3X FLAG peptide TFA

3X FLAG peptide TFA; 纯度: 99.79%

3X FLAG peptide TFA 是一种合成的多肽,包含三个重复的 Asp-Tyr-Lys-Xaa-Xaa-Asp 基序。

3X FLAG peptide TFAamp;;

3X FLAG peptide TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ¥600 In-stock
5 mg ¥1200 In-stock
10 mg ¥2100 In-stock
25 mg ¥4200 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

3X FLAG peptide TFA is a synthetic peptide with a 3-time repeated DYKXXD motif[1].

体外研究
(In Vitro)

3X FLAG peptide can be used to label protein and other molecules.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

2975.84

Formula

C122H170F3N31O51S

Sequence

Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

Sequence Shortening

MDYKDHDGDYKDHDIDYKDDDDK

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 25 mg/mL (8.40 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.3360 mL 1.6802 mL 3.3604 mL
5 mM 0.0672 mL 0.3360 mL 0.6721 mL
10 mM

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;PBS

    Solubility: 50 mg/mL (16.80 mM); Clear solution; Need ultrasonic and adjust pH to 3 with HCl

  • 2.

    请依序添加每种溶剂:;20 mM Tris pH7.5, 100 mM NaCl

    Solubility: 33.33 mg/mL (11.20 mM); Clear solution; Need ultrasonic

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Zilio N, et al. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2017 Mar 1;2017(3):pdb.prot091611.

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