FLAG peptide

FLAG peptide; 纯度: 99.21%

FLAG peptide是一种八个氨基酸组成的多肽 (Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys),具有肠激酶切割位点。FLAG peptide用于抗体介导的重组蛋白的识别和纯化。

FLAG peptideamp;;

FLAG peptide Chemical Structure

CAS No. : 98849-88-8

规格 价格 是否有货 数量
1 mg ¥500 In-stock
5 mg ¥900 In-stock
10 mg ¥1300 In-stock
25 mg ¥2390 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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FLAG peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library

生物活性

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

体外研究
(In Vitro)

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca2+. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure[1]. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca2+ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1012.97

Formula

C41H60N10O20

CAS 号

98849-88-8

Sequence

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Sequence Shortening

DYKDDDDK

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light, stored under nitrogen

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

H2O : 100 mg/mL (98.72 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9872 mL 4.9360 mL 9.8720 mL
5 mM 0.1974 mL 0.9872 mL 1.9744 mL
10 mM 0.0987 mL 0.4936 mL 0.9872 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Einhauer A, et al. The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Methods. 2001 Oct 30;49(1-3):455-65.

    [2]. Schuster M, et al. Protein expression in yeast; comparison of two expression strategies regarding proteinmaturation. J Biotechnol. 2000 Dec 28;84(3):237-48.

HA Peptide

HA Peptide; 纯度: 99.42%

HA Peptide (HA tag) 是来自人类流感血凝素 (HA) 的由九个氨基酸组成的多肽。HA Peptide 广泛用于细胞生物学和生物化学中分离,纯化,检测和追踪目标蛋白。

HA Peptideamp;;

HA Peptide Chemical Structure

CAS No. : 92000-76-5

规格 价格 是否有货 数量
1 mg ¥500 In-stock
5 mg ¥990 In-stock
10 mg ¥1650 In-stock
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HA Peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

HA Peptide (HA tag) is a nine amino acids peptide derived from the human influenza hemagglutinin (HA). HA Peptide is extensively used to isolate, purify, detect, and track the protein of interest in cell biology and biochemistry.

体外研究
(In Vitro)

HA Peptide is a highly immunoreactive tag generally used for the separation of tagged proteins from cell culture supernatants and cell lysate under neutral pH conditions and thus are handy tools for coimmunoprecipitation but are also easily detected via western blot. HA Peptide is small and thus unlikely to interfere with the bioactivity and function of the fusion partner proteins. HA Peptide comes from human influenza hemagglutinin (HA) corresponding to amino acids 98–106 and is a strong immunoreactive epitope making it popular to isolate, purify, detect, and track the protein of interest. The recombinant HA-tagged proteins can be separated by highly specific anti-HA monoclonal antibody that is covalently immobilized on resin. The HA-tagged proteins can be eluted by mild elution approach with HA epitope at 1 mg/mL in TBS. On the other hand, three chemical elution options are available: 0.1 M glycine (pH 2-2.8), 3 M NaSCN, or 50 mM NaOH[1]. The nucleotide sequences encoding an N-terminal HA Peptide in the mammalian expression vectors is an essential element for the T7 promoter-driven expression in E. coli even without trans-acting T7 RNAP[2]. Research results suggest that HA Peptide is cleaved by caspase 3/7, and HA Peptide cleavage results in a total loss of immunoreactivity. Observations indicate that the use of HA to tag proteins and constructs to study cell death–related and apoptotic mechanisms can result in serious artifacts[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1102.15

Formula

D2P2VY3

CAS 号

92000-76-5

Sequence Shortening

YPYDVPDYA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (45.37 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9073 mL 4.5366 mL 9.0732 mL
5 mM 0.1815 mL 0.9073 mL 1.8146 mL
10 mM 0.0907 mL 0.4537 mL 0.9073 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Zhao X, et al. Several affinity tags commonly used in chromatographic purification. J Anal Methods Chem. 2013;2013:581093.

    [2]. Moon JM, et al. A new idea for simple and rapid monitoring of gene expression: requirement of nucleotide sequences encoding an N-terminal HA tag in the T7 promoter-driven expression in E. coli. Biotechnol Lett. 2012 Oct;34(10):1841-6.

    [3]. Schembri L, et al. The HA tag is cleaved and loses immunoreactivity during apoptosis. Nat Methods. 2007 Feb;4(2):107-8.

