G12(Synonyms: Ras 5-17)

G12;(Synonyms: Ras 5-17)

G12 (Ras 5-17) 是由氨基酸 5-17 (KLVVVGAGGVGKS) 组成的野生型 Ras 肽。G12 可用作突变 Ras 肽研究的对照 (例如 V12)。

G12amp;;(Synonyms: Ras 5-17)

G12 Chemical Structure

CAS No. : 162550-82-5

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生物活性

G12 (Ras 5-17) is a wild-type Ras peptide consisted of amino acids 5-17 (KLVVVGAGGVGKS). G12 can be used as a control of mutant Ras peptides studies (such V12)[1].

分子量

1170.40

Formula

C52H95N15O15

CAS 号

162550-82-5

Sequence Shortening

KLVVVGAGGVGKS

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Abrams SI, et al. Peptide-specific activation of cytolytic CD4+ T lymphocytes against tumor cells bearing mutated epitopes of K-ras p21 [published correction appears in Eur J Immunol 1995 Dec;25(12):3525]. Eur J Immunol. 1995;25(9):2588-2597.

S12 TFA

S12 TFA;

G12 (Ras 5-17) 是由氨基酸 5-17 (KLVVVGAGGVGKS) 组成的野生型 Ras 肽。G12 可用作突变 Ras 肽研究的对照 (例如 V12)。

S12 TFAamp;;

S12 TFA Chemical Structure

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生物活性

G12 (Ras 5-17) is a wild-type Ras peptide consisted of amino acids 5-17 (KLVVVGAGGVGKS). G12 can be used as a control of mutant Ras peptides studies (such V12)[1].

分子量

1314.45

Formula

C55H98F3N15O18

Sequence Shortening

KLVVVGASGVGKS

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Luo Y, et al. Mutated ras p21 as a target for cancer therapy in mouse transitional cell carcinoma. J Urol. 1999;162(4):1519-1526.

G280-9

G280-9;

G280-9 是含有 9 个氨基酸的天然表位肽。G280-9 是在黑素瘤上表达的相关靶蛋白。

G280-9amp;;

G280-9 Chemical Structure

CAS No. : 156761-76-1

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生物活性

G280-9 is a 9 amino acid native epitope peptide. G280-9 is a relevant target expressed on melanoma.

分子量

946.05

Formula

C44H67N9O14

CAS 号

156761-76-1

Sequence

Tyr-Leu-Glu-Pro-Gly-Pro-Val-Thr-Ala

Sequence Shortening

YLEPGPVTA

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. Pass HA, et al. Immunization of patients with melanoma peptide vaccines: immunologic assessment using the ELISPOT assay. Cancer J Sci Am. 1998 Sep-Oct;4(5):316-23.

OVA G4 peptide

OVA G4 peptide;

OVA G4 peptide 是激动剂卵白蛋白肽 SIINFEKL (OVA) (257-264) 的变体。SIINFEKL 通常用于刺激卵白蛋白特异性T细胞,并测试新的疫苗助剂是否能形成稳定的水凝胶。

OVA G4 peptideamp;;

OVA G4 peptide Chemical Structure

CAS No. : 148274-82-2

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OVA G4 peptide 的其他形式现货产品:

OVA G4 peptide TFA

生物活性

OVA G4 peptide is a variant of the agonist ovalbumin (OVA) peptide SIINFEKL (257-264). SIINFEKL is routinely used to stimulate ovalbumin-specific T cells and to test new vaccine adjuvants can form a stable hydrogel[1][2].

分子量

906.08

Formula

C43H71N9O12

CAS 号

148274-82-2

Sequence

Ser-Ile-Ile-Gly-Phe-Glu-Lys-Leu

Sequence Shortening

SIIGFEKL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. François P, et al. Phenotypic model for early T-cell activation displaying sensitivity, specificity, and antagonism. Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):E888-97.

    [2]. Kamalov M,et al. Ovalbumin Epitope SIINFEKL Self-Assembles into a Supramolecular Hydrogel. Sci Rep. 2019 Feb 25;9(1):2696.

