Gap 27

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Gap 27; 纯度: 98.07%

Gap 27，一种合成的 connexin43 模拟肽，是一种间隙连接抑制剂。Gap 27 与通往第二跨膜连接蛋白区段的第二细胞外环的一部分具有保守的序列同源性。

Gap 27amp;;

Gap 27 Chemical Structure

CAS No. : 198284-64-9

规格 价格 是否有货 数量
1 mg ￥700 In-stock
5 mg ￥1300 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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Gap 27 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Gap 27, a synthetic connexin43 mimetic peptide, is a gap junction inhibitor. Gap 27 possesses conserved sequence homology to a portion of the second extracellular loop leading into the fourth transmembrane connexin segment[1][2].

体外研究
(In Vitro)

Gap 27 causes a remarked decrease in the number of both TRAP-positive mononuclear and multinucleated rat osteoclasts cultured on bovine bone slices. The activity of the remaining osteoclasts is found to be diminished by measuring the percentage of osteoclasts with actin rings of all TRAP-positive cells. In addition, the resorbed area in the treated cultures is greatly diminished[1]. Incubation of the carotid artery with the gap junction inhibitor Gap 27 (500 μM) essentially abolishes the hyperpolarization to acetylcholine but it is without effect on that to levcromakalim. In the guinea-pig isolated internal carotid artery, Gap 27 inhibits acetylcholine-induced, endothelium-dependent hyperpolarizations[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Gap 27 (300 μM) inhibits relaxation by 40% in thoracic aorta and the superior mesenteric artery. Gap 27 also attenuates the endothelium-dependent component of the relaxation induced by ATP in thoracic aorta. [3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1304.53

Formula

C60H101N15O17
CAS 号

198284-64-9
Sequence

Ser-Arg-Pro-Thr-Glu-Lys-Thr-Ile-Phe-Ile-Ile
Sequence Shortening

SRPTEKTIFII
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 33.33 mg/mL (25.55 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.7666 mL 3.8328 mL 7.6656 mL
5 mM 0.1533 mL 0.7666 mL 1.5331 mL
10 mM 0.0767 mL 0.3833 mL 0.7666 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Ilvesaro J, et al. Connexin-mimetic peptide Gap 27 decreases osteoclastic activity. BMC Musculoskelet Disord. 2001;2:10.

    [2]. Edwards G, et al. Role of gap junctions in the responses to EDHF in rat and guinea-pig small arteries. Br J Pharmacol. 1999 Dec;128(8):1788-94.

    [3]. Chaytor AT,e t al. Central role of heterocellular gap junctional communication in endothelium-dependent relaxations of rabbit arteries. J Physiol. 1998 Apr 15;508 ( Pt 2):561-73.
Cell Assay
[1]

Bone cell cultures are cultured for 48 hours with three different treatments (control, heptanol and Gap 27). After the culture period, bone slices are fixed. The cells are stained for tartrate-resistant acid phosphatase (TRAP). To visualise the nuclei, the cells are incubated with the DNA-binding fluorochrome Hoechst 33258 (1 mg/mL stock diluted 1:800 in PBS) for 10 minutes at room temperature. The numbers of mononuclear and multinucleated TRAP-positive cells on each bone slice are counted[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Ilvesaro J, et al. Connexin-mimetic peptide Gap 27 decreases osteoclastic activity. BMC Musculoskelet Disord. 2001;2:10.

    [2]. Edwards G, et al. Role of gap junctions in the responses to EDHF in rat and guinea-pig small arteries. Br J Pharmacol. 1999 Dec;128(8):1788-94.

    [3]. Chaytor AT,e t al. Central role of heterocellular gap junctional communication in endothelium-dependent relaxations of rabbit arteries. J Physiol. 1998 Apr 15;508 ( Pt 2):561-73.