7-Aminoactinomycin D(Synonyms: 7-AAD)

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7-Aminoactinomycin D;(Synonyms: 7-AAD) 纯度: ge;99.0%

7-Aminoactinomycin D (7-AAD) 是一种荧光 DNA 染料，是一种有效的 RNA 聚合酶 (RNA polymerase) 抑制剂。7-Aminoactinomycin D 选择性结合到 DNA 的 GC 区域，还具有抗菌作用。

7-Aminoactinomycin Damp;;(Synonyms: 7-AAD)

7-Aminoactinomycin D Chemical Structure

CAS No. : 7240-37-1

规格 价格 是否有货 数量
1 mg ￥2300 In-stock
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7-Aminoactinomycin D 相关产品

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  • Bioactive Compound Library Plus

生物活性

7-Aminoactinomycin D (7-AAD) a fluorescent DNA stain, is a potent RNA polymerase inhibitor. 7-Aminoactinomycin D selectively binds to GC regions of the DNA. 7-Aminoactinomycin D also has antibacterial effects[1][2][3].

体外研究
(In Vitro)

7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. 7-Aminoactinomycin D staining with 5 μg/mL, 10 μg/mL, and 20 μg/mL, but not with 1 μg/mL, is suitable for quantification of apoptosis in flow cytometry[1].
7-Aminoactinomycin D is frequently used to stain and exclude dead cells in flow cytometry at low concentrations (0.5-5 μg/mL). At higher concentrations (10-20 μg/mL), 7-Aminoactinomycin D has also been used to distinguish between viable cells (7-AADnegative) and apoptotic cells (7-AADdim) or dead cells (7-AADbright) using the fact that permeability of the cell membrane, and hence fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1270.43

Formula

C62H87N13O16
CAS 号

7240-37-1
中文名称

7-氨基放线菌素D
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (78.71 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7871 mL 3.9357 mL 7.8714 mL
5 mM 0.1574 mL 0.7871 mL 1.5743 mL
10 mM 0.0787 mL 0.3936 mL 0.7871 mL

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Wadkins RM, et al. Actinomycin D and 7-aminoactinomycin D binding to single-stranded DNA. Biochemistry. 1991 Oct 1;30(39):9469-78.

    [2]. Nadine C L Zembruski, et al. 7-Aminoactinomycin D for apoptosis staining in flow cytometry. Anal Biochem. 2012 Oct 1;429(1):79-81.

    [3]. Hanen Sellami, et al. Molecular detection of Chlamydia trachomatis and other sexually transmitted bacteria in semen of male partners of infertile couples in Tunisia: the effect on semen parameters and spermatozoa apoptosis markers. PLoS One. 2014 Jul 14;9(7):e98903.