-)-Epigallocatechin Gallate (n=10); a group that receives 10 mg/kg of (-)-Epigallocatechin Gallate (n=10); and a group that receives 20 mg/kg of (-)-Epigallocatechin Gallate (n=10). In the therapeutic groups, (-)-Epigallocatechin Gallate is administrated intragastrically, and in the control group, 100 uL of physiological saline is administrated intragastrically, once daily for 14 days. After the treatment of the mice with (-)-Epigallocatechin Gallate for 4 weeks, the growth and metastasis of the primary tumors are continuously monitored using a fluorescent imaging system. After 4 weeks, the primary tumors are weighed and immediately put into liquid nitrogen (−196°C) and 2 to 3 hours later, these specimens are stored at −80°C. In addition, the other parts of the primary tumor and metastases are fixed in 4% formaldehyde[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. De Amicis F, et al. Epigallocatechin gallate inhibits growth and Epithelial-to-Mesenchymal Transition in human thyroid carcinoma cell lines. J Cell Physiol. 2013 Apr 1.

    [2]. Peeters TH, et al. Isocitrate dehydrogenase 1-mutated cancers are sensitive to the green tea polyphenol epigallocatechin-3-gallate. Cancer Metab. 2019 May 20;7:4.

    [3]. Jin H, et al. Epigallocatechin gallate inhibits the proliferation of colorectal cancer cells by regulating Notch signaling. Onco Targets Ther. 2013;6:145-53.

    [4]. Wing Pui Tsang, et al. Epigallocatechin gallate up-regulation of miR-16 and induction of apoptosis in human cancer cells. The Journal of Nutritional Biochemistry. 2010, 21(2): 140-146.

    [5]. Castellano-González G, et al. Epigallocatechin-3-gallate induces oxidative phosphorylation by activating cytochrome c oxidase in human cultured neurons and astrocytes. Oncotarget. 2016 Feb 16;7(7):7426-40.

Kaempferol 3,7,4′-trimethyl ether(Synonyms: 莰非醇三甲醚)

发表于由

天然产物 黄酮类 Flavonoids

Kaempferol 3,7,4′-trimethyl ether (Synonyms: 莰非醇三甲醚)

Kaempferol 3,7,4′-trimethyl ether 是一种从 Siparuna gigantotepala 中分离的黄酮醇苷元，具有抗氧化活性。

Kaempferol 3,7,4

Kaempferol 3,7,4′-trimethyl ether Chemical Structure

CAS No. : 15486-34-7

规格 价格 是否有货
1 mg ￥1500 询问价格 & 货期
5 mg ￥3750 询问价格 & 货期
10 mg ￥6350 询问价格 & 货期

* Please select Quantity before adding items.

生物活性

Kaempferol 3,7,4′-trimethyl ether is a flavonol aglycone isolated from the leaves of Siparuna gigantotepala, has antioxidant activity[1].

分子量

328.32

Formula

C18H16O6
CAS 号

15486-34-7
中文名称

莰非醇三甲醚
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Torres Castañeda HG, et al. Flavonoid Glycosides from Siparuna gigantotepala Leaves and Their Antioxidant Activity. Chem Pharm Bull (Tokyo). 2016;64(5):502-6.