5B/110mm-Advantec中速无灰级5B定量滤纸

  • 型号 5B/110mm
  • 品牌 ADVANTEC东洋
  • 【简单介绍】

    Advantec中速无灰级5B定量滤纸,尺寸(公分/直径):5.5,7.0,9.0,11.0,12.5,15.0,18.5,24.0,30.0,40.0,50.0,60.0,100张/盒。
    可依需要订购其他尺寸规格。

    Advantec中速无灰级5B定量滤纸 5B/110mm

    日本ADVANTEC 5B定量滤纸FILTER PAPER QUANTITATIVE ASHLESS   ADVANTEC  5C   100 CIRCLES   110mm:日本TOYO ADVANTEC定量滤纸,主要用途有土壤试验、肥料试验、矿物试验、电解分析、比色分析、药学、动植物学、***试验、火yao实验、医学实验、微量金属分析、饮料水分析、沉淀物过滤等。定量滤纸灰分0.01%(其中硬质滤纸No.4A为0.025%),能耐120℃高温,pH范围0~12(其中硬质滤纸No.4A为0~14)。包装100片/盒。有各种尺寸可供选择:φ55mm、φ70mm、φ90mm、φ110mm、φ125mm、φ150mm、φ185mm、φ240mm、φ285mm、φ300mm、φ330mm、φ360mm、φ400mm、φ500mm、485x560mm。

     日本TOYO ADVANTEC产品。日本ADVANTEC定量滤纸No.5B 

    编号 重量g/cm 厚度mm 过滤时间sec 吸水高度cm 湿润破裂强度kPa 保留粒径 

    No.3 113 0.23 130 7.5 1.9 5μm 

    No.4A 96 0.12 915 4.0 8.8 1μm 

    No.5A 97 0.22 60 9.5 1.4 7μm 

    No.5B 108 0.21 195 7.0 1.9 4μm 

    No.5C 118 0.22 570 6.0 2.4 1μm 

    No.6 103 0.20 300 6.0 1.4 3μm 

    No.7 87 0.18 200 7.0 1.1 4μm 

    1.过滤速度:于20℃时,以10cm水银柱压力,过滤100ml之蒸馏水所需的时间。 

    2.吸水速度:于20℃时,一垂直条状过滤纸,10分钟内水由下往上吸附移动距离。 

    3.破裂强度:10cm润湿的滤纸所能承受之水柱压力值。 

    用途: 

    No.3:过滤速度较快:工业QA/QC定量分析,肥料分析、土壤沉淀物、矿物分析、水泥分析。 

    No.4A:硬质滤纸,耐压力及酸碱:过滤细微之结晶沉淀物。 

    No.5A:过滤速度zui快,粗粒子过滤:沉淀物过滤、电解液用比色样品前过滤、环境污染监测取样 

    No.5B:过滤速度中等:一般定量分析之沉淀过滤。 

    No.5C:细粒子过滤:沉淀物过滤及沉淀物有collodical dispersion倾向的溶液。 

    No.6:不溶解的沉淀物过滤:过量稀有金属分析、饮料及火yao分析。 

    No.7:精密定量分析:有机物及动植物样品中银,水银分析,微量稀有金属分析。 

    尺寸(公分/直径):5.5,7.0,9.0,11.0,12.5,15.0,18.5,24.0,30.0,40.0,50.0,60.0,100张/盒。 

    可依需要订购其他尺寸规格。

    Advantec中速无灰级5B定量滤纸 5B/110mm

    订货信息:

    5B/110mm-Advantec中速无灰级5B定量滤纸

     

ESCOLabculture®B2型二级生物安全柜

ESCOLabculture®B2型二级生物安全柜

ESCO Labculture® B2型二级生物安全柜

产品编号:
市场价:¥0.00
会员价:¥0.00
品牌:ESCO
生产厂家:ESCO
需求数量:

  • 产品概览
  • 技术参数
  • 订购信息
  • 相关资料
  • 相关产品

商品描述:

应用领域:
生物安全等级P1~P3级的样品,允许样品含有挥发性有毒化学物质或化学气体,应用于高危险微生物以及有害物质。

主要特点:
◆获得中国医疗器械注册证(注册号:国食药监械(进)字2006第3541961号)
◆100%全排放式,所有气体不重复循环使用,污染空气直接经过ULPA滤膜过滤以后,在外接风机和外排管道作用下,排放到室外环境
◆ULPA超高效过滤器,针对0.12um颗粒系可以达到99.9999%截流效率;
◆高品质高风量风机,自动风量风压平衡补偿,低能耗,低噪音;
◆背板及侧壁一体圆弧成型无死角,背部、顶端以及管道包围式负压腔设计,使得操作室排出的污染气体完全处于负压环境包围之下,避免了有毒有害气体泄漏的可能性;
◆人体工程学10度倾斜角设计,增加操作舒适性;
◆底部台面不锈钢一体化设计;
◆安全柜的各项气流数值可在液晶控制屏上实时显示,并拥有风速自动补偿功能;
◆安全柜装配功能完善的Sentinel微电脑控制系统,操作人员可根据实际要求自主设定各项参数;
◆Sentinel控制器拥有最新的智能硬件诊断系统,可即时对安全柜操作系统进行检测并提供错误诊断报告;
◆灵敏的声光警报系统可监控安全柜运行的各项参数,一旦出现任何不安全的状况,例如前窗处于非安全高度或者风速过低,系统都将给予声/光警报;
◆安全柜控制系统将紫外线杀菌灯与前窗位置进行互锁;
◆ULPA过滤器和UV紫外灯的使用寿命显示;
◆采用Isocide抗菌涂层可抑制细菌在柜体表面滋生;

