NS2 (114-121), Influenza

发表于由

NS2 (114-121), Influenza;

NS2 (114-121), Influenza，是流感非结构蛋白 2 (NS2) 的 114-121 片段，是流感衍生的抗原决定簇。NS2 (114-121), Influenza 可用于研究 CD8+ 细胞毒性 T 淋巴细胞 (CTL) 的抗病毒免疫反应。

NS2 (114-121), Influenzaamp;;

NS2 (114-121), Influenza Chemical Structure

CAS No. : 184763-32-4

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生物活性

NS2 (114-121), Influenza, the 114-121 fragment of influenza nonstructural protein 2 (NS2), is a influenza-derived epitope. NS2 (114-121), Influenza can be used for the research of CD8+ cytotoxic T lymphocyte (CTL) in antiviral immune responses[1].

分子量

1011.17

Formula

C48H74N12O12
CAS 号

184763-32-4
Sequence

Arg-Thr-Phe-Ser-Phe-Gln-Leu-Ile
Sequence Shortening

RTFSFQLI
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Paul G Thomas, et al. Ecological analysis of antigen-specific CTL repertoires defines the relationship between naive and immune T-cell populations. Proc Natl Acad Sci U S A. 2013 Jan 29;110(5):1839-44.