标签归档：hoechst

Hoechst 33258 analog

发表于由

Hoechst 33258 analog; 纯度: 99.96%

Hoechst 33258 analog 是DNA蓝色荧光染料。

Hoechst 33258 analogamp;;

Hoechst 33258 analog Chemical Structure

CAS No. : 258843-62-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥3932 In-stock
5 mg ￥3500 In-stock
10 mg ￥5000 In-stock
50 mg ￥15000 In-stock
100 mg ￥21000 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33258 analog 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33258 analog are part of a family of blue fluorescent dyes used to stain DNA. IC50 Value: Target: These Bis-benzimides were originally developed by Hoechst AG, which numbered all their compounds so that the dye Hoechst 33342 is the 33342nd compound made by the company. There are three related Hoechst stains: Hoechst 33258, Hoechst 33342, and Hoechst 34580. The dyes Hoechst 33258 and Hoechst 33342 are the ones most commonly used and they have similarexcitation/emission spectra. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescent light around anemission maximum at 461 nm. Unbound dye has its maximum fluorescence emission in the 510-540 nm range. Hoechst dyes are soluble in water and in organic solvents such as dimethyl formamide or dimethyl sulfoxide. Concentrations can be achieved of up to 10 mg/mL. Aqueous solutions are stable at 2-6 °C for at least six months when protected from light. For long-term storage the solutions are instead frozen at ≤-20 °C. The dyes bind to the minor groove of double-stranded DNA with a preference for sequences rich in adenine andthymine. Although the dyes can bind to all nucleic acids, AT-rich double-stranded DNA strands enhance fluorescence considerably. Hoechst dyes are cell-permeable and can bind to DNA in live or fixed cells. Therefore, these stains are often called supravital, which means that cells survive a treatment with these compounds. Cells that express specific ATP-binding cassette transporter proteins can also actively transport these stains out of their cytoplasm.

分子量

510.59

Formula

C29H30N6O3
CAS 号

258843-62-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : ≥ 51 mg/mL (99.88 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.9585 mL 9.7926 mL 19.5852 mL
5 mM 0.3917 mL 1.9585 mL 3.9170 mL
10 mM 0.1959 mL 0.9793 mL 1.9585 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. a b c “Hoechst Stains”. Invitrogren (Molecular Probes).

    [2]. Latt SA, Stetten G, Juergens LA, Recent developments in the detection of deoxyribonucleic acid synthesis by 33258 Hoechst fluorescence. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 23 (7): 493-505.

    [3]. Portugal J, Waring MJ. Assignment of DNA binding sites for 4′,6-diamidine-2-phenylindole and bisbenzimide (Hoechst 33258). A comparative footprinting study. Biochimica et Biophysica Acta 949 (2): 158-68.

Hoechst 33342 analog 2 trihydrochloride

发表于由

Hoechst 33342 analog 2 trihydrochloride; 纯度: 98.13%

Hoechst 33342 analog 2 trihydrochloride 是 Hoechst 33342 的一种类似物, Hoechst 33342 结合到 DNA 小沟，用于细胞 DNA 荧光染色。

Hoechst 33342 analog 2 trihydrochlorideamp;;

Hoechst 33342 analog 2 trihydrochloride Chemical Structure

CAS No. : 155815-98-8

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ￥5080 In-stock
5 mg ￥3500 In-stock
10 mg ￥5000 In-stock
50 mg ￥15000 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33342 analog 2 trihydrochloride 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33342 analog 2 trihydrochloride is a anglog of Hoechst 33342. Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

分子量

659.78

Formula

C25H26Cl3IN6O
CAS 号

155815-98-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

H2O : 20 mg/mL (30.31 mM; Need ultrasonic)

DMSO : 4 mg/mL (6.06 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.5157 mL 7.5783 mL 15.1566 mL
5 mM 0.3031 mL 1.5157 mL 3.0313 mL
10 mM 0.1516 mL 0.7578 mL 1.5157 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

Hoechst 33258 Solution Hoechst 33258 溶液(1 mg/ml 水溶液) 品牌：Chemodex

发表于由

Hoechst 33258 Solution

Hoechst 33258 溶液(1 mg/ml 水溶液)

品牌：Chemodex
CAS No.：23491-45-4
储存条件：+4°C
纯度：>98% (HPLC, TLC)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-B0446-L005

 – 5 ml – 1,680.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Hoechst 33342 Solution Hoechst 33258 溶液(1 mg/ml 水溶液) 品牌：Chemodex