技术参数与订货信息

型号/货号
技术参数
LB23B1
LB24B1
LB25B1
LB26B1
外部尺寸(L x W x H mm)
1035 x 811 x 1460
1340 x 811 x 1460
1645 x 811 x 1460
1950 x 811 x 1460
内部尺寸(L x W x H mm)
965 x 585 x 670
1270 x 585 x 670
1575 x585 x 670
1880 x 585 x 670
产品执行标准
美国:NSF49认证; 中国食品药品监督管理局的型式检测;
过滤器性能标准:IEST-RP-CC034.1,IEST-RP-CC007和EN1822(H14);
电气安全标准:IEC61010-1/EN61010-1/ UL6101-1/CAN/CSA C22.2 No.61010.1-1
平均气流风速
进气气流
起始设定值:0.53米/秒(当气流速度低于0.4m/s时,声光报警启动)
沉降气流
起始设定值:0.35米/秒(当气流波动超过标称值20%时,声光报警启动)
气体交换体积
进气气流
376立方米/小时
492立方米/小时
608立方米/小时
724立方米/小时
沉降气流
709立方米/小时
928立方米/小时
1147立方米/小时
1367立方米/小时
排出气流
1085立方米/小时
1420立方米/小时
1755立方米/小时
2091立方米/小时
工作区内空气洁净度标准
ISO14644.1 Class 3,美国联邦标准209E Class 1/M1.5,AS1386 Class1.5,JIS B9920 Class 3,BS5295 Class C等世界各国同类标准
ULPA下降气流过滤器截流效率
>99.999%,针对>0.12微米颗粒系
HEPA外排气流过滤器截流效率
>99.99%,针对>0.3微米颗粒系
噪音标准(根据 EN124699标准)
<55dBA
<56dBA
<57dBA
<58dBA
光照强度(根据 NSF 49标准)
>1250Lux
>1400Lux
>1200Lux
>1200Lux
主体结构
1.5mm厚镀锌钢板,外加Isocide抗菌涂层
侧壁结构
1.2mm厚304#不锈钢侧壁,双层负压腔设计
电源/电压
220-240V/50Hz,单向
净重kg
279
317
359
438

 配件信息:

订货号
技术参数
SPC-XA0
711mm支架(x为柜体尺寸,例SPC-4A0)
UV-15A
15w,254nm紫外灯,适用于3尺柜体
UV-30A
30w,254nm紫外灯,适用于4尺以上柜体
EO-HE
防溅电源插座
SF-1W
欧式水接口
SF-2U
美式通用型气体接口

5B/55mm-ADVANTEC中速5B定量滤纸直径55mm

  • 型号 5B/55mm
  • 品牌 ADVANTEC东洋
  • 【简单介绍】

    ADVANTEC中速5B定量滤纸直径55mm
    .吸水速度:于20℃时,一垂直条状过滤纸,10分钟内水由下往上吸附移动距离。
    .破裂强度:10cm润湿的滤纸所能承受之水柱压力值。
    .过滤速度:于20℃时,以10cm水银柱压力,过滤100ml之蒸馏水所需的时间。

    ADVANTEC 5B定量滤纸(FILTER PAPER QUANTITATIVE ASHLESS  ADVANTE 5B):ADVANTEC 5B 滤纸 FILTER PAPER过滤速度中等:一般定量分析之沉淀过滤。5B 110MM 108 g/cm,0.21 mm,195 sec,7 cm,1.9 kPa,4µm

    日本TOYO ADVANTEC定量滤纸,ADVANTEC 5B定量滤纸主要用途有土壤试验、肥料试验、矿物试验、电解分析、比色分析、药学、动植物学、**试验、火yao实验、医学实验、微量金属分析、饮料水分析、沉淀物过滤等。定量滤纸灰分0.01%(其中硬质滤纸No.4A为0.025%),能耐120℃高温,pH范围0~12(其中硬质滤纸No.4A为0~14)。包装100片/盒。有各种尺寸可供选择:φ55mm、φ70mm、φ90mm、φ110mm、φ125mm、φ150mm、φ185mm、φ240mm、φ285mm、φ300mm、φ330mm、φ360mm、φ400mm、φ500mm、485x560mm。

     

