OVA Peptide(257-264) TFA

OVA Peptide(257-264) TFA; 纯度: 98.78%

OVA Peptide(257-264) TFA 是 OVA 的 I 类 (Kb)-限制性肽表位,OVA 是由 I 类 MHC 分子 H-2Kb 呈递的来自卵清蛋白的八聚体肽。

OVA Peptide(257-264) TFAamp;;

OVA Peptide(257-264) TFA Chemical Structure

CAS No. : 1262751-08-5

规格 价格 是否有货 数量
1 mg ¥900 In-stock
5 mg ¥3600 In-stock
10 mg ¥5600 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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OVA Peptide(257-264) TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

OVA Peptide(257-264) TFA is a class I (Kb)-restricted peptide epitope of OVA, an octameric peptide from ovalbumin presented by the class I MHC molecule, H-2Kb.

体外研究
(In Vitro)

TAP1-I- and C57BL/6 macrophages may process Crl-OVA and full-length OVA in different cellular compartments and that the protein context of the OVA Peptide(257-264) epitope influences the extent of TAP-independent processing for MHC class I presentation. OVA Peptide(257-264) epitope is presented with a differential dependence on the TAP transporter depending on the protein context of the OVA epitope: OVA Peptide(257-264) contained within the MBPCrl-OVA or Crl-OVA bacterial fusion proteins is presented with little dependence on the TAP transporter, while OVA Peptide(257-264) contained within full-length ovalbumin is largely dependent on the TAP transporter, regardless of whether recombinant OVA is expressed in bacteria or the native protein is coupled to polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

1077.15

Formula

C47H75F3N10O15

CAS 号

1262751-08-5

Sequence

Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu

Sequence Shortening

SIINFEKL

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

溶解性数据
In Vitro:;

H2O : 20 mg/mL (18.57 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9284 mL 4.6419 mL 9.2838 mL
5 mM 0.1857 mL 0.9284 mL 1.8568 mL
10 mM 0.0928 mL 0.4642 mL 0.9284 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (1.93 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (1.93 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9.

Cell Assay

TAPl-/- or C57BL/6 macrophages are co-incubated with either bacteria or polystyrene beads containing the 257-264 epitope from ovalbumin [OVA Peptide(257-264)], which binds the mouse class I molecule Kb. The source of the OVA(257-264) epitope is either the Crl-OVA(257-264) (Crl-OVA) fusion protein, the maltose binding protein (MBP)-Crl-OVA fusion protein, native OVA or bacterial recombinant OVA (rOVA); Crl-OVA, MBP-Crl-OVA and rOVA are each expressed in bacteria, and Crl-OVA and MBP-Crl-OVA purified from bacterial lysates and native egg OVA are coated onto polystyrene beads[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Wick MJ, et al. Major histocompatibility complex class I presentation of ovalbumin peptide 257-264 from exogenous sources: protein context influences the degree of TAP-independent presentation. Eur J Immunol. 1996 Nov;26(11):2790-9.