生化分析试剂 Biochemical Assay Reagents Brilliant blue G-250;
Brilliant Blue G-250 是一种常用于 SDS-PAGE 分离蛋白质可视化的染料，染色过程简单，定量高。在 Bradford 蛋白质分析中，由于 Brilliant Blue G-250 与蛋白质结合，蛋白质浓度由 595 nm 处的吸光度确定。Brilliant Blue G-250 是一种安全、高选择性的 P2×7R 拮抗剂，有望使 NLRP3炎症体失活。
Brilliant blue G-250 Chemical Structure
CAS No. : 6104-58-1
规格
是否有货
100 mg
;
询价
;
250 mg
;
询价
;
500 mg
;
询价
;
* Please select Quantity before adding items.
生物活性
Brilliant Blue G-250 is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. In the Bradford protein assay, protein concentrations are determined by the absorbance at 595 nm due to the binding of Brilliant Blue G-250 to proteins. Brilliant Blue G-250 is a safe highly selective P2×7R antagonist with promising consequent inactivation of NLRP3 inflammasome[1][2][3].
分子量
854.02
Formula
C47H48N3NaO7S2
CAS 号
6104-58-1
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Dyballa N, Metzger S. Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels. J Vis Exp. 2009;(30):1431. Published 2009 Aug 3.
[2]. Han XX, et al. Highly sensitive protein concentration assay over a wide range via surface-enhanced Raman scattering of Coomassie brilliant blue. Anal Chem. 2010;82(11):4325-4328.
[3]. Zohny MH, et al. Coomassie brilliant blue G-250 dye attenuates bleomycin-induced lung fibrosis by regulating the NF-κB and NLRP3 crosstalk: A novel approach for filling an unmet medical need [published online ahead of print, 2022 Feb 21]. Biomed Pharmacother. 2022;148:112723.
Direct Blue 1;(Synonyms: Chicago Sky Blue 6B) 纯度: ge;95.0%
Direct Blue 1 (Chicago Sky Blue 6B) 是免疫荧光组织化学中背景自体荧光的一种复染料。Direct Blue 1 在结构上与谷氨酸盐有关，是一种有效且具有竞争性的 VGLUT 抑制剂，不影响质膜转运体。Direct Blue 1 是第一个能够抑制 Aβ 结合的小分子 PrP 配体。
Direct Blue 1 Chemical Structure
CAS No. : 2610-05-1
规格
价格
是否有货
数量
10;mM;*;1 mL in DMSO
￥550
In-stock
500 mg
￥500
In-stock
1 g
;
询价
;
5 g
;
询价
;
* Please select Quantity before adding items.
Direct Blue 1 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
Direct Blue 1 (Chicago Sky Blue 6B) is a counterstain for background autofluorescence in immunofluorescence histochemistry. Direct Blue 1, structurally related to glutamate, is a potent and competitive VGLUT inhibitor without affecting plasma membrane transporters. Direct Blue 1 is the first small molecule PrP ligand capable of inhibiting Aβ binding[1].
分子量
992.80
Formula
C34H24N6Na4O16S4
CAS 号
2610-05-1
运输条件
Room temperature in continental US; may vary elsewhere.
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Lodha N, Poojary SA. A Novel Contrast Stain for the Rapid Diagnosis of Pityriasis Versicolor: A Comparison of Chicago Sky Blue 6B Stain, Potassium Hydroxide Mount and Culture. Indian J Dermatol. 2015;60(4):340-344.
[2]. Yu G, et al. The antinociceptive effects of intracerebroventricular administration of Chicago sky blue 6B, a vesicular glutamate transporter inhibitor. Behav Pharmacol. 2013;24(8):653-658.
[3]. Risse E, et al. Identification of a Compound That Disrupts Binding of Amyloid-β to the Prion Protein Using a Novel Fluorescence-based Assay. J Biol Chem. 2015;290(27):17020-17028.
TMB (BM blue) is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).
分子量
240.34
Formula
C16H20N2
CAS 号
54827-17-7
运输条件
Room temperature in continental US; may vary elsewhere.
TMB monosulfate;(Synonyms: BM blue monosulfate; Sure Blue TMB monosulfate) 纯度: 99.88%
TMB monosulfate是免疫组化和ELISA的显色底物。
TMB monosulfate Chemical Structure
CAS No. : 54827-18-8
规格
价格
是否有货
数量
Free Sample (0.1-0.5 mg)
;
Apply now
;
10;mM;*;1 mL in DMSO
￥550
In-stock
100 mg
￥500
In-stock
200 mg
;
询价
;
500 mg
;
询价
;
* Please select Quantity before adding items.
