将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Hooper AM, et al. Isoschaftoside, a C-glycosylflavonoid from Desmodium uncinatum root exudate, is an allelochemical against the development of Striga. Phytochemistry. 2010 Jun;71(8-9):904-8.
[2]. Hamilton ML, et al. Elucidation of the biosynthesis of the di-C-glycosylflavone isoschaftoside, an allelopathic component from Desmodium spp. that inhibits Striga spp. development. Phytochemistry. 2012 Dec;84:169-76.
[3]. Shu Shan Du, et al. Nematocidal flavone-C-glycosides against the root-knot nematode (Meloidogyne incognita) from Arisaema erubescens tubers. Molecules. 2011 Jun 20;16(6):5079-86.
Osthenol (Ostenol), a prenylated coumarin isolated from the dried roots of Angelica pubescens, is selective, reversible, and competitive human monoamine oxidase-A (hMAO-A) inhibitor (Ki=0.26 µM). Osthenol potently inhibits recombinant hMAO-A with an IC50 of 0.74 µM and shows a high selectivity index for hMAO-A versus hMAO-B[1].
IC50 & Target[1]
hMAO-A
0.26 μM (Ki)
hMAO-A
0.74 μM (IC50)
分子量
230.26
Formula
C14H14O3
CAS 号
484-14-0
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Powder
-20°C
3 years
4°C
2 years
In solvent
-80°C
6 months
-20°C
1 month
参考文献
[1]. Baek SC, et al. Osthenol, a prenylated coumarin, as a monoamine oxidase A inhibitor with high selectivity. Bioorg Med Chem Lett. 2019;29(6):839-843.
[2]. Liao ZC, et al. Zhongguo Zhong Yao Za Zhi. 2017;42(15):2999-3003.
14-Deoxy-11-oxoandrographolide is an antileishmanial agent[1]. 14-Deoxy-11-oxoandrographolide inhibits the replication of heal chikungunya virus (CHIKV) and can be used for CHIKV infection research[1].
分子量
348.43
Formula
C20H28O5
CAS 号
42895-57-8
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献
[1]. S Lala, et al. Delivery in vivo of 14-deoxy-11-oxoandrographolide, an antileishmanial agent, by different drug carriers. Indian J Biochem Biophys. 2003 Jun;40(3):169-74.
[2]. G Koushik Kumar, et al. Structural basis for complementary and alternative medicine: Phytochemical interaction with non-structural protein 2 protease-a reverse engineering strategy. Chin J Integr Med. 2015 Jun;21(6):445-52.
LDS-751 是一种主要检测 DNA 的核酸染色剂。LDS-751 是一种主要检测DNA的核酸染色剂。LDS-751 对 DNA 有很高的亲和力,结合后荧光增强,但最大发射波长为 670nm。LDS-751 和 Thiazole orange 可用于红细胞、血小板、网织红细胞和有核细胞的分化,并能在 488nm 处激发。
LDS-751 Chemical Structure
CAS No. : 181885-68-7
规格
价格
是否有货
数量
5 mg
¥1950
In-stock
10 mg
;
询价
;
50 mg
;
询价
;
* Please select Quantity before adding items.
LDS-751 相关产品
bull;相关化合物库:
Bioactive Compound Library Plus
生物活性
LDS-751 is a nucleic acid stain principally detecting DNA. LDS-751 has high affinity for DNA and undergoes fluorescence enhancement upon binding, but with maximal emission at 670 nm. LDS-751 and Thiazole orange are used to differentiate erythrocytes, platelets, reticulocytes, and nucleated cells, and both can be excited at 488 nm[1][2][3].
体外研究 (In Vitro)
The LDS-751 permits the discrimination of intact cells from residual erythrocyte ghosts, platelets and damaged nucleated cells. The forward and orthogonal fight scattering signals of the intact cells, identified by LDS-751 staining allow a clear separation between the major leukocyte populations since the damaged nucleated cells, erythrocytes, erythrocyte cell ghosts and platelets are removed from the display[1].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量
471.98
Formula
C25H30ClN3O4
CAS 号
181885-68-7
运输条件
Room temperature in continental US; may vary elsewhere.
储存方式
-20deg;C, sealed storage, away from moisture and light
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献
[1]. Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12.
[2]. Terstappen LW, et al. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9(6):548-556.
[3]. McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163(2):155-160.
Cell Assay
Blood samples (1 mL) are obtained using a sterile Butterfly-21 needle and plastic syringe from the antecubital vein of normal healthy volunteers who have given their informed consent. They are immediately transferred to plastic tubes containing 17.3 mg of phenylmethylsulphonyl fluoride (PMSF) and 1 mL of LDS-751 at room temperature. Aliquots (25 μL) are incubated for 5 min with between 2 μL and 5 μL of undiluted monoclonal antibodies, then diluted with 0.5 mL of 1% BSA in Hepesbuffered Hanks’ balanced salts solution (HHBSS) and examined by flow cytometry[2].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献
[1]. Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12.
[2]. Terstappen LW, et al. Five-dimensional flow cytometry as a new approach for blood and bone marrow differentials. Cytometry. 1988;9(6):548-556.
[3]. McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993;163(2):155-160.