Punicalagin(Synonyms: 安石榴甙)

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

Punicalagin (Synonyms: 安石榴甙) 纯度: 99.80%

Punicalagin 是一种从 Punica granatum L. 或 Terminalia catappa L. 的叶子中分离的多酚成分。Punicalagin 是一种可逆且非竞争性的 3CLpro 抑制剂,在体外可抑制 SARS-CoV-2 复制。Punicalagin 是一种抗乙型肝炎病毒 (HBV) 药物,具有抗氧化,抗炎和抗癌作用。Punicalagin 有潜力用于 COVID-19 的研究。

Punicalagin(Synonyms: 安石榴甙)

Punicalagin Chemical Structure

CAS No. : 65995-63-3

规格 价格 是否有货 数量
5 mg ¥500 In-stock
10 mg ¥900 In-stock
20 mg ¥1500 In-stock
50 mg   询价  
100 mg   询价  

* Please select Quantity before adding items.

Punicalagin 相关产品

相关化合物库:

  • Covalent Screening Library Plus
  • Natural Product Library Plus
  • Drug Repurposing Compound Library Plus
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Natural Product Library
  • Anti-Cancer Compound Library
  • Clinical Compound Library
  • Antiviral Compound Library
  • Drug Repurposing Compound Library
  • Covalent Screening Library
  • Terpenoids Library
  • Phenols Library
  • Traditional Chinese Medicine Monomer Library
  • Food-Sourced Compound Library

生物活性

Punicalagin is a polyphenol ingredient isolated from Pomegranate (Punica granatum L.) or the leaves of Terminalia catappa L.. Punicalagin is a reversible and non-competitive 3CLpro inhibitor and inhibits SARS-CoV-2 replication in vitro. Punicalagin is an anti-hepatitis B virus (HBV) agent and has antioxidant, anti-inflammatory, and anticancer effects. Punicalagin has the potential for the research of COVID-19[1][2][3].

体外研究
(In Vitro)

Punicalagin (100 mg/ml) induces apoptosis in HT-29, HCT116 colon cells[1].
Punicalagin slightly inhibits the PLpro activity with an IC50 of over 50 μM. Punicalagin inhibits the plaque formation of SARS-CoV-2 in a dose-dependent manner, with EC50 values of 7.20 μM[4].
Punicalagin binds at an allosteric site in the dimer interface. Punicalagin inhibit SARS-CoV-2 replication by a mechanism other than preventing S-mediated viral entry[4].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

Punicalagin (10 mg/kg) inhibits the edema rate of 58.15% in rats[2].

Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

分子量

1084.72

Formula

C48H28O30

CAS 号

65995-63-3

中文名称

安石榴甙

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO : 50 mg/mL (46.09 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.9219 mL 4.6095 mL 9.2190 mL
5 mM 0.1844 mL 0.9219 mL 1.8438 mL
10 mM 0.0922 mL 0.4609 mL 0.9219 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂: 10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.17 mg/mL (2.00 mM); Clear solution

    此方案可获得 ≥ 2.17 mg/mL (2.00 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂: 10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.17 mg/mL (2.00 mM); Clear solution

    此方案可获得 ≥ 2.17 mg/mL (2.00 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
*以上所有助溶剂都可在 Shanghai Jinpan Biotech Co Ltd 网站选购。
参考文献
  • [1]. Seeram NP, et al. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagicacid and a total pomegranate tannin extract are enhanced in combination with otherpolyphenols as found in pomegranate juice. J Nutr Biochem. 2005 Jun;16(6):360-7.

    [2]. Lin CC, et al. Effects of punicalagin and punicalin on carrageenan-induced inflammation in rats. Am J Chin Med. 1999;27(3-4):371-6.

    [3]. Liu C, et al. Identification of hydrolyzable tannins (punicalagin, punicalin and geraniin) as novel inhibitors of hepatitis B virus covalently closed circular DNA. Antiviral Res. 2016 Oct;134:97-107.

    [4]. RuikunDu, et al. Discovery of Chebulagic Acid and Punicalagin as Novel Allosteric Inhibitors of SARS-CoV-2 3CLpro. Antivir Res. 2021, 105075.