    编号 重量g/cm 厚度mm 过滤时间sec 吸水高度cm 湿润破裂强度kPa 保留粒径 
    No.3 113 0.23 130 7.5 1.9 5μm 
    No.4A 96 0.12 915 4.0 8.8 1μm 
    No.5A 97 0.22 60 9.5 1.4 7μm 
    No.5B 108 0.21 195 7.0 1.9 4μm 
    No.5C 118 0.22 570 6.0 2.4 1μm 
    No.6 103 0.20 300 6.0 1.4 3μm 
    No.7 87 0.18 200 7.0 1.1 4μm 

    1.过滤速度:于20℃时,以10cm水银柱压力,过滤100ml之蒸馏水所需的时间。 
    2.吸水速度:于20℃时,一垂直条状过滤纸,10分钟内水由下往上吸附移动距离。 
    3.破裂强度:10cm润湿的滤纸所能承受之水柱压力值。 
    ADVANTEC 5A定量滤纸用途: 
    No.3:过滤速度较快:工业QA/QC定量分析,肥料分析、土壤沉淀物、矿物分析、水泥分析。 
    No.4A:硬质滤纸,耐压力及酸碱:过滤细微之结晶沉淀物。 
    No.5A:过滤速度z快,粗粒子过滤:沉淀物过滤、电解液用比色样品前过滤、环境污染监测取样 
    No.5B:过滤速度中等:一般定量分析之沉淀过滤。 
    No.5C:细粒子过滤:沉淀物过滤及沉淀物有collodical dispersion倾向的溶液。 
    No.6:不溶解的沉淀物过滤:过量稀有金属分析、饮料及火yao分析。 
    No.7:精密定量分析:有机物及动植物样品中银,水银分析,微量稀有金属分析。 

    ADVANTEC中速5B定量滤纸直径55mm

    订货信息:

    5B/55mm-ADVANTEC中速5B定量滤纸直径55mm

Procyanidin B2(Synonyms: 原花青素 B2; Proanthocyanidin B2)

天然产物 黄酮类 Flavonoids

Procyanidin B2 (Synonyms: 原花青素 B2; Proanthocyanidin B2) 纯度: 99.45%

Procyanidin B2 是一种天然黄酮类物质,具有抗肿瘤,抗氧化等作用。

Procyanidin B2(Synonyms: 原花青素 B2; Proanthocyanidin B2)

Procyanidin B2 Chemical Structure

CAS No. : 29106-49-8

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥1485 In-stock
1 mg ¥430 In-stock
5 mg ¥1350 In-stock
10 mg ¥2300 In-stock
25 mg ¥4500 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Procyanidin B2 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Metabolism/Protease Compound Library
  • NF-κB Signaling Compound Library
  • Stem Cell Signaling Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Anti-Aging Compound Library
  • Antioxidants Compound Library
  • Oxygen Sensing Compound Library
  • Ferroptosis Compound Library
  • Phenols Library
  • Pyroptosis Compound Library
  • Traditional Chinese Medicine Monomer Library
  • Flavonoids Library
  • Mitochondria-Targeted Compound Library
  • Food-Sourced Compound Library

生物活性

Procyanidin B2 is a natural flavonoid, with anti-cancer, antioxidant activities.

体外研究
(In Vitro)

Procyanidin B2 shows antiproliferative activity to MCF-7 cells, with an IC50 of 19.21 μM. However, Procyanidin B2 exhibits no effect on DNA-ladder formation[1]. Procyanidin B2 (0.1, 1, 2 μM) inhibits the activation of pyrin domain containing 3 (NLRP3) inflammasome in human umbilical vein ECs (HUVECs), and the inhibition is via suppression of AP-1 activity, and such effect can be abolished by overexpression of c-Jun. Procyanidin B2 (2 μM for 12 h) also reduces ROS in HUVECs[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Procyanidin B2 (40, 20, and 10 mg/kg, p.o.) protects against cerebral ischemia-induced infarct volume and brain edema in rats. Procyanidin B2 (40 mg/kg, p.o) also improves functional outcomes, regulates blood-brain barrier (BBB) permeability after cerebral ischemia. Moreover, Procyanidin B2 attenuates cerebral ischemia-induced tight junction degradation, mitochondrial depolarization and intracellular oxidative stress. Procyanidin B2 (40 mg/kg, p.o) increases Nrf2 activation and HO-1, GSTα, and NQO1 protein expression in normal brains in vivo[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

578.52

Formula

C30H26O12

CAS 号

29106-49-8

中文名称

原花青素 B2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

H2O : 66.67 mg/mL (115.24 mM; Need ultrasonic)

DMSO : ≥ 50 mg/mL (86.43 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7285 mL 8.6427 mL 17.2855 mL
5 mM 0.3457 mL 1.7285 mL 3.4571 mL
10 mM 0.1729 mL 0.8643 mL 1.7285 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.32 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Avelar MM, et al. Procyanidin b2 cytotoxicity to mcf-7 human breast adenocarcinoma cells. Indian J Pharm Sci. 2012 Jul;74(4):351-5.

    [2]. Yang H, et al. Procyanidin B2 inhibits NLRP3 inflammasome activation in human vascular endothelial cells. Biochem Pharmacol. 2014 Dec 15;92(4):599-606.

