天然产物 黄酮类 Flavonoids
Phellamurin
Phellamurin 是一种来自 Phellodendron amurense 叶的植物黄酮糖苷，可抑制肠道 P-糖蛋白 (P-glycoprotein)。Phellamurin 还抑制凤蝶的产卵。Phellamurin 诱导细胞凋亡 (apoptosis)，并具有抗肿瘤活性。
Phellamurin Chemical Structure
CAS No. : 52589-11-4
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| 生物活性 |
Phellamurin is a plant flavonone glycoside from the leaves of Phellodendron amurense and inhibits intestinal P-glycoprotein. Phellamurin also inhibits egg laying by Papilio protenor. Phellamurin induces cells apoptosis and has anti-tumor activity[1][2][3].
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体外研究
(In Vitro) |
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment leads to a repression of cell viability in U2OS and Saos-2 cells in a dose-dependent manner[1].
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment concentration-dependently promots the apoptosis of U2OS and Saos-2 cells[1].
Phellamurin (0-10 μg/mL; 48 hours; U2OS and Saos-2 cells) treatment declines the ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR in U2OS and Saos-2 cells[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
Cell Viability Assay[1]
| Cell Line: |
U2OS and Saos-2 cells |
| Concentration: |
0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL |
| Incubation Time: |
48 hours |
| Result: |
Suppressed the viability of U2OS and Saos-2 cells in a concentration-dependent manner. |
Apoptosis Analysis[1]
| Cell Line: |
U2OS and Saos-2 cells |
| Concentration: |
0 μg/mL, 2.5 μg/mL, 5 μg/mL, and 10 μg/mL |
| Incubation Time: |
48 hours |
| Result: |
Induced apoptosis of U2OS and Saos-2 cells in a concentration-dependent manner. |
Western Blot Analysis[1]
| Cell Line: |
U2OS and Saos-2 cells |
| Concentration: |
0 μg/mL and 10 μg/mL |
| Incubation Time: |
48 hours |
| Result: |
Repressed the PI3K/AKT/mTOR pathway in U2OS and Saos-2 cells. |
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体内研究
(In Vivo) |
Phellamurin (50 mg/kg/day; intraperitoneal injection; daily; for 21 days; female BALB/c nude mice) treatment represses osteosarcoma tumor growth in vivo. The ratios of p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR are decreased in xenograft in Phellamurin-treated mice[1].
Shanghai Jinpan Biotech Co Ltd has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: |
Athymic female BALB/c nude mice (6 weeks old ) injected with U2OS cells[1] |
| Dosage: |
50 mg/kg/day |
| Administration: |
Intraperitoneal injection; daily; for 21 days |
| Result: |
Repressed osteosarcoma tumor growth in vivo. |
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| 分子量 |
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| Formula |
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| CAS 号 |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
| Powder |
-20°C |
3 years |
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4°C |
2 years |
| In solvent |
-80°C |
6 months |
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-20°C |
1 month |
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| 参考文献 |
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[1]. Hongzhi Zhang, et al. Anti-tumor Efficacy of Phellamurin in Osteosarcoma Cells: Involvement of the PI3K/AKT/mTOR Pathway. Eur J Pharmacol. 2019 Sep 5;858:172477.
[2]. Hung-Yi Chen, et al. Marked Decrease of Cyclosporin Absorption Caused by Phellamurin in Rats. Planta Med. 2002 Feb;68(2):138-41.
[3]. Keiichi Honda, et al. Synergistic or Antagonistic Modulation of Oviposition Response of Two Swallowtail Butterflies, Papilio Maackii and P. Protenor, to Phellodendron Amurense by Its Constitutive Prenylated Flavonoid, Phellamurin. J Chem Ecol. 2011 Jun;37(6):575-81.
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