发表于由

Hoechst 33342 Solution

Hoechst 33258 溶液(1 mg/ml 水溶液)

品牌：Chemodex
CAS No.：23491-52-3
储存条件：+4°C
纯度：>98% (HPLC)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-B0447-L005

 – 5 ml – 1,780.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Hoechst 34580 Solution Hoechst 33258 溶液(1 mg/ml 水溶液) 品牌：Chemodex

发表于由

Hoechst 34580 Solution

Hoechst 33258 溶液(1 mg/ml 水溶液)

品牌：Chemodex
CAS No.：911004-45-0
储存条件：+4°C
纯度：>98% (HPLC)
产品编号

(生产商编号)

 等级 规格 运输包装 零售价(RMB) 库存情况 参考值

CDX-B0448-L005

 – 5 ml – 3,370.00 – –


* 干冰运输、大包装及大批量的产品需酌情添加运输费用


* 零售价、促销产品折扣、运输费用、库存情况、产品及包装规格可能因各种原因有所变动，恕不另行通知，确切详情请联系上海金畔生物科技有限公司。

Hoechst 33342 trihydrochloride(Synonyms: bisBenzimide H 33342 trihydrochloride HOE 33342 trihydrochloride)

发表于由

Hoechst 33342 trihydrochloride;(Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride) 纯度: 99.87%

Hoechst 33342 trihydrochloride是可以膜透细胞膜，发蓝色荧光的 DNA 染料。

Hoechst 33342 trihydrochlorideamp;;(Synonyms: bisBenzimide H 33342 trihydrochloride; HOE 33342 trihydrochloride)

Hoechst 33342 trihydrochloride Chemical Structure

CAS No. : 875756-97-1

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
25 mg ￥500 In-stock
50 mg ￥800 In-stock
100 mg ￥1200 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33342 trihydrochloride 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33342 trihydrochloride is a membrane permeant blue fluorescent DNA stain.

IC50 Target

Dye reagent[1]
DNA Stain[1]
体外研究
(In Vitro)

Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

561.93

Formula

C27H31Cl3N6O
CAS 号

875756-97-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
溶解性数据
In Vitro:;

DMSO : ≥ 46 mg/mL (81.86 mM)

H2O : ≥ 5.6 mg/mL (9.97 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.7796 mL 8.8979 mL 17.7958 mL
5 mM 0.3559 mL 1.7796 mL 3.5592 mL
10 mM 0.1780 mL 0.8898 mL 1.7796 mL

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.
Cell Assay
[1]

Labeling Nuclear DNA with Hoechst 33342[1] Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+. Step 5, Mount the coverslips. Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

荧光染料Hoechst 33258(Synonyms: bisBenzimide H 33258 H 33258)

发表于由

荧光染料Dye Reagents Hoechst 33258;(Synonyms: bisBenzimide H 33258; H 33258)

Hoechst 33258是一种荧光染料，当与 dsDNA 结合时会发出蓝色荧光。

Hoechst 33258amp;;(Synonyms: bisBenzimide H 33258; H 33258)

Hoechst 33258 Chemical Structure

CAS No. : 23491-44-3

规格 价格 是否有货
10;mM;*;1 mL in DMSO ￥990 询问价格 货期
50 mg ￥900 询问价格 货期
100 mg ￥1300 询问价格 货期

* Please select Quantity before adding items.

Hoechst 33258 的其他形式现货产品:

Hoechst 33258 trihydrochloride Hoechst 33258 analog 5 Hoechst 33258 analog Hoechst 33258 analog 6 Hoechst 33258 analog 2 Hoechst 33258 analog 3

生物活性

Hoechst 33258 is a fluorescent dye that emits blue fluorescence when bound to dsDNA.

IC50 Target

IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42 ± 2.16 μM (U937 cell)[1]
体外研究
(In Vitro)

Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively[1]. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI50 from 84×10-6 to 191.5×10-6 mol/dm3. Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

424.50

Formula

C25H24N6O
CAS 号

23491-44-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 41.67 mg/mL (98.16 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3557 mL 11.7786 mL 23.5571 mL
5 mM 0.4711 mL 2.3557 mL 4.7114 mL
10 mM 0.2356 mL 1.1779 mL 2.3557 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.90 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.90 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

    [2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55.
Cell Assay
[2]

