Sulforhodamine 101 (SR101) is an amphoteric rhodamine red fluorescent dye (excitation/emission: 586/605 nm). Sulforhodamine 101 is used extensively for investigating neuronal morphology and acts as a cell type-selective fluorescent marker of astrocytes both in vivo and in slice preparations^[1].

Sulforhodamine 101 (SR101) does not label astrocytes in brainstem slices as strong and specific as in the hippocampus or cortex. To minimize excitatory side effects, the concentration of Sulforhodamine 101 has to be kept as low as possible or the labeling procedure can be performed after the actual experiment^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In vivo, epileptic activity can be induced by intra-hippocampal injection of small volumes of 10 μM Sulforhodamine 101 or topical application of 100 μM^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

606.71

C₃₁H₃₀N₂O₇S₂

60311-02-6

磺基罗丹明101

Room temperature in continental US; may vary elsewhere.

-20deg;C, protect from light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vitro:;

DMSO : 50 mg/mL (82.41 mM; Need ultrasonic)

H₂O : 8.33 mg/mL (13.73 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度，选择合适的溶剂配制储备液；该产品在溶液状态不稳定，建议您现用现配，即刻使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: 2.5 mg/mL (4.12 mM); Suspended solution; Need ultrasonic

  此方案可获得 2.5 mg/mL (4.12 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.5 mg/mL (4.12 mM); Clear solution

  此方案可获得 ≥ 2.5 mg/mL (4.12 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

• [1]. https://pubmed.ncbi.nlm.nih.gov/15782150/

  [2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9.

Acute brain slices are usually incubated in carbonated extracellular solution containing 0.5 to1 μM Sulforhodamine 101 for 20 to 30 min and 34 to 37°C. Following this, excess dye is removed over a period of 10 to 30 min using different protocols that were described earlier^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

For in vivo imaging, Sulforhodamine 101 (SR101) is applied topically at concentrations of 250 nM to 300 μM or by bolus injection. Additionally, Sulforhodamine 101 injection over the tail vein (10 mg/mL) has been reported to be successful^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. https://pubmed.ncbi.nlm.nih.gov/15782150/

  [2]. J Kang, et al. Sulforhodamine 101 induces long-term potentiation of intrinsic excitability and synaptic efficacy in hippocampal CA1 pyramidal neurons. Neuroscience. 2010 Sep 15;169(4):1601-9.