DiBAC4(3) | Cytiva思拓凡-Whatman滤纸滤膜滤器

DiBAC4(3); 纯度: ge;98.0%

DiBAC4(3) 是一种电压敏感的荧光染料 (λ_ex=490 nm，λ_em=505 nm)。

DiBAC4(3) Chemical Structure

CAS No. : 70363-83-6

规格	价格	是否有货	数量
10;mM;*;1 mL in DMSO	￥4880	In-stock
25 mg	￥3700	In-stock
50 mg	;	询价	;
100 mg	;	询价	;

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DiBAC4(3) 相关产品

bull;相关化合物库:

Bioactive Compound Library Plus

生物活性

DiBAC4(3) is a voltage-sensitive fluorescent dye (λ_ex=490 nm, λ_em=505 nm).

体外研究
(In Vitro)

The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

516.63

Formula

C₂₇H₄₀N₄O₆

CAS 号

70363-83-6

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据

In Vitro:;

DMSO : ≥ 60 mg/mL (116.14 mM)

* “≥” means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9356 mL	9.6781 mL	19.3562 mL
5 mM	0.3871 mL	1.9356 mL	3.8712 mL
10 mM	0.1936 mL	0.9678 mL	1.9356 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

参考文献

[1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50.

Cell Assay ^[1]	Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50.

Cell Assay
^[1]

Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50.