Gap 26

发表于由

Gap 26; 纯度: 99.64%

Gap 26 是一种连接蛋白 (connexin) 模拟肽，由连接蛋白 connexin 43 (gap junction 阻滞剂)第一个胞外环的第 63-75 残基组成，包含SHVR氨基酸基序。

Gap 26amp;;

Gap 26 Chemical Structure

CAS No. : 197250-15-0

规格 价格 是否有货 数量
1 mg ￥900 In-stock
5 mg ￥1800 In-stock
10 mg ￥2800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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Gap 26 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Gap 26 is a connexin mimetic peptide, composed of residue numbers 63-75 of the first extracellular loop of connexin 43 (gap junction blocker), containing the SHVR amino acid motif[1].

IC50 Target

Gap Junction Protein[1]
体外研究
(In Vitro)

Gap 26 (0.25 mg/mL, 30 min) reduces the wave size in the three cell lines (RBE4, SV-ARBEC and ECV304). Gap 26 (0.25 mg/mL, 30 min) completely abolishes the InsP3-triggered ATP response and reduced the ATP release to below the control level, indicating that the basal ATP release is also affected[1].
Gap 26 does indeed significantly inhibit our InsP3-triggered intercellular calcium waves, but it did not have any effect on dye coupling through junctional channels as evidenced by the FRAP experiments, despite the fact that connexin 43 was present in the cell lines used[1].
Gap 26 (100-300 μM) dose-dependently reduces the rhythmic responses of rabbit superior mesenteric arteries, with IC50 of 28.4 ± 3.4 μM[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1550.78

Formula

CHDFIKPS2V2Y
CAS 号

197250-15-0
Sequence

Val-Cys-Tyr-Asp-Lys-Ser-Phe-Pro-Ile-Ser-His-Val-Arg
Sequence Shortening

VCYDKSFPISHVR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

H2O : 50 mg/mL (32.24 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6448 mL 3.2242 mL 6.4484 mL
5 mM 0.1290 mL 0.6448 mL 1.2897 mL
10 mM 0.0645 mL 0.3224 mL 0.6448 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Katleen Braet, et al. Photoliberating inositol-1,4,5-trisphosphate triggers ATP release that is blocked by the connexin mimetic peptide gap 26. Cell Calcium. 2003 Jan;33(1):37-48.

    [2]. Chaytor AT, et al. Peptides homologous to extracellular loop motifs of connexin 43 reversibly abolish rhythmic contractile activity in rabbit arteries. J Physiol. 1997 Aug 15;503 ( Pt 1):99-110.