Hoechst 33258 is prepared as stock solutions in highly pure water. Working solutions in a concentration range of 10-3-10-6 mol/dm3 are prepared prior to testing. Cytotoxic effects of Hoechst 33258 on tested cell lines are determined by the MTT assay. Cells are seeded in 96 micro well flat bottom plates at a concentration of 2×104 cells/mL and left overnight in the CO2 incubator allowing them to attach to the plate surface. Growing medium is replaced with compound supplemented or control medium and incubated for 72 h. Fresh medium with 5 mg/mL of MTT is added onto cells and incubated for 4 h at 37°C. Upon media removal, water insoluble MTT-formazan crystals formed inside the living cells are dissolved in DMSO and the absorbance at 570 nm proportional to the number of living cells is measured on an Elisa Microplate Reader. All experiments are performed at least three times in triplicates.The GI50 value, defined as the compound concentration (μM) leading to cellular growth inhibition by 50%, is calculated and used as a parameter to compare cytotoxicity among the compounds[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

    [2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55.

荧光染料Hoechst 33258(Synonyms: bisBenzimide H 33258 H 33258)

发表于由

荧光染料Dye Reagents Hoechst 33258;(Synonyms: bisBenzimide H 33258; H 33258)

Hoechst 33258是一种荧光染料，当与 dsDNA 结合时会发出蓝色荧光。

Hoechst 33258(Synonyms: bisBenzimide H 33258; H 33258)

Hoechst 33258 Chemical Structure

CAS No. : 23491-44-3

规格 价格 是否有货
10;mM;*;1 mL in DMSO ￥990 询问价格 货期
50 mg ￥900 询问价格 货期
100 mg ￥1300 询问价格 货期

* Please select Quantity before adding items.

Hoechst 33258 的其他形式现货产品:

Hoechst 33258 trihydrochloride Hoechst 33258 analog 5 Hoechst 33258 analog Hoechst 33258 analog 6 Hoechst 33258 analog 2 Hoechst 33258 analog 3

生物活性

Hoechst 33258 is a fluorescent dye that emits blue fluorescence when bound to dsDNA.

IC50 Target

IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42 ± 2.16 μM (U937 cell)[1]
体外研究
(In Vitro)

Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively[1]. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI50 from 84×10-6 to 191.5×10-6 mol/dm3. Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

424.50

Formula

C25H24N6O
CAS 号

23491-44-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 41.67 mg/mL (98.16 mM; ultrasonic and warming and heat to 60°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.3557 mL 11.7786 mL 23.5571 mL
5 mM 0.4711 mL 2.3557 mL 4.7114 mL
10 mM 0.2356 mL 1.1779 mL 2.3557 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.90 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.90 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.90 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

    [2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55.
Cell Assay
[2]

Hoechst 33258 is prepared as stock solutions in highly pure water. Working solutions in a concentration range of 10-3-10-6 mol/dm3 are prepared prior to testing. Cytotoxic effects of Hoechst 33258 on tested cell lines are determined by the MTT assay. Cells are seeded in 96 micro well flat bottom plates at a concentration of 2×104 cells/mL and left overnight in the CO2 incubator allowing them to attach to the plate surface. Growing medium is replaced with compound supplemented or control medium and incubated for 72 h. Fresh medium with 5 mg/mL of MTT is added onto cells and incubated for 4 h at 37°C. Upon media removal, water insoluble MTT-formazan crystals formed inside the living cells are dissolved in DMSO and the absorbance at 570 nm proportional to the number of living cells is measured on an Elisa Microplate Reader. All experiments are performed at least three times in triplicates.The GI50 value, defined as the compound concentration (μM) leading to cellular growth inhibition by 50%, is calculated and used as a parameter to compare cytotoxicity among the compounds[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

    [2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55.

Hoechst 33258 trihydrochloride(Synonyms: bisBenzimide H 33258 trihydrochloride H 33258 trihydrochloride)

发表于由

Hoechst 33258 trihydrochloride;(Synonyms: bisBenzimide H 33258 trihydrochloride; H 33258 trihydrochloride) 纯度: 98.87%

Hoechst 33258 trihydrochloride (bisBenzimide H 33258 trihydrochloride) 是一种荧光染料，用于 DNA 染色。

Hoechst 33258 trihydrochlorideamp;;(Synonyms: bisBenzimide H 33258 trihydrochloride; H 33258 trihydrochloride)

Hoechst 33258 trihydrochloride Chemical Structure

CAS No. : 23491-45-4

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ￥660 In-stock
50 mg ￥600 In-stock
100 mg ￥1000 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33258 trihydrochloride 相关产品

bull;相关化合物库:

  • Anti-Blood Cancer Compound Library
  • Bioactive Compound Library Plus
  • Anti-Cancer Compound Library

生物活性

Hoechst 33258 trihydrochloride is a fluorescent dyes, which can be used as a cell dye for DNA.

