Ac-DEVD-AMC; 纯度: 99.45%
Ac-DEVD-AMC 是 Caspase-3 的底物。
Ac-DEVD-AMC Chemical Structure
CAS No. : 169332-61-0
规格 | 价格 | 是否有货 | 数量 |
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1 mg | ¥500 | In-stock | |
5 mg | ¥1500 | In-stock | |
10 mg | ; | 询价 | ; |
50 mg | ; | 询价 | ; |
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Ac-DEVD-AMC 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
- Peptidomimetic Library
- Peptide Library
生物活性 |
Ac-DEVD-AMC is the Caspase-3 substrate. |
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体外研究 (In Vitro) |
Ac-DEVD-AMC is the caspase-3 substrate and could be used to monitor intracellular caspase-3 activity[1].The AMC moiety is highest at 2 h (area =5.6±1.8), the AMC peak area for Ac-DEVD-AMC incubated without lung specimen (substrate alone) for 6 h is relatively small[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
675.64 |
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Formula |
C30H37N5O13 |
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CAS 号 |
169332-61-0 |
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Sequence |
N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin |
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Sequence Shortening |
Ac-DEVD-7-amido-4-Methylcoumarin |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
Protect from light
*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light) |
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溶解性数据 |
In Vitro:;
H2O : 0.67 mg/mL (0.99 mM; Need ultrasonic) |
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参考文献 |
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Cell Assay [2] |
Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2 : 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through microcentrifuge filter, separateed on HPLC, and analyzed for the fluorogenic AMC moiety[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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