CREBtide

发表于由

CREBtide; 纯度: 98.89%

CREBtide 是一种合成的含 13 个氨基酸的肽，作为一种 PKA 底物。

CREBtideamp;;

CREBtide Chemical Structure

CAS No. : 149155-45-3

规格 价格 是否有货 数量
1 mg ￥800 In-stock
5 mg ￥2800 In-stock
10 mg ￥4400 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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CREBtide 相关产品

bull;相关化合物库:

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  • Kinase Inhibitor Library
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  • Peptide Library

生物活性

CREBtide, a synthetic 13 amino acid peptide, has been reported as a PKA substrate.

IC50 Target

PKA[1]
体外研究
(In Vitro)

delta-CREB is a spliced variant of cAMP response element binding protein (CREB). CREBtide (KRREILSRRPSYR), a synthetic peptide based on the phosphorylation sequence in delta-CREB. delta-CREB and CREBtide are tested as substrates of cAMP-dependent protein kinase (cAK). The apparent Km of CREBtide phosphorylation by cAK is 3.9 μM, which is 10-fold lower than that of Kemptide (Km=39 μM), the synthetic peptide substrate most often employed for cAK measurement. The Vmax values are 12.4 mumol/(min.mg) for CREBtide and 9.8 mumol/(min.mg) for Kemptide. The apparent Km of CREBtide phosphorylation by cGMP-dependent protein kinase (cGK) is 2.9 μM and the Vmax value is 3.2 mumol/(min.mg). Both delta-CREB and CREBtide are phosphorylated at a much slower rate by cGK as compared with cAK, implying that the high cAK/cGK specificity exhibits by delta-CREB is retained by the peptide[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1716.99

Formula

C73H129N29O19
CAS 号

149155-45-3
Sequence

Lys-Arg-Arg-Glu-Ile-Leu-Ser-Arg-Arg-Pro-Ser-Tyr-Arg
Sequence Shortening

KRREILSRRPSYR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 100 mg/mL (58.24 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.5824 mL 2.9121 mL 5.8241 mL
5 mM 0.1165 mL 0.5824 mL 1.1648 mL
10 mM 0.0582 mL 0.2912 mL 0.5824 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Wu J, et al. A microPLC-based approach for determining kinase-substrate specificity. Assay Drug Dev Technol. 2007 Aug;5(4):559-66.

    [2]. Colbran JL, et al. cAMP-dependent protein kinase, but not the cGMP-dependent enzyme, rapidly phosphorylates delta-CREB, and a synthetic delta-CREB peptide. Biochem Cell Biol. 1992 Oct-Nov;70(10-11):1277-82.