Deoxycytidine triphosphate trisodium salt(Synonyms: dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt)

生化分析试剂 Biochemical Assay Reagents
Deoxycytidine triphosphate trisodium salt;(Synonyms: dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt) 纯度: ge;97.0%

Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) 是可用于 DNA 合成 (DNA synthesis) 的核苷三磷酸。Deoxycytidine triphosphate trisodium salt 可用于实时 PCR,cDNA 合成 和 DNA 测序等研究。

Deoxycytidine triphosphate trisodium salt(Synonyms: dCTP trisodium salt; 2′-Deoxycytidine-5′-triphosphate trisodium salt)

Deoxycytidine triphosphate trisodium salt Chemical Structure

CAS No. : 109909-44-6

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50 mg ¥1800 In-stock
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生物活性

Deoxycytidine triphosphate trisodium salt (dCTP trisodium salt) is a nucleoside triphosphate that can be used for DNA synthesis. Deoxycytidine triphosphate trisodium salt has many applications, such as real-time PCR, cDNA synthesis, and DNA sequencing[1][2][3].

体外研究
(In Vitro)

Assays of thirteen cell lines, derived from mouse lymphomas, myelomas, myeloid tumors, and a mastocytoma, for sensitivity to growth inhibition by 1-β-D-arabinofuranosylcytosine (ara-C) revealed a spectrum between the most and least sensitive which differed 100-fold from each other. An inverse correlation between sensitivity and cellular Deoxycytidine triphosphate (2′-Deoxycytidine-5′-triphosphate, dCTP) content is found, and this suggested that sensitivity of cells might be increased if the dCTP content was lowered during cell exposure to ara-C[1].
Deoxycytidine triphosphate trisodium salt is a phosphoethanolamine cytidylyltransferase substrate[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

533.10

Formula

C9H13N3Na3O13P3

CAS 号

109909-44-6

中文名称

脱氧胞苷三磷酸三钠盐;2′-脱氧胞苷-5′-三磷酸三钠盐

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 250 mg/mL (468.96 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8758 mL 9.3791 mL 18.7582 mL
5 mM 0.3752 mL 1.8758 mL 3.7516 mL
10 mM 0.1876 mL 0.9379 mL 1.8758 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. A W Harris, et al. Effect of thymidine on the sensitivity of cultured mouse tumor cells to 1-beta-D-arabinofuranosylcytosine. Cancer Res. 1979 Feb;39(2 Pt 1):538-41.

    [2]. B A Bladergroen, et al. CTP:phosphoethanolamine cytidylyltransferase. Biochim Biophys Acta. 1997 Sep 4;1348(1-2):91-9.

    [3]. M H Tattersall, et al. Deoxyribonucleoside triphosphates in human cells: changes in disease and following exposure to drugs. Eur J Clin Invest. 1975 Apr;5(2):191-202.