TRAP-6(Synonyms: PAR-1 agonist peptide Thrombin Receptor Activator Peptide 6)

TRAP-6;(Synonyms: PAR-1 agonist peptide; Thrombin Receptor Activator Peptide 6) 纯度: 99.74%

TRAP-6 (PAR-1 agonist peptide),一种多肽片段,是选择性的蛋白酶激活受体 1 (PAR1) 激动剂。TRAP-6 通过凝血酶受体激活人血小板。TRAP-6 对 PAR4 无活性。

TRAP-6amp;;(Synonyms: PAR-1 agonist peptide;  Thrombin Receptor Activator Peptide 6)

TRAP-6 Chemical Structure

CAS No. : 141136-83-6

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ¥1760 In-stock
10 mg ¥1600 In-stock
25 mg ¥3520 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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TRAP-6 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • GPCR/G Protein Compound Library
  • Immunology/Inflammation Compound Library
  • Peptidomimetic Library
  • Targeted Diversity Library
  • Peptide Library

生物活性

TRAP-6 (PAR-1 agonist peptide), a peptide fragment, is a selective protease activating receptor 1 (PAR1) agonist. TRAP-6 activates human platelets via the thrombin receptor. TRAP-6 shows no activity at PAR4[1].

IC50 Target

PAR1[1]

体外研究
(In Vitro)

TRAP-6 (0.01-10 μM) triggers calcium mobilization in Xenopus oocytes heterologously expressing PAR1[1].
TRAP-6 (0.01-10 μM; 30 min) activates human platelets[1].
TRAP-6 (100 μM) does not cause the platelets of rabbits or rats to change shape, aggregate, release granule contents, or form thromboxane[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

TRAP (1 mg/kg; i.v.) produces a biphasic response in blood pressure in inactin-anesthetized rats[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

748.87

Formula

C34H56N10O9

CAS 号

141136-83-6

Sequence

Ser-Phe-Leu-Leu-Arg-Asn

Sequence Shortening

SFLLRN

中文名称

凝血酶受体激活肽6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 100 mg/mL (133.53 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3353 mL 6.6767 mL 13.3535 mL
5 mM 0.2671 mL 1.3353 mL 2.6707 mL
10 mM 0.1335 mL 0.6677 mL 1.3353 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Kahn ML, et, al. Protease-activated receptors 1 and 4 mediate activation of human platelets by thrombin. J Clin Invest. 1999 Mar;103(6):879-87.

    [2]. Kinlough-Rathbone RL, et, al. Rabbit and rat platelets do not respond to thrombin receptor peptides that activate human platelets. Blood. 1993 Jul 1;82(1):103-6.

    [3]. Chintala MS, et, al. Disparate effects of thrombin receptor activating peptide on platelets and peripheral vasculature in rats. Eur J Pharmacol. 1998 May 22;349(2-3):237-43.

OVA Peptide(257-264) TFA

OVA Peptide(257-264) TFA; 纯度: 98.78%

OVA Peptide(257-264) TFA 是 OVA 的 I 类 (Kb)-限制性肽表位,OVA 是由 I 类 MHC 分子 H-2Kb 呈递的来自卵清蛋白的八聚体肽。

OVA Peptide(257-264) TFAamp;;

OVA Peptide(257-264) TFA Chemical Structure

CAS No. : 1262751-08-5

规格 价格 是否有货 数量
1 mg ¥900 In-stock
5 mg ¥3600 In-stock
10 mg ¥5600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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OVA Peptide(257-264) TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

OVA Peptide(257-264) TFA is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide from ovalbumin presented by the class I MHC molecule, H-2Kb.

体外研究
(In Vitro)

TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1077.15

Formula

C47H75F3N10O15

CAS 号

1262751-08-5

Sequence

Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu

Sequence Shortening

SIINFEKL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 20 mg/mL (18.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9284 mL 4.6419 mL 9.2838 mL
5 mM 0.1857 mL 0.9284 mL 1.8568 mL
10 mM 0.0928 mL 0.4642 mL 0.9284 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (1.93 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (1.93 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9.

Cell Assay

TAPl-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide(257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9.

OVA Peptide 323-339(Synonyms: OVA肽)

OVA Peptide 323-339;(Synonyms: OVA肽)

OVA Peptide (323-339) 代表卵清蛋白(OVA)的T和B细胞表位,这对于BALB/c小鼠的瞬时超敏反应的产生和发展是重要的。

OVA Peptide 323-339amp;;(Synonyms: OVA肽)

OVA Peptide 323-339 Chemical Structure

规格 价格 是否有货 数量
1 mg ¥1100 In-stock
5 mg ¥4400 In-stock
10 mg ¥7900 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

OVA Peptide 323-339 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

OVA Peptide (323-339) represents a T and B cell epitope of Ovalbumin (Ova), which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice.

