Crosstide; 纯度: 95.70%
Crosstide 是一种糖原合成酶激酶α/β融合蛋白序列的模拟肽,是 Akt 的底物。
Crosstide Chemical Structure
CAS No. : 171783-05-4
规格 | 价格 | 是否有货 | 数量 |
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1 mg | ¥1100 | In-stock | |
5 mg | ¥4400 | In-stock | |
10 mg | ; | 询价 | ; |
50 mg | ; | 询价 | ; |
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Crosstide 相关产品
bull;相关化合物库:
- Bioactive Compound Library Plus
- Peptidomimetic Library
- Peptide Library
生物活性 |
Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt. |
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IC50 Target |
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体外研究 (In Vitro) |
16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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分子量 |
1164.23 |
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Formula |
C48H77N17O17 |
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CAS 号 |
171783-05-4 |
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Sequence |
Gly-Arg-Pro-Arg-Thr-Ser-Ser-Phe-Ala-Glu-Gly |
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Sequence Shortening |
GRPRTSSFAEG |
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运输条件 |
Room temperature in continental US; may vary elsewhere. |
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储存方式 |
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溶解性数据 |
In Vitro:;
H2O : ≥ 50 mg/mL (42.95 mM) * “≥” means soluble, but saturation unknown. 配制储备液
*
请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 |
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参考文献 |
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Kinase Assay [1] |
After serum deprivation for 24 h, cells are incubated with digoxigenin-labeled sham protein or digoxigenin-labeled RV39 at an MOI of 1.0 for 10 min. Cell homogenates are immunoprecipitated with mouse anti-digoxigenin antibody and precipitates incubated with Crosstide and [γ-32P]ATP. Crosstide is a glycogen synthase kinase α/β fusion protein sequence (GRPRTSSFAEG) which is a substrate for Akt. Samples are processed for autoradiography and immunoblotting using rabbit anti-phospho-Tyr416 Src, mouse anti-Src (clone GD11), rabbit anti-phospho-Ser473, or rabbit anti-Akt. MCE has not independently confirmed the accuracy of these methods. They are for reference only. |
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参考文献 |
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