    [3]. Wu S, et al. Procyanidin B2 attenuates neurological deficits and blood-brain barrier disruption in a rat model of cerebral ischemia. Mol Nutr Food Res. 2015 Oct;59(10):1930-41.

Cell Assay
[1]

MCF-7 cells are grown in 5 mL of RPMI 1640 medium supplemented with 20% fetal bovine serum, penicillin (100 U/mL) and streptomycin (100 μg/mL). Cells are maintained at 37°C in a humidified atmosphere of 5% CO2 in air and subcultured every 3 days. Exponentially growing cells are plated at a seeding density of 2×104 cells/mL, in 96-well plates or in dishes. After overnight incubation to allow for attachment, the cells are exposed for 24 or 48 h to various concentrations of Procyanidin B2 (0.5; 1.0; 5.0; 10.0; 25.0 and 50.0 μM) diluted in distilled water. Two millimolar cyclophosphamide (CP) is used as the positive control and solutions are sterilised by filtration[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

To observe the effect of Procyanidin B2 on infarct size or brain edema, 40 rats are randomLy separated into five groups, and each group is administered with vehicle (equivalent dose of 0.9% saline administered via gavage) or 40, 20, or 10 mg/kg of Procyanidin B2, respectively. To elucidate the effect of Procyanidin B2 on blood-brain barrier (BBB) permeability, the rats (n = 6 per group for Evans blue extravasation and n = 4 per group for IgG leakage) are randomLy separated into two groups: vehicle and Procyanidin B2 (40 mg/kg). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after middle cerebral artery occlusion (MCAO). For other observations, including immunohistological staining and western blot analysis, rats are randomLy separated into three groups: sham-operated, vehicle, and Procyanidin B2 (n = 4 per group). Procyanidin B2 (40 mg/kg) is administered intragastrically once a day starting at 3 h after MCAO. To elucidate the improvement in neurological function after ischemic stroke in rats, the rats (n = 8 per group) undergo neurobehavioral assays to evaluate the functional outcome after administration of Procyanidin B2 (40 mg/kg) once a day starting at 24 h after MCAO. The neurological deficits are assessed at 1, 3, 7, 11, and 14 days after MCAO[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Avelar MM, et al. Procyanidin b2 cytotoxicity to mcf-7 human breast adenocarcinoma cells. Indian J Pharm Sci. 2012 Jul;74(4):351-5.

    [2]. Yang H, et al. Procyanidin B2 inhibits NLRP3 inflammasome activation in human vascular endothelial cells. Biochem Pharmacol. 2014 Dec 15;92(4):599-606.

    [3]. Wu S, et al. Procyanidin B2 attenuates neurological deficits and blood-brain barrier disruption in a rat model of cerebral ischemia. Mol Nutr Food Res. 2015 Oct;59(10):1930-41.

荧光染料Br-Mmc(Synonyms: 4-Bromomethyl-7-methoxycoumarin)

荧光染料Dye Reagents Br-Mmc;(Synonyms: 4-Bromomethyl-7-methoxycoumarin)

Br-Mmc (4-Bromomethyl-7-methoxycoumarin) 通常用作荧光标记,用于测定含有羧基的化合物。Br-Mmc 可通过 TLC 或 HPLC 测定脂肪酸含量。

Br-Mmc(Synonyms: 4-Bromomethyl-7-methoxycoumarin)

Br-Mmc Chemical Structure

CAS No. : 35231-44-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Br-Mmc 相关产品

bull;相关化合物库:

  • Fragment Library

生物活性

Br-Mmc (4-Bromomethyl-7-methoxycoumarin) is often used as fluorescent label for the determination of compounds possessing a carboxylic group. Br-Mmc is used for the determination fatty acids by TLC or HPLC[1][2][3].

分子量

269.09

Formula

C11H9BrO3

CAS 号

35231-44-8

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
参考文献
  • [1]. Wolf JH, et al. 4-Bromomethyl-7-methoxycoumarin and analogues as derivatization agents for high-performance liquid chromatography determinations: a review. J Pharm Biomed Anal. 1992 Feb-Mar;10(2-3):99-107.

    [2]. Wang K, et al. Derivatization of 5-fluorouracil with 4-bromomethyl-7-methoxycoumarin for determination by liquid chromatography-mass spectrometry. J Am Soc Mass Spectrom. 1998 Sep;9(9):970-6.

    [3]. Hagen V, et al. Synthesis, photochemistry and application of (7-methoxycoumarin-4-yl)methyl-caged 8-bromoadenosine cyclic 3′,5′-monophosphate and 8-bromoguanosine cyclic 3′,5′-monophosphate photolyzed in the nanosecond time region. J Photochem Photobiol B. 1999 Nov-Dec;53(1-3):91-102.