IC50 Target

IC50: 51.31±4.56 μM (HeLa cell), 32.43±3.27 μM (HL60 cell), 15.42±2.16 μM (U937 cell)[1]
体外研究
(In Vitro)

Hoechst 33258, a fluorescent compound with a head-to-tail bis-benzimidazole structure, is initially found to be cytotoxic against L1210 murine leukemia. Hoechst 33258 is evaluated for their cytotoxicity against human tumor cell lines, which are cervix carcinoma cell line (HeLa), Human promyelocytic leukemia cell (HL60) and U937 cell Line. The IC50 determined in the case of HeLa, HL60 and U937 is 51.31±4.56, 32.43±3.27 and 15.42±2.16 μM for Hoechst 33258, respectively[1]. The cytotoxic property of Hoechst 33258 is investigated on a panel of seven tumour cell lines of different histological origin and Madine-Darby canine kidney (MDCK) normal cells. All cell lines, except MCF-7, exposed to Hoechst 33258 exhibit GI50 from 84×10-6 to 191.5×10-6 mol/dm3. Under the same experimental conditions, Hoechst 33258, used as a binder reference compound, stops the cell cycle in S phase and G0/G1[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

533.88

Formula

C25H27Cl3N6O
CAS 号

23491-45-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

H2O : ≥ 72.85 mg/mL (136.45 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8731 mL 9.3654 mL 18.7308 mL
5 mM 0.3746 mL 1.8731 mL 3.7462 mL
10 mM 0.1873 mL 0.9365 mL 1.8731 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

    [2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55.
Cell Assay
[2]

Hoechst 33258 is prepared as stock solutions in highly pure water. Working solutions in a concentration range of 10-3-10-6 mol/dm3 are prepared prior to testing. Cytotoxic effects of Hoechst 33258 on tested cell lines are determined by the MTT assay. Cells are seeded in 96 micro well flat bottom plates at a concentration of 2×104 cells/mL and left overnight in the CO2 incubator allowing them to attach to the plate surface. Growing medium is replaced with compound supplemented or control medium and incubated for 72 h. Fresh medium with 5 mg/mL of MTT is added onto cells and incubated for 4 h at 37°C. Upon media removal, water insoluble MTT-formazan crystals formed inside the living cells are dissolved in DMSO and the absorbance at 570 nm proportional to the number of living cells is measured on an Elisa Microplate Reader. All experiments are performed at least three times in triplicates.The GI50 value, defined as the compound concentration (μM) leading to cellular growth inhibition by 50%, is calculated and used as a parameter to compare cytotoxicity among the compounds[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Wang XJ, et al. Newly synthesized bis-benzimidazole derivatives exerting anti-tumor activity through inductionof apoptosis and autophagy. Bioorg Med Chem Lett. 2012 Oct 1;22(19):6297-300.

    [2]. Stolić I, et al. Synthesis, DNA/RNA affinity and antitumour activity of new aromatic diamidines linked by 3,4-ethylenedioxythiophene. Eur J Med Chem. 2011 Feb;46(2):743-55.

荧光染料Hoechst 33342 analog

发表于由

荧光染料Dye Reagents Hoechst 33342 analog; 纯度: 98.04%

Hoechst 33342 analog 是 Hoechst 33342 的一种类似物, Hoechst 33342 结合到 DNA 小沟，用于细胞 DNA 荧光染色。

Hoechst 33342 analogamp;;

Hoechst 33342 analog Chemical Structure

CAS No. : 178481-68-0

规格 价格 是否有货 数量
5 mg ￥5000 In-stock
10 mg ￥8500 In-stock
25 mg ￥18000 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Hoechst 33342 analog 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Hoechst 33342 analog is an anglog of Hoechst 33342, which is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.

分子量

590.59

Formula

C32H37Cl2N7
CAS 号

178481-68-0
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (169.32 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.6932 mL 8.4661 mL 16.9322 mL
5 mM 0.3386 mL 1.6932 mL 3.3864 mL
10 mM 0.1693 mL 0.8466 mL 1.6932 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (4.23 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (4.23 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.