Neobavaisoflavone(Synonyms: 新补骨脂异黄酮)

天然产物 黄酮类 Flavonoids

Neobavaisoflavone (Synonyms: 新补骨脂异黄酮) 纯度: 99.91%

Neobavaisoflavone,是一种从 Psoralea corylifolia 的种子中分离出来的类黄酮。Neobavaisoflavone 具有抗炎,抗癌和抗氧化的作用。Neobavaisoflavone 在中至高浓度下可抑制 DNA 聚合酶 (DNA polymerase)。Neobavaisoflavone 也可抑制血小板聚集。

Neobavaisoflavone(Synonyms: 新补骨脂异黄酮)

Neobavaisoflavone Chemical Structure

CAS No. : 41060-15-5

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥605 In-stock
5 mg ¥550 In-stock
10 mg ¥820 In-stock
50 mg   询价  
100 mg   询价  

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Neobavaisoflavone 相关产品

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生物活性

Neobavaisoflavone, a flavonoid, is isolated from the seeds of Psoralea corylifolia. Neobavaisoflavone exhibits anti-inflammatory, anti-cancer and anti-oxidation activities. Neobavaisoflavone inhibits DNA polymerase at moderate to high concentrations. Neobavaisoflavone also inhibits platelet aggregation[1][2][3][4][5].

IC50 & Target

DNA polymerase[4]

体外研究
(In Vitro)

Neobavaisoflavone (1-50 μM; 20 h) decreases NO (ED50=25 μM) and cytokine (ED50s=23.11, 5.03, 5.23, 5.26 and 18.80 μM for IL-1β, IL-6, IL-12p40, IL-12p70 and TNF-α, respectively) production in LPS plus IFN-γ-stimulated RAW264.7 macrophages[1].
Neobavaisoflavone (1-100 μM; 30 min) decreases the chemiluminescence in PMA-stimulated RAW264.7 macrophages, with an ED50 of 19.94 μM in activated RAW264.7 cells[1].
Neobavaisoflavone (1-100 μM); 20 h) has no effect on the viability and is not toxic to RAW264.7 cells[1].
Neobavaisoflavone (20-50 μM; 48 h) inhibits prostate cancer cell proliferation by inducing cytotoxicity and apoptosis in a dose-dependent manner[2].
Neobavaisoflavone (2-8 μM; 7 d) inhibits RANKL‐mediated osteoclastogenesis in bone marrow monocytes (BMMCs) and RAW264.7 cells dose dependently at the early stage[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Neobavaisoflavone (30 mg/kg; i.p. for 6 weeks) inhibits osteoclastogenesis, promotes osteogenesis and ameliorates bone loss in ovariectomized mice[3].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: C57BL/6 female mice (8 weeksd; 20-25 g) were removed bilateral ovaries[3]
Dosage: 30 mg/kg
Administration: I.p. for 6 weeks
Result: Attenuated bone loss by inhibiting osteoclast activation and promoting osteogenesis in ovariectomized mice.

分子量

322.35

Formula

C20H18O4

CAS 号

41060-15-5

中文名称

新补骨脂异黄酮

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
溶解性数据
In Vitro: 

DMSO : ≥ 31 mg/mL (96.17 mM)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.1022 mL 15.5111 mL 31.0222 mL
5 mM 0.6204 mL 3.1022 mL 6.2044 mL
10 mM 0.3102 mL 1.5511 mL 3.1022 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (6.45 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.45 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (6.45 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.45 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂: 10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (6.45 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (6.45 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Szliszka E, et, al. Inhibition of inflammatory mediators by neobavaisoflavone in activated RAW264.7 macrophages. Molecules. 2011 May 3;16(5):3701-12.

    [2]. Szliszka E, et, al. Enhanced TRAIL-mediated apoptosis in prostate cancer cells by the bioactive compounds neobavaisoflavone and psoralidin isolated from Psoralea corylifolia. Pharmacol Rep. 2011;63(1):139-48.

    [3]. Chen H, et, al. Neobavaisoflavone inhibits osteoclastogenesis through blocking RANKL signalling-mediated TRAF6 and c-Src recruitment and NF-κB, MAPK and Akt pathways. J Cell Mol Med. 2020 Aug;24(16):9067-9084.

    [4]. Sun NJ, et, al. DNA polymerase and topoisomerase II inhibitors from Psoralea corylifolia. J Nat Prod. 1998 Mar;61(3):362-6.

    [5]. Tsai WJ, et, al. Antiplatelet flavonoids from seeds of Psoralea corylifolia. J Nat Prod. 1996 Jul;59(7):671-2.

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