Procyanidin B1(Synonyms: 原花青素 B1)

天然产物 黄酮类 Flavonoids

Procyanidin B1 (Synonyms: 原花青素 B1) 纯度: 99.28%

Procyanidin B1 是一种多酚类黄酮物质,存在于常见的水果中,能够与 TLR4/MD-2 复合体结合,具有抗炎活性。

Procyanidin B1(Synonyms: 原花青素 B1)

Procyanidin B1 Chemical Structure

CAS No. : 20315-25-7

规格 价格 是否有货 数量
1 mg ¥2200 In-stock
5 mg ¥6500 In-stock
10 mg ¥9500 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Procyanidin B1 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus

生物活性

Procyanidin B1 is a polyphenolic flavonoid isolated from commonly eaten fruits, binds to TLR4/MD-2 complex, and has anti-inflammatory activity.

体外研究
(In Vitro)

Procyanidin B1 is a polyphenolic flavonoid isolated from fruits and fruit juices, binds to TLR4/MD-2 complex, and has anti-inflammatory activity. Procyanidin B1 causes cellular toxicity at concentrations >100 μg/mL. Procyanidin B1 (100 μg/mL) inhibits LPS-induced TNF-α production, and expression of MD-2, TRAF6, NF-κB mRNA, phosphorylated p38 MAPK and NF-κB protein in THP1 cells[1]. Procyanidin B1 (50-100 µM) protects against Aβ oligomer-induced neuronal death. Procyanidin B1 potently inhibits the activation of caspase-3 at 100 µM, caspase-8 at concentrations of 30, 50, and 100 µM and caspase-9 at concentrations of 10, 30, 50, and 100 µM[2]. Procyanidin B1 (10, 20, 30 μM) significantly and dose-dependently induces expression of ACO and CPT1, with no obvious effect on mRNA expression of PPARα[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

578.52

Formula

C30H26O12

CAS 号

20315-25-7

中文名称

原花青素 B1

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 25 mg/mL (43.21 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7285 mL 8.6427 mL 17.2855 mL
5 mM 0.3457 mL 1.7285 mL 3.4571 mL
10 mM 0.1729 mL 0.8643 mL 1.7285 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Xing J, et al. Anti-inflammatory effect of procyanidin B1 on LPS-treated THP1 cells via interaction with the TLR4-MD-2 heterodimer and p38 MAPK and NF-κB signaling. Mol Cell Biochem. 2015 Sep;407(1-2):89-95.

    [2]. Kanno H, et al. Protective effects of glycycoumarin and procyanidin B1, active components of traditional Japanese medicine yokukansan, on amyloid β oligomer-induced neuronal death. J Ethnopharmacol. 2015 Jan 15;159:122-8.

    [3]. Shimada T, et al. Flavangenol (pine bark extract) and its major component procyanidin B1 enhance fatty acid oxidation in fat-loaded models. Eur J Pharmacol. 2012 Feb 29;677(1-3):147-53.

Cell Assay
[1]

To investigate the cytotoxic effect of Procyanidin B1, viability of THP1 cells is assessed using CCK8 assay. THP1 cells are treated with Procyanidin B1 for 18 h, and 10 μL of CCK8 solution is then added to each well and the cultures are incubated for 4 h at 37°C. The optical density (OD) at 450 nm is measured using an ELx808 Absorbance Microplate Reader. The Procyanidin B1 concentration tested ranges from 50 to 200 μg/mL. Each sample is tested in triplicate[1].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Xing J, et al. Anti-inflammatory effect of procyanidin B1 on LPS-treated THP1 cells via interaction with the TLR4-MD-2 heterodimer and p38 MAPK and NF-κB signaling. Mol Cell Biochem. 2015 Sep;407(1-2):89-95.

    [2]. Kanno H, et al. Protective effects of glycycoumarin and procyanidin B1, active components of traditional Japanese medicine yokukansan, on amyloid β oligomer-induced neuronal death. J Ethnopharmacol. 2015 Jan 15;159:122-8.

    [3]. Shimada T, et al. Flavangenol (pine bark extract) and its major component procyanidin B1 enhance fatty acid oxidation in fat-loaded models. Eur J Pharmacol. 2012 Feb 29;677(1-3):147-53.

L-Carnosine

L-Carnosine; 纯度: 99.94%

L-Carnosine 是 beta-丙氨酸和组氨酸组成的二肽,能够抑制许多衰老带来的生化变化。

L-Carnosineamp;;

L-Carnosine Chemical Structure

CAS No. : 305-84-0

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in Water ¥550 In-stock
100 mg ¥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

L-Carnosine 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Drug Repurposing Compound Library Plus
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus
  • Metabolism/Protease Compound Library
  • Natural Product Library
  • Clinical Compound Library
  • Human Endogenous Metabolite Compound Library
  • Drug Repurposing Compound Library
  • Anti-COVID-19 Compound Library
  • Alkaloids Library
  • Peptide Library

生物活性

L-Carnosine is a dipeptide of the amino acids beta-alanine and histidine and has the potential to suppress many of the biochemical changes that accompany aging.

