Theaflavin 3,3′-digallate(Synonyms: 茶黄素-3,3′-双没食子酸; TF-3; ZP10)

天然产物 黄酮类 Flavonoids

Theaflavin 3,3′-digallate (Synonyms: 茶黄素-3,3′-双没食子酸; TF-3; ZP10) 纯度: 99.73%

Theaflavin 3,3′-digallate (TF-3) 是有效的寨卡病毒 (ZIKV) 蛋白酶抑制剂,IC50 为 2.3 μM。Theaflavin 3,3′-digallate 直接与 ZIKVpro 结合 (Kd=8.86 µM) 并抑制 ZIKV 复制。Theaflavin 3,3′-digallate 抑制 gp41 和 NS2B-3 蛋白酶的活性,并具有抗 HSVHIV-1 病毒活性。Theaflavin 3,3′-digallate 是红茶中的典型色素,一种有效的抗肿瘤剂。

Theaflavin 3,3

Theaflavin 3,3′-digallate Chemical Structure

CAS No. : 30462-35-2

规格 价格 是否有货 数量
10 mM * 1 mL in DMSO ¥3278 In-stock
5 mg ¥2020 In-stock
10 mg ¥3430 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Theaflavin 3,3′-digallate 相关产品


  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Antiviral Compound Library
  • Phenols Library
  • Traditional Chinese Medicine Monomer Library
  • Flavonoids Library
  • Food-Sourced Compound Library


Theaflavin 3,3′-digallate (TF-3) is a potent Zika virus (ZIKV) protease inhibitor with an IC50 of 2.3 μM. Theaflavin 3,3′-digallat directly binds to ZIKVpro (Kd=8.86 µM) and inhibits ZIKV replication. Theaflavin 3,3′-digallat inhibits the activity of gp41 and NS2B-3 protease and has antiviral activity against HSV and HIV-1[1]. Theaflavin 3,3′-digallate, the typical pigment in black tea, is a potent antitumor agent[2].

IC50 & Target[1]





(In Vitro)

Theaflavin 3,3′-digallate (TF-3; 6.25, 12.5, 25 μM; 24 hours) markedly reduces viral RNA copy numbers and NS3, U87 MG protein expression in a dose-dependent manner[1].
Theaflavin 3,3′-digallate inhibits dose-dependently ZIKV replication in Vero E6 cells (EC50=7.65 μM). Theaflavin 3,3′-digallate has minor cytotoxicity up to 40 μM in Vero E6 cells. Theaflavin 3,3′-digallate can inhibit the post-entry events of the ZIKV replication cycle from gene transcription and translation levels[1].
Theaflavin 3,3′-digallate is generally regarded as the effective component for the inhibitory effects against carcinogenesis without adverse side effects by affecting multiple signal transduction pathways, such as upregulating p53 and p21, inhibiting phosphorylation of the cell survival protein Akt and MAPK pathway, downregulation of NF-κB, shifting the ratio between pro-/antiapoptotic proteins. Theaflavin 3,3′-digallate causes a rapid and sustained decrease in phospho-ERK1/2 and -MEK1/2 protein expression. Theaflavin 3,3′-digallate inhibits HCT116 cell growth with an IC50 of 17.26 μM[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: Vero E6 cells
Concentration: 6.25, 12.5, 25 μM
Incubation Time: 24 hours
Result: Markedly reduced NS3, U87 MG protein expression in a dose-dependent manner.

Clinical Trial










Room temperature in continental US; may vary elsewhere.


-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

In Vitro: 

DMSO : 100 mg/mL (115.11 mM; Need ultrasonic)

浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1511 mL 5.7557 mL 11.5115 mL
5 mM 0.2302 mL 1.1511 mL 2.3023 mL
10 mM 0.1151 mL 0.5756 mL 1.1511 mL


储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.88 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (2.88 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.88 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
  • [1]. Xiangling Cui, et al. Identification of Theaflavin-3,3′-Digallate as a Novel Zika Virus Protease Inhibitor. Front Pharmacol. 2020 Oct 21;11:514313.

    [2]. Ding Y, et al. Pre-treated theaflavin-3,3′-digallate has a higher inhibitory effect on the HCT116 cell line. Food Nutr Res. 2017 Nov 15;61(1):1400340.