标签归档：rat

Bio-Serv动物模型饲料MD300-2 Rat MD’s, Baytril (2 mg/Tablet)Bacon Flavor, 100/Bottle

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Bio-Serv动物模型饲料MD300-2 Rat MD’s, Baytril (2 mg/Tablet)Bacon Flavor, 100/Bottle

MD300-2

F05266

F07813

Product Details

5 gm Tablet – 1″ dia. x 1/4″ high
Shelf Life: 6 months, cool dry storage

Baytril is an effective antibiotic drug used in a variety of species including rodents. Baytril MD’s are highly palatable and encourage appetite post-operatively promoting a more rapid recovery versus other conventional methods. They are an ideal pain-free alternative to stressful and injurious post-operative injections. Baytril MD’s can be customized to include anti-inflammatory drugs such as Rimadyl and Meloxicam to provide both medications easily and affordably.

  • Highly palatable, nutritionally complete, grain-based, bacon flavored tablets
  • Easy to administer: Place on cage floor for easy access
  • Each 5 gram bacon-flavored tablet contains: 2 mg Baytril
  • Rat Average Daily Dose: One 5 gram Tablet/Rat per day provides daily medication needs and is a dietary supplement to standard feed
  • Introduction of Bio-Serv’s Placebo or MD tablet 1-2 days prior to surgery will decrease novelty and increase acceptance
  • More convenient and less risk of dehydration compared to using medicated water
  • Safe alternative to traditional dosing methods
  • Cold tableting process eliminates drug degradation
  • Can be customized to meet specific dosage requirements
  • Nutritionally Assayed

Gamma irradiation is available upon request. Please contact Customer Service for pricing and lead time.

Nutritional Profile
Protein
Fat
Fiber		 21.3
4.3
4.0		 %
%
%		 Ash
Moisture
Carbohydrate		 7.8
<10
54.0		 %
%
%
Calories: 3.39 kcal/gm

MD300-2 Rat MD  MD300-2 Nutritional Profile
  F05266 Nutritional Profile
        F07813 Nutritional Profile

Ingredients
Ground Corn, Banana Flakes, Dehulled Soybean Meal, Corn Gluten Meal, Ground Wheat, Corn Gluten Feed, Fish Meal, Dehydrated Alfalfa Meal, Casein, Dried Whey, Sucrose, Fructose, Dextrose, Dried Beet Pulp, Soybean Oil, Porcine Animal Fat (Preserved with BHA), Dried Brewers Yeast, Mineral Mix, Vitamin Mix, Magnesium Stearate, Calcium Silicate, Bacon Flavor, DL-Methionine, Choline Chloride, Sodium Propionate, Ascorbic Acid, Kaolin

Feeding Guidelines

For best acceptance, introduce Bio-Serv’s placebo or MD Tablets 1-2 days prior to surgery. Feed one MD tablet per rat per day to provide the daily medication dose and nutritional supplement. Place tablets at cage floor level for ease of access by the animal. Standard feed should remain in feed hopper.

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Bio-Serv动物模型饲料K3325 Rat Tunnel, Red, Certified

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上海金畔生物科技有限公司代理Bio-Serv品牌动物模型饲料全线产品，欢迎访问官网了解更多产品信息。
Bio-Serv动物模型饲料K3325 Rat Tunnel, Red, Certified

K3325

K3326

K3339

Product Details
  • Certified (Contaminant Screened)
  • Made of high temp polycarbonate
  • Offers rodents shelter and security
  • Transparency makes routine cage checks
    easy and fast
  • Helps reduce boredom and aggressive behaivor
  • To increase physical activity and usable floor space,
    suspend with the optional stainless steel hanger
  • Autoclavable
  • Cagewasher safe
Dimensions
Tunnels – 6″ long x 3″ inside diameter; 1/8″ wall
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Bio-Serv动物模型饲料K3170 Rat Retreat, Blue, Certified

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上海金畔生物科技有限公司代理Bio-Serv品牌动物模型饲料全线产品，欢迎访问官网了解更多产品信息。
Bio-Serv动物模型饲料K3170 Rat Retreat, Blue, Certified

K3170

K3245

K3246

Product Details
  • Certified (Contaminant Screened)
  • Made of high temp polycarbonate
  • Best rat enrichment ever!
  • Allows for sheltering and gnawing opportunities
  • Flat top allows for increased floor space
  • Large flat sides prevent rolling
  • Autoclavable
  • Cagewasher safe
Dimensions
3 3/4″ wide x 3 3/4″ high x 6″ long
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Orexin A (human, rat, mouse) (TFA)