IC50 Target

Human Endogenous Metabolite

;

体外研究
(In Vitro)

L-Carnosine is a dipeptide of the amino acids beta-alanine and histidine and has the potential to suppress many of the biochemical changes that accompany aging[1]. Carnosine also exhibits some antioxidant effects. The antioxidant mechanism of carnosine is attributed to its chelating effect against metal ions, superoxide dismutase (SOD)-like activity, and ROS and free radicals scavenging ability[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

226.23

Formula

C9H14N4O3

CAS 号

305-84-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : 83.33 mg/mL (368.34 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 4.4203 mL 22.1014 mL 44.2028 mL
5 mM 0.8841 mL 4.4203 mL 8.8406 mL
10 mM 0.4420 mL 2.2101 mL 4.4203 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Hipkiss AR, et al. Would carnosine or a carnivorous diet help suppress aging and associated pathologies? Ann N Y Acad Sci. 2006 May;1067:369-74.

    [2]. Guney Y, et al. Carnosine may reduce lung injury caused by radiation therapy. Med Hypotheses. 2006;66(5):957-9.

Ginsenoside Re(Synonyms: 人参皂苷 Re; Ginsenoside B2; Panaxoside Re; Sanchinoside Re)

天然产物 糖类和糖苷 Saccharides and Glycosides

Ginsenoside Re;(Synonyms: 人参皂苷 Re; Ginsenoside B2; Panaxoside Re; Sanchinoside Re) 纯度: 98.15%

Ginsenoside Re (Ginsenoside B2) 是一种 Panax notoginseng 提取物。Ginsenoside Re 可降低 β-淀粉样蛋白 ()。Ginsenoside Re 还通过抑制 JNKNF-κB 发挥抗炎作用。

Ginsenoside Re(Synonyms: 人参皂苷 Re; Ginsenoside B2;  Panaxoside Re;  Sanchinoside Re)

Ginsenoside Re Chemical Structure

CAS No. : 52286-59-6

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ¥886 In-stock
5 mg ¥550 In-stock
10 mg ¥850 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Ginsenoside Re 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Kinase Inhibitor Library
  • MAPK Compound Library
  • Metabolism/Protease Compound Library
  • Neuronal Signaling Compound Library
  • NF-kappa;B Signaling Compound Library
  • Stem Cell Signaling Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Clinical Compound Library
  • Human Endogenous Metabolite Compound Library
  • Anti-Aging Compound Library
  • Antioxidants Compound Library
  • Differentiation Inducing Compound Library
  • Reprogramming Compound Library
  • Glycoside Compound Library
  • Lipid Compound Library
  • Oxygen Sensing Compound Library
  • Medicine Food Homology Compound Library
  • Pyroptosis Compound Library
  • Traditional Chinese Medicine Monomer Library
  • FDA Approved amp; Pharmacopeial Drug Library
  • Anti-Alzheimer’s Disease Compound Library
  • Anti-Breast Cancer Compound Library
  • Anti-Pancreatic Cancer Compound Library
  • Anti-Blood Cancer Compound Library
  • Anti-Parkinson’s Disease Compound Library
  • Neurodegenerative Disease-related Compound Library
  • Anti-Obesity Compound Library
  • Transcription Factor Targeted Library
  • Food-Sourced Compound Library
  • Anti-Liver Cancer Compound Library

生物活性

Ginsenoside Re (Ginsenoside B2) is an extract from Panax notoginseng. Ginsenoside Re decreases the β-amyloid protein (). Ginsenoside Re plays a role in antiinflammation through inhibition of JNK and NF-κB.

IC50 Target[1][2]

1-40

;

1-42

;

NF-κB

;

JNK

;

Human Endogenous Metabolite

;

体外研究
(In Vitro)

Ginsenoside Re is a well-known traditional Chinese medicine, which decreases the β-site amyloid precursor protein cleaving enzyme 1 (BACE1) mRNA and protein levels and inhibits BACE1 activity in the N2a/APP695 cells. Ginsenoside Re also significantly increases the PPARγ protein and mRNA levels.To prevent Ginsenoside Re from having a cytotoxic effect on the N2a/APP695 cells, the cell viability is first determined by the MTT assay. The N2a/WT and N2a/APP695 cells are treated with increasing concentrations of Ginsenoside Re (0-200 µM) for 24 h. Ginsenoside Re concentrations under 100 µM do not affect the viability of the N2a/WT and N2a/APP695 cells, whereas the 150 µM Ginsenoside Re concentration markedly decreases the survival rate of the N2a/WT and N2a/APP695 cells. Incubation with Ginsenoside Re at a 200 µM concentration for 24 h reduces the viability of the N2a/WT and N2a/APP695 cells by 15.58% and 26.82%, respectively. These data indicate that Ginsenoside Re treatment within the range of 0-100 µM for 24 h is safe for the N2a/WT and N2a/APP695 cells (P>0.05)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Ginsenoside Re reduces insulin resistance in 3T3-L1 adipocytes and high-fat diet (HFD) rats through inhibition of JNK and NF-κB activation[2]. Intraperitoneal injection of lipopolysaccharide (LPS) at a dose of 20 mg/kg is lethal to mice, and 70% to 80% of the mice die within 60 h. However, pretreatment of the mice with Rg1 or Ginsenoside Re increases their survival rates in a dose-dependent manner. With the doses of Rg1 or Ginsenoside Re increase from 2.5 to 5 mg/kg, the survival rate is elevated from 60% to 90% (Rg1) or from 30% to 40% (Ginsenoside Re). All the mice administered Rg1 at a minimal dose of 10 mg/kg are protected from death compared to 80% survival of mice treated with an equal dose of Ginsenoside Re. To protect all the mice, 20 mg/kg Ginsenoside Re is needed. To investigate the anti-inflammatory potential of Rg1 and Ginsenoside Re, 1 mg/kg Rg1 or Ginsenoside Re is injected into rats and then challenged the animals with LPS. The injection procedure itself causes a transient stress-induced increase in body temperature of ~1.2°C in each group. Thereafter, LPS-challenged rats without pretreatment develope a robust biphasic fever, with the first peak reaching ~1.5°C at 2 h and the second peak reaching 1.8°C at 4 h. In contrast, the temperature changes for the Rg1-, Ginsenoside Re-, and TAK-242-treated groups are only 0.9, 1.2, and 0.8°C at 2 h and 1.3, 1.4, and 1.0°C at 4 h, respectively. Pretreatment with Rg1, Ginsenoside Re, or TAK-242 significantly attenuates LPS-induced alterations in body temperature[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