体外研究
(In Vitro)

When pulsed with 0.01 μM of OVA Peptide (323-339), M2-expressing B cells lead to an increase in the number of TH cells mobilizing calcium, compared to M2Y-expressing B cells. To assess if M2-expressing B cells are also able to promote stronger individual responses, we quantified the 405/530 ratio MFI of responding TH cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

OVA Peptide (323-339) represents a T and B cell epitope of OVA, which is important in the generation and development of immediate hypersensitivity responses in BALB/c mice. Daily aerosolization of OVA Peptide (323-339) for 20 minutes over a period of 10 days has been as effective in the stimulation of a serum anti-OVA IgE antibody response as sensitization to native OVA by the same route. After sensitization to native OVA, the majority of the IgE anti-OVA response is directed against OVA Peptide (323-339). Evaluated by hematoxylin/eosin and major basic protein immunohistochemical stainings, OVA and OVA Peptide (323-339) induce similar lung inflammation. Interestingly, significant serum total IgE and OVA-specific IgE are observed in OVA mice when compared to saline control. OVA Peptide (323-339) mice show higher serum OVA-specific IgE, OVA Peptide (323-339)-specific IgE, IL-4 and lower IFN-γ similar to OVA mice. The proliferative response to OVA is found in cultured splenocytes of both OVA and OVA Peptide (323-339) mice, while the similar proliferative response to OVA Peptide (323-339) is only observed in the splenocytes of OVA Peptide (323-339)-sensitized and challenged mice. Although OVA Peptide (323-339) induces a Th2-like response in the mouse model as does OVA, OVA Peptide (323-339) has clearly limited immunogenic potency to activate OVA-sensitized and challenged mice splenocytes, unlike OVA[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1773.91

Formula

C74H120N26O25

Sequence

Ile-Ser-Gln-Ala-Val-His-Ala-Ala-His-Ala-Glu-Ile-Asn-Glu-Ala-Gly-Arg

Sequence Shortening

ISQAVHAAHAEINEAGR

中文名称

OVA肽

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : ≥ 50 mg/mL (28.19 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5637 mL 2.8186 mL 5.6373 mL
5 mM 0.1127 mL 0.5637 mL 1.1275 mL
10 mM 0.0564 mL 0.2819 mL 0.5637 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Fontinha D, et al. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS One. 2015 Nov 6;10(11):e0142540.

    [2]. Sun LZ, et al. Comparison between ovalbumin and ovalbumin peptide 323-339 responses in allergic mice: humoral and cellular aspects. Scand J Immunol. 2010 May;71(5):329-35.

Cell Assay
[1]

A20 B cell lines are loaded, or not, with different concentrations of OVA Peptide (323-339) overnight. Cells are then conjugated with purified mouse CD4+ T cells and incubated for 20 h. After the 20 h incubation period, supernatant is recovered and stored at -20°C. IFN-γ production is quantified in the supernatants by sandwich ELISA. 96-well plates are analyzed. T cells cultured for two to four days in the presence of 3 μg/mL of anti-CD3 antibody are used as positive control of T cell activation. B cells cultured for 48 h in the presence of 2.5 μg/mL of anti-CD40 antibody and 5 μg/mL of the F(ab’)2/F(ab) portion of an anti-mouse IgG antibody are used as positive control for B cell activation[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice[2]
Fifty-one 8-week-old female BALB/c mice are randomly divided into three groups: OVA, OVA 323-339 and saline. They are intraperitoneally injected with 25 μg OVA or OVA Peptide (323-339) absorbed on 300 μg Alum or saline on days 0, 7, 14. On days 21-23, all groups are challenged intranasally with 20 μL of 1% OVA, 1% OVA Peptide (323-339) and saline, respectively. On days 0, 7, 14, mice are intraperitoneally injected with 25 μg OVA or OVA Peptide (323-339) absorbed on 300 μg Alum, or saline; on days 21-23, all groups are challenged intranasally with either 20 μL of 1% OVA, 1% OVA Peptide (323-339) or saline. On day 28, after killing, splenocytes are isolated and cultured under the stimulus of each allergen or medium.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Fontinha D, et al. Murid Gammaherpesvirus Latency-Associated Protein M2 Promotes the Formation of Conjugates between Transformed B Lymphoma Cells and T Helper Cells. PLoS One. 2015 Nov 6;10(11):e0142540.

