Orexin B, rat, mouse TFA(Synonyms: Rat orexin B TFA Orexin B (mouse) (TFA))

发表于由

Orexin B, rat, mouse TFA;(Synonyms: Rat orexin B TFA; Orexin B (mouse) (TFA)) 纯度: 98.49%

Orexin B, rat, mouse (Rat orexin B) TFA 是一种内源性增食欲素受体 (Orexin receptor) 激动剂。Orexin B, rat, mouse TFA 结合并激活两个密切相关的孤儿 G 蛋白偶联受体 OX1-R 和 OX2-R。Orexin B, rat, mouse TFA 刺激食物摄入和能量消耗，并在睡眠-觉醒调节中发挥重要作用。

Orexin B, rat, mouse TFAamp;;(Synonyms: Rat orexin B TFA; Orexin B (mouse) (TFA))

Orexin B, rat, mouse TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥3000 In-stock
5 mg ￥7500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Orexin B, rat, mouse (Rat orexin B) TFA is an endogenous orexin receptor agonist. Orexin B, rat, mouse TFA binds and activates two closely related orphan G protein-coupled receptors OX1-R and OX2-R. Orexin B, rat, mouse TFA stimulates food intake and energy expenditure and plays a significant role in sleep-wakefulness regulation[1][2][3].

体外研究
(In Vitro)

OX2 receptor is indeed a high-affinity receptor for human orexin B, with an IC50 of 36 nM in the binding assay and an EC50 of 60 nM in the [Ca2+]i transient assay. Human Orexin B has significantly lower affinity for the human OX1: the calculated IC50 in the competitive binding assay and the EC50 in the [Ca2+]i transient assay are 420 nM and 2500 nM for human orexin-B, respectively[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Orexin-B modulates the activity of rod bipolar cells (RBCs) located in the outer retina of rat. Intravitreal injection of orexin-B increased the amplitude of the scotopic electroretinographic b-wave, a reflection of RBC activity, recorded in vivo[4].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3050.42

Formula

C₁₂₈H₂₁₆F₃N₄₅O₃₆S
Sequence Shortening

RPGPPGLQGRLQRLLQANGNHAAGILTM-NH2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (32.78 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.3278 mL 1.6391 mL 3.2782 mL
5 mM 0.0656 mL 0.3278 mL 0.6556 mL
10 mM 0.0328 mL 0.1639 mL 0.3278 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Smart D, et al. Orexins: a new family of neuropeptides. Br J Anaesth. 1999 Nov;83(5):695-7.

    [2]. Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85.

    [3]. Ziolkowska A, et al. Orexin B inhibits proliferation and stimulates specialized function of cultured rat calvarial osteoblast-like cells. Int J Mol Med. 2008;22(6):749-755.

    [4]. Zhang G, et al. Orexin-B modulates synaptic transmission of rod bipolar cells in rat retina. Neuropharmacology. 2018;133:38-50.