Apamin(Synonyms: Apamine)

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Apamin;(Synonyms: Apamine)

Apamin (Apamine) 是在蜂毒素中发现的一种 18 个氨基酸的肽类神经毒素，是一种 Ca2+ 激活的 K+ (SK) 通道的特异性和选择性的阻断剂，具有抗炎和抗纤维化的作用。

Apaminamp;;(Synonyms: Apamine)

Apamin Chemical Structure

CAS No. : 24345-16-2

规格 价格 是否有货
500 μg ￥1500 询问价格 货期
1 mg ￥2500 询问价格 货期

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Apamin 的其他形式现货产品:

Apamin TFA

生物活性

Apamin (Apamine) is an 18 amino acid peptide neurotoxin found in apitoxin (bee venom), is known as a specifically selective blocker of Ca2+-activated K+ (SK) channels and exhibits anti-inflammatory and anti-fibrotic activity[1].

IC50 Target

K+ channel[1]
体外研究
(In Vitro)

Apamin (0.5-2 µg/mL; 24 hours; HSC-T6 cells) treatment markedly reduces the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Apamin treatment abrogats the activation of p-Smad2/3 and Smad4 induced by TGF-β1[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: HSC-T6 cells
Concentration: 0.5 µg/mL, 1 µg/mL and 2 µg/mL
Incubation Time: 24 hours
Result: Markedly reduced the expression of α-SMA in the TGF-β1-induced HSC-T6 cells. Abrogated the activation of p-Smad2/3 and Smad4 induced by TGF-β1.

体内研究
(In Vivo)

Apamin (0.1 mg/kg; intraperitoneal injection; twice a week; for 4 weeks; C57BL/6 male mice) treatment results in decreased liver injury and proinflammatory cytokine levels. Apamin suppresses the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC)-fed mice[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 8-week-old C57BL/6 male mice (20-25 g) with DDC feeding[1]
Dosage: 0.1 mg/kg
Administration: Intraperitoneal injection; twice a week; for 4 weeks
Result: Resulted in decreased liver injury and proinflammatory cytokine levels. Suppressed the deposition of collagen, proliferation of BECs and expression of fibrogenic genes in the DDC-fed mice.

分子量

2027.34

Formula

C79H131N31O24S4
CAS 号

24345-16-2
Sequence

Cys-Asn-Cys-Lys-Ala-Pro-Glu-Thr-Ala-Leu-Cys-Ala-Arg-Arg-Cys-Gln-Gln-His-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)
Sequence Shortening

CNCKAPETALCARRCQQH-NH2 (Disulfide bridge: Cys1-Cys11;Cys3-Cys15)
中文名称

蜂毒明肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kim JY, et al. Apamin suppresses biliary fibrosis and activation of hepatic stellate cells. Int J Mol Med. 2017 May;39(5):1188-1194.