    [2]. Sun LZ, et al. Comparison between ovalbumin and ovalbumin peptide 323-339 responses in allergic mice: humoral and cellular aspects. Scand J Immunol. 2010 May;71(5):329-35.

iRGD peptide(Synonyms: c(CRGDKGPDC))

iRGD peptide;(Synonyms: c(CRGDKGPDC)) 纯度: 99.03%

iRGD peptide 是一种由 9 个氨基酸组成的环肽,先与 av integrins 结合,随后酶解产生 CRGDK/R 与 神经纤毛蛋白-1 (neuropilin-1) 相互作用,从而促进药物的组织渗透,具有靶向肿瘤、肿瘤渗透的作用。

iRGD peptideamp;;(Synonyms: c(CRGDKGPDC))

iRGD peptide Chemical Structure

CAS No. : 1392278-76-0

规格 价格 是否有货 数量
1 mg ¥900 In-stock
5 mg ¥3600 In-stock
10 mg ¥6200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

iRGD peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library
  • Macrocyclic Compound Library
  • Peptide Library

生物活性

iRGD peptide is a 9-amino acid cyclic peptide, triggers tissue penetration of drugs by first binding to av integrins, then proteolytically cleaved in the tumor to produce CRGDK/R to interact with neuropilin-1, and has tumor-targeting and tumor-penetrating properties.

IC50 Target

Integrin[1]

体外研究
(In Vitro)

iRGD peptide-mediated tumor penetration occurs in three steps: binding to αv-integrins on tumor vasculature or tumor cells, exposure by proteolysis of a C-terminal motif that binds to neuropilin-1 (NRP-1) and cell internalization. iRGD peptide inserted in the ICOVIR15K fiber C terminus enhances binding and internalization only in MCF7 cells, which express NRP-1 and integrins. iRGD insertion does not impair virus infection and replication[1]. iRGD peptide alone has no obvious effect on gastric cancer cells, and when combined with 5-FU, iRGD peptide (0.3 μmol/mL) enhances the chemotherapy efficacy of 5-FU on gastric cancer cells through NRP1[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

iRGD inserted in the oncolytic adenovirus ICOVIR15K (ICOVIR15K-iRGD) enhances early adenovirus dissemination through the tumor mass and elevates the antitumor effect in mice[1]. iRGD (4 mmol/kg, i.v.) in combination with 5-FU significantly suppresses the tumor growth in nude mice bearing human gastric cancer cells[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

948.04

Formula

C35H57N13O14S2

CAS 号

1392278-76-0

Sequence

Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys (Disulfide bridge:Cys1-Cys9)

Sequence Shortening

CRGDKGPDC (Disulfide bridge:Cys1-Cys9)

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : ≥ 50 mg/mL (52.74 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0548 mL 5.2740 mL 10.5481 mL
5 mM 0.2110 mL 1.0548 mL 2.1096 mL
10 mM 0.1055 mL 0.5274 mL 1.0548 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Puig-Saus C, et al. iRGD tumor-penetrating peptide-modified oncolytic adenovirus shows enhanced tumor transduction, intratumoral dissemination and antitumor efficacy. Gene Ther. 2014 Aug;21(8):767-74.

    [2]. Zhang L, et al. Combination of NRP1-mediated iRGD with 5-fluorouracil suppresses proliferation, migration and invasion of gastric cancer cells. Biomed Pharmacother. 2017 Sep;93:1136-1143.

Animal Administration
[2]

Mice[2]
12 male BALB/c nude mice (4-week-old) are assigned to 4 groups with 3 mice in each group. Among them,two groups are subcutaneously injected into the flanks by 3 × 106 HCG27 cells, the other two groups are conducted by NCI-N87 cells. Experimental groups are intravenously injected by 5-FU (25 mg/kg) mixed with iRGD peptide (4 mmol/kg) at every three days for 4 weeks while control groups are treated by 5-FU (25 mg/kg) mixed with PBS. And tumor volume is computed every 1 week with a digital vernier caliper using the following formula: tumor volume = (length × width2)/2[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Puig-Saus C, et al. iRGD tumor-penetrating peptide-modified oncolytic adenovirus shows enhanced tumor transduction, intratumoral dissemination and antitumor efficacy. Gene Ther. 2014 Aug;21(8):767-74.

    [2]. Zhang L, et al. Combination of NRP1-mediated iRGD with 5-fluorouracil suppresses proliferation, migration and invasion of gastric cancer cells. Biomed Pharmacother. 2017 Sep;93:1136-1143.