Apigenin-7-O-(2G-rhamnosyl)gentiobioside

天然产物 黄酮类 Flavonoids

Apigenin-7-O-(2G-rhamnosyl)gentiobioside 

Apigenin-7-O-(2G-rhamnosyl)gentiobioside 是从Lonicera gracilipes var. glandulosa 中提取的到的一种黄酮类苷化合物。

Apigenin-7-O-(2G-rhamnosyl)gentiobioside

Apigenin-7-O-(2G-rhamnosyl)gentiobioside Chemical Structure

CAS No. : 174284-20-9

规格 价格 是否有货 数量
5 mg ¥3360 In-stock
10 mg ¥5710 In-stock
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100 mg   询价  

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Apigenin-7-O-(2G-rhamnosyl)gentiobioside 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Glycoside Compound Library
  • Phenols Library
  • Flavonoids Library

生物活性

Apigenin-7-O-(2G-rhamnosyl)gentiobioside is a flavone glycosides from Lonicera gracilipes var. glandulosa[1].

分子量

740.66

Formula

C33H40O19

CAS 号

174284-20-9

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

参考文献
  • [1]. Masao Kikuchi, et al. Flavone Glycosides from Lonicera gracilipes var. glandulosa. J Nat Prod 1996, 59, 314-315.

Rhodamine 6G(Synonyms: 罗丹明6G Basic Red 1)

Rhodamine 6G;(Synonyms: 罗丹明6G; Basic Red 1) 纯度: ge;98.0%

Rhodamine 6G是罗丹明类似物,可用于Pgp外排测定试验中。它可用于MRP1-介导外排的动力学试验中,以及作为激光染料和具有线粒体探针的潜力。

Rhodamine 6Gamp;;(Synonyms: 罗丹明6G; Basic Red 1)

Rhodamine 6G Chemical Structure

CAS No. : 989-38-8

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥550 In-stock
250 mg ¥500 In-stock
1 g ¥800 In-stock
5 g ¥1200 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

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Rhodamine 6G 相关产品

bull;相关化合物库:

  • FDA Approved amp; Pharmacopeial Drug Library
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus
  • Anti-Cancer Compound Library
  • Clinical Compound Library

生物活性

Rhodamine 6G is a rhodamine analog useful in Pgp efflux assays. It can be used in characterizing the kinetics of MRP1- mediated efflux, and as a laser dye and potential mitochondrial probe.

体外研究
(In Vitro)

Rhodamine 6G, also known as Rhodamine 590, is widely used as a lasing medium and as a fluorescence tracer. For use in dye lasers, it is dissolved in methanol, ethanol and a variety of other organic solvents. In environmental flow studies, the tracer medium is typically water. In ethanol, the absorption of rhodamine 6G ranges between 440 nm and 570 nm, with the peak at 530 nm. It is thus ideally suited for pumping by frequency doubled Nd:YAG lasers at 532 nm, copper vapor lasers at 511 nm, and argon-ion lasers at 514 nm. The resulting emission spectrum varies from about 510 nm to around 710 nm with the peak at 550 nm depending on the solvent and the dye concentration. However, the laser emission range is much narrower, from around 560 nm to 610 nm with the peak wavelength around 575 nm. Energy conversion efficiencies greater than 50% are achievable. In order to select the optimal solvent and dye concentration, good knowledge of their effects is a pre-requisite. Rhodamine 6G in DMSO shows a distinct behavior exhibiting only 41% of the fluorescence intensity of the methanol case with an 11 nm red-shifted wavelength. Relatively small changes of the fluorescence spectrum are observed for the different solvents; the highest fluorescence intensity is observed for methanol and lowest for DMSO. The shortest peak wavelength is found in methanol (568 nm) and the longest in DMSO (579 nm). Changing the dye concentration provides tunability between 550 nm in the dilute case and 620 nm at high concentration, at which point the fluorescence spectrum indicates the formation of rhodamine 6G aggregates[1]. Rhodamine 6G is a fluorescent dye capable of penetrating a living cell. Upon entering the cell, Rhodamine 6G binds to the inner membranes of mitochondria. Based on these observations, it has been proposed that Rhodamine dyes may be used for producing fluorescent images of mitochondria with low background noise and high resolution. Extremely low concentrations of Rhodamine 6G appear to selectively destroy malignant cells in culture, sparing the normal cell populations[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Melanoma-transplanted mice receiving Rhodamine 6G demonstrate prolonged survival, improved clinical parameters, inhibited tumor growth and metastases count, compared to their untreated counterparts. Twice-a-week 10-6M Rhodamine 6G regimen yield the most prominent results[2]. The Rhodamine-6G enters the circulatory system and labels leukocytes. It is possible to monitor changes in the interactions between leukocytes and the endothelium by determining the numbers of rolling and adhering leukocytes as well as the total flux of these cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

