Hoechst 33342 trihydrochloride(Synonyms: bisBenzimide H 33342 trihydrochloride HOE 33342 trihydrochloride)

发表于由

Hoechst 33342 trihydrochloride;(Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride) 纯度: 99.87%

Hoechst 33342 trihydrochloride是可以膜透细胞膜，发蓝色荧光的 DNA 染料。

Hoechst 33342 trihydrochlorideamp;;(Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride)

Hoechst 33342 trihydrochloride Chemical Structure

CAS No. : 875756-97-1

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Hoechst 33342 trihydrochloride 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.

IC50 Target

Dye reagent[1]
DNA Stain[1]
体外研究
(In Vitro)

Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

561.93

Formula

C27H31Cl3N6O
CAS 号

875756-97-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
溶解性数据
In Vitro:;

DMSO : ≥ 46 mg/mL (81.86 mM)

H2O : ≥ 5.6 mg/mL (9.97 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7796 mL 8.8979 mL 17.7958 mL
5 mM 0.3559 mL 1.7796 mL 3.5592 mL
10 mM 0.1780 mL 0.8898 mL 1.7796 mL

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.
Cell Assay
[1]

Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.