TMB monosulfate 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
TMB monosulfate is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualizing reagent used in enzyme-linked immunosorbent assays (ELISA).
分子量
338.42
Formula
C16H22N2O4S
CAS 号
54827-18-8
运输条件
Room temperature in continental US; may vary elsewhere.
Leucomethylene blue mesylate;(Synonyms: TRx0237 mesylate; Methylene blue leuco base mesylate) 纯度: 98.75%
Leucomethylene blue (TRx0237) mesylate 是具有口服活性的二代 tau 蛋白聚集的抑制剂 (Ki 值为0.12 μM)，可用于阿尔兹海默症的研究。Leucomethylene blue mesylate 是 Methylene blue (亚甲基蓝)的一种还原形式, 亚甲基蓝是一种化学指示剂和生物染色剂。
Leucomethylene blue (TRx0237) mesylate, an orally active second-generation tau protein aggregation inhibitor (Ki of 0.12 μM), could be used for the study of Alzheimer’s Disease. Leucomethylene blue mesylate is a common reduced form of Methylene Blue, Methylene Blue is a member of the thiazine class of dyes[1][2][3].
IC50 Target
Ki: 0.12 μM (tau)[3].
体外研究 (In Vitro)
Leucomethylene blue (100 nM, 48 h) mesylate not only decreases the tau and p-tau expression levels, but also reversed the promoting effects of Aβ25-35 on orexin A and adenosine A1R expression levels[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis[2]
Cell Line:
Human SH-SY5Y cell line.
Concentration:
100 nM.
Incubation Time:
48 h.
Result:
Aβ25-35 and TRx 0237 co-treatment significantly reversed the promoting effects of Aβ25-35 on tau, p-tau, orexin A and adenosine A1R expression.
分子量
477.62
Formula
C18H27N3O6S3
CAS 号
1236208-20-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
4deg;C, stored under nitrogen, away from moisture
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (stored under nitrogen, away from moisture)
溶解性数据
In Vitro:;
DMSO : 110 mg/mL (230.31 mM; Need ultrasonic)
H2O : 83.33 mg/mL (174.47 mM; Need ultrasonic)
配制储备液
浓度溶剂体积质量
1 mg
5 mg
10 mg
1 mM
2.0937 mL
10.4686 mL
20.9371 mL
5 mM
0.4187 mL
2.0937 mL
4.1874 mL
10 mM
0.2094 mL
1.0469 mL
2.0937 mL
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (stored under nitrogen, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
In Vivo:
请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Gaudette NF, et al. Determination of methylene blue and leucomethylene blue in male and female Fischer 344 rat urine andB6C3F1 mouse urine. J Anal Toxicol. 2005 Jan-Feb;29(1):28-33.
[2]. Zhenhua Liu, et al. Amyloid β and tau are involved in sleep disorder in Alzheimer’s disease by orexin A and adenosine A(1) receptor. Int J Mol Med. 2019 Jan;43(1):435-442.
[3]. Francesco Panza, et al. Tau aggregation inhibitors: the future of Alzheimer’s pharmacotherapy? Expert Opin Pharmacother. 2016;17(4):457-61.
Bromothymol Blue 是一种 pH 指示剂，是测定弱酸弱碱时使用的一种指示剂，其酸性时为黄色，碱性时为蓝色。
Bromothymol Blue Chemical Structure
CAS No. : 76-59-5
规格
价格
是否有货
数量
Free Sample (0.1-0.5 mg)
;
Apply now
;
10;mM;*;1 mL in DMSO
￥440
In-stock
500 mg
￥400
In-stock
1 g
￥500
In-stock
5 g
;
询价
;
10 g
;
询价
;
* Please select Quantity before adding items.
Bromothymol Blue 相关产品
bull;相关化合物库:
Covalent Screening Library Plus
Bioactive Compound Library Plus
Covalent Screening Library
生物活性
Bromothymol Blue is a pH indicator.
