D-3

D-3;

D-3 是一种磷酸化肽,是一种高效、简单、特异的 IPSC 消除剂。

D-3amp;;

D-3 Chemical Structure

CAS No. : 1967815-98-0

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生物活性

D-3, a phosphorpeptide, is an efficient, simple, and specific iPSC-eliminating agent[1].

体外研究
(In Vitro)

D-3 prevents residual iPSC-induced teratoma formation in a mouse tumorigenicity assay[1].
D-3 induces obvious loss of viability in 201B7 cells, with half maximal inhibitory concentration value of 192.3 ± 57.4 μM[1].
D-3 induces an increase in the amount of activated elF2a, p38, and p44/42 MAPK, which are activated in response to cellular stress. D-3 also increases the number of Annexin-V and SYTOX-positive cells, indicating apoptotic and dead cells after D-3 treatment[1].
D-3 has little influence on various non-iPSCs, including hepatocytes and neurons[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: Six human iPSCs lines and one human ESC line (khES1).
Concentration: 400 μM.
Incubation Time: 1-2 h.
Result: Sufficient to induce a viability loss (>99% in all iPSC lines and >95% in khES1).

Clinical Trial

分子量

870.88

Formula

C48H47N4O10P

CAS 号

1967815-98-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Yi Kuang, et al. Efficient, Selective Removal of Human Pluripotent Stem Cells via Ecto-Alkaline Phosphatase-Mediated Aggregation of Synthetic Peptides. Cell Chem Biol. 2017 Jun 22;24(6):685-694.e4.

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