947.15

Formula

C48H82O18

CAS 号

52286-59-6

中文名称

人参皂苷 Re

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : ≥ 50 mg/mL (52.79 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.0558 mL 5.2790 mL 10.5580 mL
5 mM 0.2112 mL 1.0558 mL 2.1116 mL
10 mM 0.1056 mL 0.5279 mL 1.0558 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (2.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (2.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.64 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.64 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.64 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Cao G, et al. Ginsenoside Re reduces Aβ production by activating PPARγ to inhibit BACE1 in N2a/APP695 cells. Eur J Pharmacol. 2016 Dec 15;793:101-108.

    [2]. Su F, et al. Protective effect of ginsenosides Rg1 and Re on lipopolysaccharide-induced sepsis by competitive binding to Toll-like receptor 4. Antimicrob Agents Chemother. 2015 Sep;59(9):5654-63.

    [3]. Zhang Z, et al. Ginsenoside Re reduces insulin resistance through inhibition of c-Jun NH2-terminal kinase and nuclear factor-kappaB. Mol Endocrinol. 2008 Jan;22(1):186-95.

Cell Assay
[1]

To explore the cytotoxic effect of Ginsenoside Re on N2a/APP695 cells, cell proliferation is assessed using the MTT assay. The cells are treated with increasing doses of Ginsenoside Re (0, 25, 50, 100, 150, and 200 µM) for 24 h. The MTT assay is performed after the treatments. The cells are incubated for 4 h at 37 °C with 0.5 mg/mL of MTT dissolved in fresh complete medium. The dark blue formazan crystals are dissolved in DMSO, and the absorbance is measured on a microplate reader using a reference wavelength of 630 nm and a test wavelength of 490 nm. The data are expressed as the mean percentages of viable cells versus the control[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration
[3]

Mice[3]
To examine the prophylactic effect of Rg1 and Ginsenoside Re on LPS-induced lethality, 6- to 8-week-old BALB/c mice are randomly assigned to 7 groups with 10 mice in each group. The mice are either left untreated or subcutaneously injected with 2.5, 5, 10, or 20 mg/kg Rg1, 20 mg/kg Ginsenoside Re, or 5 mg/kg TAK-242 3 times at 30-min intervals and then challenged with LPS (20 mg/kg) 15 min later. To test the therapeutic effect of Rg1 and Ginsenoside Re on LPS-induced lethality, BALB/c mice are assigned to 5 groups with 10 mice in each group. The mice are injected intraperitoneally (i.p.) with 20 mg/kg LPS. Fifteen minutes later, mice are subcutaneously injected with 10 mg/kg Rg1, 20 mg/kg Ginsenoside Re, or 5 mg/kg TAK-242 3 times at 30-min intervals or left untreated. Survival rates are recorded for 60 h.
Rats[3]
Sprague Dawley rats are intravenously administered saline solution, 1 mg of Rg1/kg body weight (1 mg/kg Rg1), 1 mg/kg Ginsenoside Re, or 1 mg/kg TAK-242. Fifteen minutes later, rats are challenged with 2.5 μg/kg LPS. Body temperature is measured before and after drug administration. Anticoagulated blood samples with EDTA are collected for white blood cell (WBC) counts at indicated time points using a ProCyte Dx automatic blood cell analyzer. Additional blood samples are collected at 4 h post-drug injection and used for preparation of serum to analyze proinflammatory mediators. The proinflammatory cytokine responses are detected by Western blot assay.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Cao G, et al. Ginsenoside Re reduces Aβ production by activating PPARγ to inhibit BACE1 in N2a/APP695 cells. Eur J Pharmacol. 2016 Dec 15;793:101-108.