AMARA peptide TFA

AMARA peptide TFA; 纯度: 98.38%

AMARA peptide (TFA) 是盐诱导激酶 (SIK) 和腺苷酸活化蛋白激酶 (AMPK) 的底物。

AMARA peptide TFAamp;;

AMARA peptide TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥800 In-stock
5 mg ¥1800 In-stock
10 mg ¥2800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

AMARA peptide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

AMARA peptide (TFA) is a substrate for salt-inducible kinase (SIK) and adenosine monophosphate activated protein kinase (AMPK).

分子量

1640.84

Formula

C64H116F3N27O18S

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 50 mg/mL (30.47 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6094 mL 3.0472 mL 6.0944 mL
5 mM 0.1219 mL 0.6094 mL 1.2189 mL
10 mM 0.0609 mL 0.3047 mL 0.6094 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Shan T, et al. Lkb1 controls brown adipose tissue growth and thermogenesis by regulating the intracellular localization of CRTC3. Nat Commun. 2016 Jul 27;7:12205.

Peptide YY (PYY) (3-36), porcine TFA

Peptide YY (PYY) (3-36), porcine TFA; 纯度: 99.21%

Peptide YY (PYY) (3-36), porcine TFA 是一种肠胃激素肽,作为 Y2 受体的激动剂,能够降低食欲。

Peptide YY (PYY) (3-36), porcine TFAamp;;

Peptide YY (PYY) (3-36), porcine TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥1100 In-stock
5 mg ¥3300 In-stock
10 mg ¥5300 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Peptide YY (PYY) (3-36), porcine TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Peptide YY (PYY) (3-36), porcine TFA is a gut hormone peptide that acts as a Y2 receptor agonist to reduce appetite[1].

分子量

4094.44

Formula

C176H272N52O54.C2HF3O2

Sequence

Ala-Lys-Pro-Glu-Ala-Pro-Gly-Glu-Asp-Ala-Ser-Pro-Glu-Glu-Leu-Ser-Arg-Tyr-Tyr-Ala-Ser-Leu-Arg-His-Tyr-Leu-Asn-Leu-Val-Thr-Arg-Gln-Arg-Tyr

Sequence Shortening

AKPEAPGEDASPEELSRYYASLRHYLNLVTRQRY-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 100 mg/mL (24.42 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2442 mL 1.2212 mL 2.4423 mL
5 mM 0.0488 mL 0.2442 mL 0.4885 mL
10 mM 0.0244 mL 0.1221 mL 0.2442 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Addison ML, et al. A role for metalloendopeptidases in the breakdown of the gut hormone, PYY 3-36. Endocrinology. 2011 Dec;152(12):4630-40.

Fibronectin Adhesion-promoting Peptide(Synonyms: Heparin Binding Peptide)

Fibronectin Adhesion-promoting Peptide;(Synonyms: Heparin Binding Peptide) 纯度: 99.13%

Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide) 是一种肝素结合氨基酸序列,这段序列存在于纤连蛋白 C-端的肝素结合域中。Fibronectin Adhesion-promoting Peptide 促进间充质干细胞 (MSC) 球状体组装成更大的聚集体。Fibronectin Adhesion-promoting Peptide 通过与细胞的肝素结合区反应直接促进内皮细胞的粘附、扩散和迁移。

Fibronectin Adhesion-promoting Peptideamp;;(Synonyms: Heparin Binding Peptide)

Fibronectin Adhesion-promoting Peptide Chemical Structure

CAS No. : 125720-21-0

规格 价格 是否有货 数量
5 mg ¥2400 In-stock
10 mg ¥3800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Fibronectin Adhesion-promoting Peptide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Macrocyclic Compound Library
  • Peptide Library

生物活性

Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide) is one of the heparin-binding amino acid sequences found in the carboxy-terminal heparin-binding domain of fibronectin. It promotes assembly of mesenchymal stem cell (MSC) spheroids into larger aggregates. Fibronectin Adhesion-promoting Peptide directly promotes the adhesion, spreading, and migration of endothelial cells by reacting with heparin binding domains of cells[1][2].

