荧光染料6-TAMRA-SE(Synonyms: 6-TAMRA-NHS ester 6-Carboxytetramethylrhodamine N-succinimidyl ester)

荧光染料Dye Reagents 6-TAMRA-SE;(Synonyms: 6-TAMRA-NHS ester; 6-Carboxytetramethylrhodamine N-succinimidyl ester) 纯度: ge;95.0%

6-TAMRA-SE (6-TAMRA-NHS ester) 是一种带有胺反应基的荧光染料。6-TAMRA-SE 是用于 DNA 自动测序的传统荧光团之一。

6-TAMRA-SEamp;;(Synonyms: 6-TAMRA-NHS ester;  6-Carboxytetramethylrhodamine N-succinimidyl ester)

6-TAMRA-SE Chemical Structure

CAS No. : 150810-69-8

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥1625 In-stock
1 mg ¥600 In-stock
5 mg ¥1400 In-stock
10 mg ¥2000 In-stock
50 mg ¥7500 In-stock
100 mg ; 询价 ;
200 mg ; 询价 ;

* Please select Quantity before adding items.

6-TAMRA-SE 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

6-TAMRA-SE (6-TAMRA-NHS ester) is a fluorescent dye carrying the amine reactive group. 6-TAMRA-SE is one of the traditional fluorophores used for automated DNA sequencing[1][2][3].

体外研究
(In Vitro)

6-TAMRA-SE (6-TAMRA-NHS ester) is an amine-reactive form of tetramethylrhodamine. 6-TAMRA-SE (soluble in DMF and DMSO, absorption=546, emission=576 measured in MeOH ϵ=9.5×104ϵ=9.5×104)[1][3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

527.52

Formula

C29H25N3O7

CAS 号

150810-69-8

中文名称

6-羧基四甲基罗丹明琥珀酰亚胺酯

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)

溶解性数据
In Vitro:;

DMSO : 100 mg/mL (189.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8957 mL 9.4783 mL 18.9566 mL
5 mM 0.3791 mL 1.8957 mL 3.7913 mL
10 mM 0.1896 mL 0.9478 mL 1.8957 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.5 mg/mL (4.74 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (4.74 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Wang Y, et al. RNA molecules that specifically and stoichiometrically bind aminoglycoside antibiotics with high affinities. Biochemistry. 1996 Sep 24;35(38):12338-46.

    [2]. Tessmar J, et al. Toward the development of biomimetic polymers by protein immobilization: PEGylation of insulin as a model reaction. Tissue Eng. 2004;10(3-4):441-453.

    [3]. Thomas TP, et al. Investigation of tumor cell targeting of a dendrimer nanoparticle using a double-clad optical fiber probe. J Biomed Opt. 2008;13(1):014024.