[Glu1]-Fibrinopeptide B(Synonyms: [Glu1]-纤维蛋白肽B)

发表于由

[Glu1]-Fibrinopeptide B;(Synonyms: [Glu1]-纤维蛋白肽B)

[Glu1]-Fibrinopeptide B 衍生自纤维蛋白肽B氨基酸残基1-14。人纤维蛋白肽B（hFpB）是来自纤维蛋白原B β链的凝血酶衍生的蛋白水解裂解产物，可用于刺激中性粒细胞 (PMN)，单核细胞和成纤维细胞。

[Glu1]-Fibrinopeptide Bamp;;(Synonyms: [Glu1]-纤维蛋白肽B)

[Glu1]-Fibrinopeptide B Chemical Structure

CAS No. : 103213-49-6

规格 价格 是否有货
500 μg ￥1200 询问价格 货期
1 mg ￥1800 询问价格 货期
5 mg ￥7200 询问价格 货期

* Please select Quantity before adding items.

生物活性

[Glu1]-Fibrinopeptide B is derived from fibrinopeptide B amino acid residues 1-14. Human fibrinopeptide B (hFpB), a thrombin-derived proteolytic cleavage product of the fibrinogen B beta-chain, to stimulate neutrophils (PMN), monocytes, and fibroblasts.

体外研究
(In Vitro)

hFpB causes directed cell migration of PMN and fibroblasts that is optimal at approximately 10 nM. hFpB causes a rapid, dose-dependent increase in PMN cytoskeletal associated actin, but unlike fMLP, hFpB does not cause PMN aggregation, release of lysosomal enzymes (lysozyme and beta-glucuronidase), or the production of superoxide anion. These results suggest that hFpB may have a role in recruiting PMN and fibroblasts at sites of fibrin deposition and turnover. The capacity of hFpB to cause PMN chemotaxis without causing concurrent release of lysosomal enzymes or the production of superoxide anion is further evidence for the complexity of PMN responses to chemotactic agents[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1570.60

Formula

C66H95N19O26
CAS 号

103213-49-6
Sequence

Glu-Gly-Val-Asn-Asp-Asn-Glu-Glu-Gly-Phe-Phe-Ser-Ala-Arg
Sequence Shortening

EGVNDNEEGFFSAR
中文名称

[Glu1]-纤维蛋白肽B
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Senior RM, et al. Effects of fibrinogen derivatives upon the inflammatory response. Studies with human fibrinopeptide B. J Clin Invest. 1986 Mar;77(3):1014-9.