479.01

Formula

C28H31ClN2O3

CAS 号

989-38-8

中文名称

罗丹明6G

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (52.19 mM; Need ultrasonic)

H2O : 10 mg/mL (20.88 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0876 mL 10.4382 mL 20.8764 mL
5 mM 0.4175 mL 2.0876 mL 4.1753 mL
10 mM 0.2088 mL 1.0438 mL 2.0876 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.5 mg/mL (5.22 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (5.22 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: 2.5 mg/mL (5.22 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (5.22 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Zehentbauer FM, et al. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects. Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:147-51.

    [2]. Kutushov M, et al. Low concentrations of Rhodamine 6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73.

    [3]. Jain RK, et al. Measuring leukocyte-endothelial interactions in mice. Cold Spring Harb Protoc. 2013 Jun 1;2013(6):561-3.

Cell Assay
[2]

Malignant cells and normal control cultures are seeded in equal (protein adjusted) cell amounts into 6-well tissue culture plates. The cells are pulsed with 25µCi/mL of 3H-Thymidine and immediately treated with Rhodamine 6G at the fixed concentration of 1 μM for 24h, 48h, 72h or 5 days (120h). Following 24h, 48h, 72h or 5 days, the excessive radioactive material is ished out with PBS. The cell samples are transferred into polystyrene vials containing 4 ml scintillation liquid, and their radioactivity counted in a β-counter. Total cell protein is assessed by Bradford’s assay[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[2]

Mice: C57Bl mice are implanted with B16-F10 melanoma and treated with Rhodamine 6G (1, 0.1, 0.01 μM) at different dosage/time regimens. Viability and proliferation of cultured tumor cells are analyzed[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Zehentbauer FM, et al. Fluorescence spectroscopy of Rhodamine 6G: concentration and solvent effects. Spectrochim Acta A Mol Biomol Spectrosc. 2014;121:147-51.

    [2]. Kutushov M, et al. Low concentrations of Rhodamine 6G selectively destroy tumor cells and improve survival of melanoma transplanted mice. Neoplasma. 2013;60(3):262-73.

    [3]. Jain RK, et al. Measuring leukocyte-endothelial interactions in mice. Cold Spring Harb Protoc. 2013 Jun 1;2013(6):561-3.

荧光染料Cibacron Blue 3G-A

荧光染料Dye Reagents Cibacron Blue 3G-A;

Cibacron Blue 3G-A 是一种蒽醌染料,可以抑制 R46 β-lactamaseKi 值为 1.2 uM。

Cibacron Blue 3G-A

Cibacron Blue 3G-A Chemical Structure

CAS No. : 84166-13-2

规格 价格 是否有货
10 mg ¥500 询问价格 货期
25 mg ¥700 询问价格 货期
50 mg ¥900 询问价格 货期
100 mg ¥1500 询问价格 货期

* Please select Quantity before adding items.

生物活性

Cibacron Blue 3G-A is an anthraquinone dye, inhibits the R46 β-lactamase with a Ki value of 1.2 uM[1].

IC50 Target

IC50: anthraquinone dye[1]

体外研究
(In Vitro)

Cibacron Blue 3G-A is a structural analogy between the dye and NADH, it interacts with (di)nucleotide-dependent enzymes and can be a standard tool for studying their active sites[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

774.16

Formula

C29H20ClN7O11S3

CAS 号

84166-13-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Monaghan C, et al. The interaction of anthraquinone dyes with the plasmid-mediated OXA-2 beta-lactamase.Biochem J. 1982 Aug 1;205(2):413-7.