体外研究 (In Vitro)
A first characterization and comparison is done by developing an easy and direct measurement method based on a pH indicator system using Bromothymol Blue (BTB) as the indicator and potassium phosphate as the buffer. A pH-shift assay is developed on the basis of BTB as the pH indicator and potassium phosphate as the buffer component[1]. Three pH indicators are tested for the direct determination of 2- 2-keto-L-gulonic acid (2-KLG) production on a plate. The results show that Bromothymol Blue is superior to the other two indicators in terms of the obvious color change and a suitable pH range (blue to yellow at pH 6.5-7.5). Upon the addition of a Bromothymol Blue solution (0.1%, w/v) to an agar plate, zones surrounding colonies of K. vulgare 07 mutants change their color from blue to yellow because K. vulgare 07 mutants release 2-KLG on agar plates, thereby acidifying surrounding areas around colonies[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
624.38
Formula
C27H28Br2O5S
CAS 号
76-59-5
中文名称
溴麝香草酚蓝；溴百里酚蓝；溴百里香酚蓝；溴百里香酚蓝；溴化麝香草酚蓝；溴瑞香草酚蓝
运输条件
Room temperature in continental US; may vary elsewhere.
[1]. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80.
[2]. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul – Sep;48(3):397-402.
Kinase Assay [1]
For all three enzymes, an expression in the 96-deep well scale is performed. Therefore, electrocompetent E. coli ArcticExpress(DE3) cells are transformed with the corresponding plasmid. Single clones are picked using the CP7200 Colony Picker and transferred to 96-deep well plates filled with 1.2 mL autoinduction media by a MicroFlo Select dispenser. After incubation (36 h, 37°C at 1,000 rpm), further processing is done manually. First, 100 μL of cell culture is transferred into a 96-well plate (U-shaped bottom) and harvested by centrifugation (4,570 rpm, 10 min at RT) while the supernatant is discarded. The frozen pellets (1 h at -20°C) are thawed at room temperature for one hour to improve cell lysis. Lysis is continued by the addition of 30 μL lysis buffer (3 h, 1,000 rpm, 37°C) containing 2 mM KPi, pH 7.0, 2 mM MgCl2, 10 μg/mL DNaseI, 100 μg/mL lysozyme. Next, 120 μL buffer (2 mM KPi, pH 7.0) is added followed by centrifugation (3,000 rpm, 15 min at RT). For the photometric measurement, 20 μL of the crude extract is transferred to a 96-well plate (F-shaped bottom) and the reaction is started by adding 180 μL master mix to give a final volume of 200 μL (2.5 mM KPi, pH 7.0, 2 mM MgCl2, 25 μg/mL BTB and 5 mM keto-deoxy-D-glucarate). The measurements are carried out for 60 min at 2-min intervals. Depending on the enzyme, different time windows are used for the activity calculation[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Pick A, et al. Identification and characterization of two new 5-keto-4-deoxy-D-Glucarate Dehydratases/Decarboxylases. BMC Biotechnol. 2016 Nov 17;16(1):80.
[2]. Yang W, et al. A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. Braz J Microbiol. 2017 Jul – Sep;48(3):397-402.
Isosulfan blue 是一种用于淋巴管造影中淋巴管识别的蓝色染料。Isosulfan blue 用于乳腺癌前哨淋巴结活检。Isosulfan blue 在乳腺癌手术中可能有过敏反应。
Isosulfan blue Chemical Structure
CAS No. : 68238-36-8
规格
价格
是否有货
数量
10;mM;*;1 mL in DMSO
￥4360
In-stock
5 mg
￥3500
In-stock
10 mg
￥5500
In-stock
50 mg
询价
100 mg
询价
* Please select Quantity before adding items.
Isosulfan blue 相关产品
bull;相关化合物库:
Drug Repurposing Compound Library Plus
FDA-Approved Drug Library Plus
Bioactive Compound Library Plus
FDA-Approved Drug Library
Anti-Cancer Compound Library
Drug Repurposing Compound Library
FDA Approved amp; Pharmacopeial Drug Library
Anti-Breast Cancer Compound Library
生物活性
Isosulfan blue is a blue dye for the identification of lymph vessels during lymphangiography. Isosulfan blueis is used during sentinel lymph node biopsies in breast cancer. Isosulfan blue is possible to have an allergic reaction during breast cancer operations[1][2].
体外研究 (In Vitro)
Isosulfan blue can rapidly uptake into lymph tissue with little diffusion to other surrounding tissues[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial
分子量
566.66
Formula
C27H31N2NaO6S2
CAS 号
68238-36-8
运输条件
Room temperature in continental US; may vary elsewhere.
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Efron P, et al. Anaphylactic reaction to isosulfan blue used for sentinel node biopsy: case report and literature review. Breast J. 2002 Nov-Dec;8(6):396-9.
[2]. Laurie SA, et al. Anaphylaxis to isosulfan blue. Ann Allergy Asthma Immunol. 2002 Jan;88(1):64-6.
