SV40 large T antigen NLS

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SV40 large T antigen NLS; 纯度: 99.80%

SV40 large T antigen NLS 是一种多肽，来源于 SV40 大 T 抗原 47-55 的氨基酸序列，能够将蛋白运输到细胞核。

SV40 large T antigen NLSamp;;

SV40 large T antigen NLS Chemical Structure

规格 价格 是否有货 数量
1 mg ￥800 In-stock
5 mg ￥3200 In-stock
10 mg ￥5600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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SV40 large T antigen NLS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

SV40 large T antigen NLS is from Large T antigen residue 47 to 55, enables protein import into cell nucleus.

体外研究
(In Vitro)

SV40 large tumor-antigen (T-ag) nuclear import is enhanced by the protein kinase CK2 (CK2) site (Ser111Ser112) flanking the nuclear localization sequence (NLS)[1]. T-ag NLS can enhance nuclear import, despite the fact that PK-C has been shown to inhibit nuclear import of proteins such as lamin B2[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1401.70

Formula

C58H104N20O18S
Sequence

Cys-Gly-Gly-Gly-Pro-Lys-Lys-Lys-Arg-Lys-Val-Glu-Asp
Sequence Shortening

CGGGPKKKRKVED
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Xiao CY, et al. Negative charge at the protein kinase CK2 site enhances recognition of the SV40 large T-antigen NLS by importin: effect of conformation. FEBS Lett. 1998 Dec 4;440(3):297-301.

    [2]. Xiao CY, et al. An engineered site for protein kinase C flanking the SV40 large T-antigen NLS confers phorbol ester-inducible nuclear import. FEBS Lett. 1998 Oct 9;436(3):313-7.