    [2]. Monaghan C, et al. The interaction of anthraquinone dyes with the plasmid-mediated OXA-2 beta-lactamase.Biochem J. 1982 Aug 1;205(2):413-7.

Platycoside G1(Synonyms: Deapi-platycoside E)

天然产物 糖类和糖苷 Saccharides and Glycosides

Platycoside G1 (Synonyms: Deapi-platycoside E) 纯度: ≥98.0%

Platycoside G1,是桔梗中发现的一种天然产物,是一种三萜皂苷。Platycoside G1 具有较强的抗氧化活性。

Platycoside G1(Synonyms: Deapi-platycoside E)

Platycoside G1 Chemical Structure

CAS No. : 849758-42-5

规格 价格 是否有货 数量
1 mg ¥3430 In-stock
5 mg ¥10290 In-stock
10 mg   询价  
50 mg   询价  

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Platycoside G1 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus

生物活性

Platycoside G1, a natural product found in Platycodon grandiflorum, is a triterpenoid saponin. Platycoside G1 has potent antioxidant activities[1].

分子量

1417.49

Formula

C64H104O34

CAS 号

849758-42-5

中文名称

桔梗皂苷G1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

参考文献
  • [1]. Ryu CS, et al. Evaluation of the total oxidant scavenging capacity of saponins isolated from Platycodon grandiflorum. Food Chem. 2012 May 1;132(1):333-7.

G3-C12 TFA

G3-C12 TFA; 纯度: 99.45%

G3-C12 (TFA) 是 galectin-3 结合肽,Kd 值为 88 nM,具有抗肿瘤活性。

G3-C12 TFAamp;;

G3-C12 TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥900 In-stock
5 mg ¥2200 In-stock
10 mg ¥3300 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

G3-C12 TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

G3-C12 (TFA) is a galectin-3 binding peptide, with Kd of 88 nM, and shows anticancer activity.

IC50 Target

Kd: 88 nM (Galectin-3)[1].

体外研究
(In Vitro)

G3-C12 (TFA) is a galectin-3 binding peptide, with high affinity (Kd) of 88 nM, but shows no affinity for other galectin family members or to other lectins. G3-C12 (TFA) bearing opolymers N-(2-hydroxypropyl)methacrylamide (HPMA) potently and selectively targets colorectal cancer (CRC) tumour cells over-expressing galectin-3 and displays superior targetability to galectin-3 compared to the galactose bearing copolymer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1873.00

Formula

C74H115N23O23S2.C2HF3O2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : ≥ 50 mg/mL (26.70 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5339 mL 2.6695 mL 5.3390 mL
5 mM 0.1068 mL 0.5339 mL 1.0678 mL
10 mM 0.0534 mL 0.2670 mL 0.5339 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Kopansky E, et al. Peptide-directed HPMA copolymer-doxorubicin conjugates as targeted therapeutics for colorectal cancer. J Drug Target. 2011 Dec;19(10):933-43.

Agilent-Technologies氘灯G1314-60100 G1314-60100


Agilent-Technologies氘灯G1314-60100

Agilent-Technologies氘灯G1314-60100 Agilent安捷伦G1314-60100替代1100/1120/1200/1220/1260VWD氘灯 Agilent-Technologies氘灯G1314-60100

Agilent-Technologies氘灯G1314-60100

安捷伦检测器灯:

• 按严格的性能指标和质量标准制造

• 采用增加光强度并降低噪声的设计

• 经过严格测试,灯与灯之间的变化率低

• 稳定、耐用、寿命长,降低用户的成本

 

Agilent安捷伦氘灯匹配表

检测器

部件号

原装灯的部件号

二级管振列检测器(DAD

G1315A/B

2140-0813

G1315C/D

2140-0820

G4212A/B

5190-0917

多波长检测器(MWD

G1365A/B

2140-0813/2140-0590

G1365C/D

2140-0820

可变波长检测器(VWD

G1314A/B/C

G1314-60100

G1314D/E/F

G1314-60101

UV VIS

8453A

2140-0605

钨灯

G1315A/B/C/D

G1365A/B/C/D

G1103-60001