Jaceosidin(Synonyms: 棕矢车菊素)

发表于由

天然产物 黄酮类 Flavonoids

Jaceosidin (Synonyms: 棕矢车菊素) 纯度: 98.00%

Jaceosidin 是从 Artemisia vestita 中得到的黄酮类化合物，可激活 Bax，下调 Mcl-1 和 c-FLIP 的表达，诱导癌细胞凋亡。Jaceosidin 具有抗癌和抗炎作用，能够降低炎性因子水平，激活 NF-κB，抑制 COX-2 的表达。

Jaceosidin(Synonyms: 棕矢车菊素)

Jaceosidin Chemical Structure

CAS No. : 18085-97-7

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ￥1017 In-stock
1 mg ￥600 In-stock
5 mg ￥1400 In-stock
10 mg ￥2200 In-stock
25 mg ￥4500 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Jaceosidin 相关产品

•相关化合物库:

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生物活性

Jaceosidin is a flavonoid isolated from Artemisia vestita, induces apoptosis in cancer cells, activates Bax and down-regulates Mcl-1 and c-FLIP expression[1]. Jaceosidin exhibits anti-cancer[2], anti-inflammatory activities, decreases leves of inflammatory markers, and suppresses COX-2 expression and NF-κB activation[3].

IC50 & Target[1][3]

Bax

 

COX-2

 

体外研究
(In Vitro)

Jaceosidin (30, 50, 75 μM) induces apoptosis in human renal carcinoma Caki cells after treatment for 24 h, shows no obvious effect on normal cells[1].
Jaceosidin (75 μM) reduces MMP levels and causes cytochrome c release into the cytoplasm through Bax activation[1].
Jaceosidin-mediated apoptosis is involved in downregulation of Mcl-1, c-FLIP expression, which is via inhibition of NF-κB and/or Sp1 transcriptional activity[1].
Jaceosidin shows cytostatic activity to HES and HESC cells with IC50s of 52.68 and 55.10 μM, and is less cytotocxic on Hec1 A and KLE (IC50, 70.54, 147.14 μM, respectively), after treatment for 48 h[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[2]

Cell Line: Hec1A, KLE, HES and HESC cells
Concentration: 3.125, 6.25, 12.5, 25, 50, and 100 μM
Incubation Time: 48 hour
Result: Showed cytostatic activity to HES and HESC cells with IC50s of 52.68 and 55.10 μM, less cytotocxic on Hec1 A and KLE (IC50, 70.54, 147.14 μM).

体内研究
(In Vivo)

Jaceosidin (10 and 20 mg/kg, p.o., once a day for 3 days) blocks carrageenan-induced increase in leukocyte number and protein levels in air pouch exudates in mice[3].
Jaceosidin (10, 20 mg/kg, p.o.) suppresses COX-2 expression and NF-κB activation in mice[3].
Jaceosidin (20 mg/kg, p.o. for 2 hours) reduces hind paw edema volume in rats[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 5-week-old male BALB/c mice (23-26 g)[3]
Dosage: 10 and 20 mg/kg
Administration: P.O. once a day for 3 days
Result: Decreased the volumes of exudates (inflammatory markers), cell number and protein levels. Inhibited TNF-α by 46.7% and 50.8%, IL-1β by 46.0% and 44.7%, and PGE2 by 21.7% and 16.9%, respectively, at 20 mg/kg. Blocked COX-2 expression and NF-κB activation.
Animal Model: Male Sprague-Dawley rats (180-200 g)[3]
Dosage: 20 mg/kg
Administration: P.O., for 2 hour
Result: Reduced hind paw edema volume by 27.1% at 1 h, and 24.0% at 2 h, respectively.

分子量

330.29

Formula

C17H14O7
CAS 号

18085-97-7
中文名称

棕矢车菊素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : 125 mg/mL (378.46 mM; Need ultrasonic)

Ethanol : 7.14 mg/mL (21.62 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.0276 mL 15.1382 mL 30.2764 mL
5 mM 0.6055 mL 3.0276 mL 6.0553 mL
10 mM 0.3028 mL 1.5138 mL 3.0276 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.30 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.30 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂： 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.30 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.30 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂： 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.30 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.30 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献

  • [1]. Woo SM, et al. Jaceosidin induces apoptosis through Bax activation and down-regulation of Mcl-1 and c-FLIP expression in human renal carcinoma Caki cells. Chem Biol Interact. 2016 Dec 25;260:168-175.

    [2]. Lee JG, et al. Jaceosidin, isolated from dietary mugwort (Artemisia princeps), induces G2/M cell cycle arrest by inactivating cdc25C-cdc2 via ATM-Chk1/2 activation. Food Chem Toxicol. 2013 May;55:214-21.

    [3]. Min SW, et al. Inhibitory effect of eupatilin and jaceosidin isolated from Artemisia princeps on carrageenan-induced inflammation in mice. J Ethnopharmacol. 2009 Sep 25;125(3):497-500.