    [2]. Su F, et al. Protective effect of ginsenosides Rg1 and Re on lipopolysaccharide-induced sepsis by competitive binding to Toll-like receptor 4. Antimicrob Agents Chemother. 2015 Sep;59(9):5654-63.

    [3]. Zhang Z, et al. Ginsenoside Re reduces insulin resistance through inhibition of c-Jun NH2-terminal kinase and nuclear factor-kappaB. Mol Endocrinol. 2008 Jan;22(1):186-95.

Pulchinenoside C(Synonyms: Anemoside B4)

天然产物 糖类和糖苷 Saccharides and Glycosides

Pulchinenoside C;(Synonyms: Anemoside B4) 纯度: ge;98.0%

Pulchinenoside C (Anemoside B4) 是一个天然的白头翁皂苷B4,有很多生物效应,例如抗肿瘤,神经保护,抗血管生成等活性。

Pulchinenoside C(Synonyms: Anemoside B4)

Pulchinenoside C Chemical Structure

CAS No. : 129741-57-7

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1612 In-stock
5 mg ¥700 In-stock
10 mg ¥1200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Pulchinenoside C 相关产品

bull;相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Diabetes Related Compound Library
  • Glycoside Compound Library
  • Terpenoids Library
  • Traditional Chinese Medicine Monomer Library
  • Neuroprotective Compound Library

生物活性

Pulchinenoside C (Anemoside B4) is Pulsatilla koreana Nakai that have many numerous biological effects in vitro, including enhancing hypoglycemic, anti-tumor, neuroprotective and anti-angiogenic activity.

体外研究
(In Vitro)

Pulchinenoside C (Anemoside B4) can inhibit the secretion of IL-10; SSA, SSD and PNS up-regulated IL-2 expression[1]. Pulchinenoside C (Anemoside B4), with IC50 value more than 390 ug/mL. Anemoside B4 inhibits cell proliferation[2]. Pulchinenoside C (Anemoside B4) can significantly suppress the secretion of the inflammatory factor E-selectin by endothelial cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1221.38

Formula

C59H96O26

CAS 号

129741-57-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 125 mg/mL (102.34 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8187 mL 4.0937 mL 8.1875 mL
5 mM 0.1637 mL 0.8187 mL 1.6375 mL
10 mM 0.0819 mL 0.4094 mL 0.8187 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (1.70 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (1.70 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (1.70 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (1.70 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Hu Y et al. Role of Chinese herbal medicinal ingredients in secretion of cytokines by PCV2-induced endothelial cells. J Immunotoxicol. 2016;13(2):141-7.

    [2]. Liu M et al. Cytotoxicity of the compounds isolated from Pulsatilla chinensis saponins and apoptosis induced by 23-hydroxybetulinic acid. Pharm Biol. 2015 Jan;53(1):1-9.

    [3]. Hu Y et al. Chinese herbal medicinal ingredients inhibit secretion of IL-6, IL-8, E-selectin and TXB2 in LPS-induced rat intestinal microvascular endothelial cells. Immunopharmacol Immunotoxicol. 2009;31(4):550-5.

Procyanidin B3(Synonyms: 原花青素 B3)

天然产物 黄酮类 Flavonoids

Procyanidin B3 (Synonyms: 原花青素 B3) 纯度: 99.63%

Procyanidin B3 是一种天然产物,为组蛋白乙酰转移酶 (HAT) 特异性抑制剂,可与 p300 的其他位点而非活性位点结合,选择性地抑制 p300 介导的 androgen 受体乙酰化。Procyanidin B3 对 HDAC 或 组蛋白甲基转移酶 (HMT) 无作用。

Procyanidin B3(Synonyms: 原花青素 B3)

Procyanidin B3 Chemical Structure

CAS No. : 23567-23-9

规格 价格 是否有货 数量
1 mg ¥2290 In-stock
5 mg ¥6860 In-stock
10 mg   询价  
50 mg   询价  

* Please select Quantity before adding items.

Procyanidin B3 相关产品

相关化合物库:

  • Natural Product Library Plus
  • Bioactive Compound Library Plus

生物活性

Procyanidin B3 is a natural product, acts as a specific HAT inhibitor, binds to the other site of p300 instead of the active site, selectively inhibits p300-mediated androgen receptor acetylation. Procyanidin B3 has no effect on HDAC or HMT (histone methyltransferase)[1].

IC50 & Target

p300[1]

分子量

578.52

Formula

C30H26O12

CAS 号

23567-23-9

中文名称

原花青素 B3;原花清素 B3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 50 mg/mL (86.43 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7285 mL 8.6427 mL 17.2855 mL
5 mM 0.3457 mL 1.7285 mL 3.4571 mL
10 mM 0.1729 mL 0.8643 mL 1.7285 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (4.32 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.32 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Choi KC, et al. Procyanidin B3, an inhibitor of histone acetyltransferase, enhances the action of antagonist for prostate cancer cells via inhibition of p300-dependent acetylation of androgen receptor. Biochem J. 2011 Jan 1;433(1):235-44.