体外研究
(In Vitro)

GRGDS and Fibronectin Adhesion-promoting Peptide (Heparin Binding Peptide: WQPPRARI) micropatterns increase the endothelialisation of prosthetic materials in vitro. Fibronectin Adhesion-promoting Peptide also enhances haptotactic cell migration. The anti-apoptotic effects of fibronectin require the presence of the WQPPRARI sequence, which may be due to synergistic survival signals mediated by this sequence and by the RGD motif[1].
RDGS and Fibronectin Adhesion-promoting Peptide promote cell adhesion through different activation pathways that induce different forms of adhesion which can be highlighted through the analysis of focal and fibrillar contacts[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1023.19

Formula

C47H74N16O10

CAS 号

125720-21-0

Sequence

Trp-Gln-Pro-Pro-Arg-Ala-Arg-Ile

Sequence Shortening

WQPPRARI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 2 mg/mL (1.95 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9773 mL 4.8867 mL 9.7734 mL
5 mM
10 mM

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Hoesli CA, et al. A fluorophore-tagged RGD peptide to control endothelial cell adhesion to micropatterned surfaces. Biomaterials. 2014 Jan;35(3):879-90.

    [2]. Chollet C, et al. Impact of peptide micropatterning on endothelial cell actin remodeling for cell alignment under shear stress. Macromol Biosci. 2012 Dec;12(12):1648-59.

    [3]. Lei J, Murphy WL, et al. Combination of Heparin Binding Peptide and Heparin Cell Surface Coatings for Mesenchymal Stem Cell Spheroid Assembly. Bioconjug Chem. 2018 Apr 18;29(4):878-884.

RAGE antagonist peptide TFA

RAGE antagonist peptide TFA; 纯度: 99.04%

RAGE antagonist peptide TFA 是一种晚期糖基化终产物 (RAGE) 拮抗剂。RAGE antagonist peptide TFA 可阻断 RAGE 和几个重要配体 HMGB-1,S100P,S100A4 的结合。RAGE antagonist peptide TFA 具有抗肿瘤和抗炎活性。

RAGE antagonist peptide TFAamp;;

RAGE antagonist peptide TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥890 In-stock
5 mg ¥2700 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

RAGE antagonist peptide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Neuronal Signaling Compound Library
  • Anti-Cancer Compound Library
  • Anti-Alzheimer’s Disease Compound Library
  • Neurodegenerative Disease-related Compound Library
  • Peptide Library

生物活性

RAGE antagonist peptide TFA is an advanced glycation end products (RAGE) antagonist. RAGE antagonist peptide TFA prevents RAGE from binding with several of its most important ligands, including HMGB-1, S100P, and S100A4. RAGE antagonist peptide TFA possesses anti-tumor and anti-inflammatory activities[1][2].

体外研究
(In Vitro)

RAGE antagonist peptide TFA (RAP) reduces the ability of the ligands to stimulate RAGE activation of NFκB in cancer cells in vitro[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

RAGE antagonist peptide TFA (RAP, 100 µg) inhibits RAGE-mediated Basal NFκB Activity in PDAC cells in vivo[1].
RAGE antagonist peptide TFA (RAP) reduces the growth and metastasis of pancreatic tumors and also inhibited glioma tumor growth[1].
In mice bearing asthma, RAGE antagonist peptide TFA (RAP; 4 mg/kg; i.p.) blunts airway reactivity, airway inflammation and goblet cell metaplasia, and decreases release of Th2 cytokines. RAGE antagonist peptide TFA also reduces total, cytoplasmic and nuclear levels of β-catenin, enhances β-catenin phosphorylation at Ser33/37/Thr41, which triggers ubiquitination, down-regulated expression of β-catenin targeted genes, and tends to keep β-catenin at the cytomembrane, shifting β-catenin from a signalling active pattern to an adhesive function[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Cancer cells expressing the NFκB-luc reporter implanted into immune-deficient mice[1].
Dosage: 100 µg
Administration: Intratumoral delivery (or intraperitoneally).
Result: Systemic administration caused a substantial reduction (p<0.05) in the NFκB signal 5 h after injection.

分子量

1386.58

Formula

C59H102F3N13O19S

Sequence Shortening

Ac-ELKVLMEKEL-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 25 mg/mL (18.03 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7212 mL 3.6060 mL 7.2120 mL
5 mM 0.1442 mL 0.7212 mL 1.4424 mL
10 mM 0.0721 mL 0.3606 mL 0.7212 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Thiruvengadam Arumugam, et al. S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis. Clin Cancer Res. 2012 Aug 15;18(16):4356-64.

    [2]. Lihong Yao, et al. The receptor for advanced glycation end products is required for β-catenin stabilization in a chemical-induced asthma model. Br J Pharmacol. 2016 Sep;173(17):2600-13.