TMA-DPH | Cytiva思拓凡-Whatman滤纸滤膜滤器

TMA-DPH; 纯度: ge;98.0%

TMA-DPH 是一种疏水性荧光膜探针 (Ex=355 nm; Em=430 nm)。

TMA-DPH Chemical Structure

CAS No. : 115534-33-3

规格	价格	是否有货	数量
1 mg	￥1090	In-stock
5 mg	;	询价	;
10 mg	;	询价	;

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生物活性	TMA-DPH is a hydrophobic fluorescent membrane probe (Ex=355 nm; Em=430 nm).
体外研究 (In Vitro)	In contact with cells, TMA-DPH is instantaneously incorporated into the plasma membrane. TMA-DPH is concentrated in lysosomes and highly acidic late endosomes^[1]. The TMA-DPH fluorescence lifetime remains constant, 6.2±0.2 ns, below the same critical concentration of 2 μM, but decreases significantly with higher concentrations. This decrease, however, slows down above 5 μM. The TMA-DPH fluorescence anisotropy displays a similar evolution to the fluorescence lifetime. It is first constant, 0.283±0.003, again until 2 μM, and then decreases rapidly to 0.270±0.003 with 5 μM, continuing to fall, but at a lower rate with higher concentrations^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量	461.62
Formula	C₂₈H₃₁NO₃S
CAS 号	115534-33-3
运输条件	Room temperature in continental US; may vary elsewhere.
储存方式	-20deg;C, sealed storage, away from moisture and light *该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献	[1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94. [2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66.

Cell Assay ^[1]	L929 cells in 2 mL DM10F are allowed to adhere on microscope slide flasks. For the observation of plasma membrane labeling, cells are incubated for a short time (10 s) at room temperature with TMA-DPH 2×10^-6 M in PBS or in DM10F from a 4×10^-3 M stock solution in dimethylformamide. The unwashed slide is then transferred to the microscope and observed. For the labeling of internalized membrane, the cells are incubated in slide flasks at 37°C, with TMA-DPH 2×10^-6 M in DM10F for the desired time, and then washed by gently shaking the slide in PBS for a few seconds^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94. [2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66.

Cell Assay
^[1]

L929 cells in 2 mL DM10F are allowed to adhere on microscope slide flasks. For the observation of plasma membrane labeling, cells are incubated for a short time (10 s) at room temperature with TMA-DPH 2×10^-6 M in PBS or in DM10F from a 4×10^-3 M stock solution in dimethylformamide. The unwashed slide is then transferred to the microscope and observed. For the labeling of internalized membrane, the cells are incubated in slide flasks at 37°C, with TMA-DPH 2×10^-6 M in DM10F for the desired time, and then washed by gently shaking the slide in PBS for a few seconds^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Illinger D, et al. The kinetic aspects of intracellular fluorescence labeling with TMA-DPH support the maturation model for endocytosis in L929 cells. J Cell Biol. 1994 May;125(4):783-94.

[2]. Illinger D, et al. A comparison of the fluorescence properties of TMA-DPH as a probe for plasma membrane and for endocytic membrane. Biochim Biophys Acta. 1995 Oct 4;1239(1):58-66.