Safflower yellow is extracted from the flowers of the plant safflower (Carthamus tinctorius) and as the traditional Chinese medicine it has been extensively used for the treatment of cardio cerebrovascular diseases.

Safflower yellow (SY) is the safflower extract and is the one of traditional Chinese medicine. Safflower yellow can promote blood circulation, remove blood stasis, and thereby improve capillary circulation at the site of tissue injury. Safflower yellow is mixtures of a water-soluble chalcone component, in which both hydroxyl safflower yellow A (HSYA) and safflower yellow B (SYB) are the main components. Safflower injection excellently protects the heart by way of improving functions of cardiac contraction and dilation, increasing coronary blood flow, and strengthening the bcl-2 (anti apoptosis gene) protein expression. Safflower yellow alleviates the injured tendon adhesion and inflammatory reaction and promoted the repair of injured tendon^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

1401-20-3

红花黄

Room temperature in continental US; may vary elsewhere.

DMSO : 20 mg/mL (Need ultrasonic)

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

  此方案可获得 ≥ 2 mg/mL (Infinity mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2 mg/mL (Infinity mM); Clear solution

  此方案可获得 ≥ 2 mg/mL (Infinity mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

• [1]. Zhou D, et al. The effect of safflower yellow on spinal cord ischemia reperfusion injury in rabbits. Oxid Med Cell Longev. 2013;2013:692302.

Rabbits^[1]
The adult male New Zealand rabbits (body weight 2.0-2.5 kg) are used. Twenty-four rabbits are randomly divided into three groups (per group): sham-operated control (Cont), spinal cord ischemia reperfusion, and treated with safflower yellow. The control group only execute anesthesia and surgical procedures, except for occluding the abdominal aorta. The group is intravenously injected with 2 mL/kg of a solution of 16% (wt/vol) Safflower yellow (1 mL, containing 1.6 mg Safflower yellow), followed by continuous infusion of a total of 5 mL/kg through the right femoral vein at the moment of reperfusion beginning after 40 minutes of the abdominal aorta occlusion. The same volumes of 0.9% saline solution are administrated in control and groups. Blood samples are obtained at the end of 0 hour, 4 hours, 12 hours, 24 hours, and 48 hours after reperfusion, and the plasma is separated and stored at -80°C for further analysis. All animals are sacrificed 48 hours after reperfusion and are rapidly perfused with 0.9% sodium chloride, and the L2-5 segments of the spinal cord are quickly removed. The L2-3 segment in each animal is used for western blot, and the other segment (L4-5) is immersed into 10% neutral formaldehyde for 2-3 days and is used for morphology analysis^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Zhou D, et al. The effect of safflower yellow on spinal cord ischemia reperfusion injury in rabbits. Oxid Med Cell Longev. 2013;2013:692302.