Crosstide

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Crosstide; 纯度: 95.70%

Crosstide 是一种糖原合成酶激酶α/β融合蛋白序列的模拟肽，是 Akt 的底物。

Crosstideamp;;

Crosstide Chemical Structure

CAS No. : 171783-05-4

规格 价格 是否有货 数量
1 mg ￥1100 In-stock
5 mg ￥4400 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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Crosstide 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptidomimetic Library
  • Peptide Library

生物活性

Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.

IC50 Target

Akt

;

体外研究
(In Vitro)

16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1164.23

Formula

C48H77N17O17
CAS 号

171783-05-4
Sequence

Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly
Sequence Shortening

GRPRTSSFAEG
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : ≥ 50 mg/mL (42.95 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8589 mL 4.2947 mL 8.5894 mL
5 mM 0.1718 mL 0.8589 mL 1.7179 mL
10 mM 0.0859 mL 0.4295 mL 0.8589 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Bentley JK, et al. Rhinovirus activates interleukin-8 expression via a Src/p110beta phosphatidylinositol 3-kinase/Akt pathway in human airway epithelial cells. J Virol. 2007 Feb;81(3):1186-94. Epub 2006 Nov 22.

    [2]. Baer K, et al. Activation of a GST-tagged AKT2/PKBbeta. Biochim Biophys Acta. 2005 Oct 10;1725(3):340-7. Epub 2005 Apr 20.
Kinase Assay
[1]

After serum deprivation for 24 h, cells are incubated with digoxigenin-labeled sham protein or digoxigenin-labeled RV39 at an MOI of 1.0 for 10 min. Cell homogenates are immunoprecipitated with mouse anti-digoxigenin antibody and precipitates incubated with Crosstide and [γ-32P]ATP. Crosstide is a glycogen synthase kinase α/β fusion protein sequence (GRPRTSSFAEG) which is a substrate for Akt. Samples are processed for autoradiography and immunoblotting using rabbit anti-phospho-Tyr416 Src, mouse anti-Src (clone GD11), rabbit anti-phospho-Ser473, or rabbit anti-Akt.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Bentley JK, et al. Rhinovirus activates interleukin-8 expression via a Src/p110beta phosphatidylinositol 3-kinase/Akt pathway in human airway epithelial cells. J Virol. 2007 Feb;81(3):1186-94. Epub 2006 Nov 22.

    [2]. Baer K, et al. Activation of a GST-tagged AKT2/PKBbeta. Biochim Biophys Acta. 2005 Oct 10;1725(3):340-7. Epub 2005 Apr 20.