发表于由

Orexin A (human, rat, mouse) (TFA); 纯度: 99.15%

Orexin A human, rat, mouse TFA 是一种 33 氨基酸兴奋性神经肽，参与多种中枢和外周过程。Orexin A human, rat, mouse TFA 是一种特异性、高亲和力的 G 蛋白偶联受体 OX1R 激动剂，在调节摄食行为中起重要作用。Orexin A human, rat, mouse TFA 是一种有效的抗伤害和抗痛觉过敏的药物。

Orexin A (human, rat, mouse) (TFA)amp;;

Orexin A (human, rat, mouse) (TFA) Chemical Structure

规格 价格 是否有货 数量
10;mM;*;1 mL in Water ￥145535 In-stock
1 mg ￥4400 In-stock
5 mg ￥18000 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Orexin A (human, rat, mouse) (TFA) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Orexin A human, rat, mouse TFA, a 33 amino acid excitatory neuropeptide, orchestrates diverse central and peripheral processes. Orexin A human, rat, mouse TFA is a specific, high-affinity agonist for G-protein-coupled receptor OX1R. Orexin A human, rat, mouse TFA has a role in the regulation of feeding behavior. Orexin A human, rat, mouse TFA is an effective anti-nociceptive and anti-hyperalgesic agent in mice and rats[1][2].

体外研究
(In Vitro)

Orexin A human, rat, mouse TFA has high affinity for OX1R, with 38 nM IC50 and 34 nM EC50 values in the the [Ca2﹢]i transient assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Orexin A human, rat, mouse TFA (3-30 mg/kg; i.v.; 5 min pre-test) significantly increases the latency to response at 10 and 30 mg/kg i.v. when given 5 min pre-test from 24.8±2.0 s in vehicle-treated mice to 35.0±3.7 s and 45.7±4.5 s, respectively[2].
Orexin A human, rat, mouse TFA (3, 10 and 30 mg/kg; i.v.) is given immediately before phenylp-quinone (PPQ) and increases the latency to the first PPQ-induced constriction from 357.4±35.2 s in vehicle-treated mice to 500.3±31.2 s at 10 mg/kg and 594.5±5.5 s at 30 mg/kg[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female mice (mouse carrageenan-induced thermal hyperalgesia test[2]
Dosage: 3, 10 and 30 mg/kg
Administration: i.v.; 5 min pre-test
Result: Significantly increased the latency to response at 10 and 30 mg/kg.

分子量

3675.12

Formula

C2HF3O2
Sequence

{Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-IIE-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
Sequence Shortening

{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (27.21 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2721 mL 1.3605 mL 2.7210 mL
5 mM 0.0544 mL 0.2721 mL 0.5442 mL
10 mM 0.0272 mL 0.1360 mL 0.2721 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85.

    [2]. Bingham S, et al. Orexin-A, an hypothalamic peptide with analgesic properties. Pain. 2001 May;92(1-2):81-90.

Brain Natriuretic Peptide (1-32), rat acetate(Synonyms: BNP (1-32), rat acetate)

发表于由

Brain Natriuretic Peptide (1-32), rat acetate;(Synonyms: BNP (1-32), rat acetate) 纯度: 99.66%

Brain Natriuretic Peptide (1-32), rat acetate (BNP (1-32), rat acetate) 是一种含有 32 个氨基酸的多肽。可作为心力衰竭病人病残率和死亡率的生化标志。

Brain Natriuretic Peptide (1-32), rat acetateamp;;(Synonyms: BNP (1-32), rat acetate)

Brain Natriuretic Peptide (1-32), rat acetate Chemical Structure

规格 价格 是否有货 数量
1 mg ￥4000 In-stock
5 mg ￥10000 In-stock
10 mg ￥15000 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Brain Natriuretic Peptide (1-32), rat acetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Brain Natriuretic Peptide (1-32), rat acetate (BNP (1-32), rat acetate) is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of heart muscle cells (cardiomyocytes)[1].

体外研究
(In Vitro)

B-type natriuretic peptide (BNP) combats cardiac stress by reducing blood pressure and ventricular fibrosis. Rat BNP BNP (1-32) (rBNP (1-32)) is an amino-truncated form of the 45 residue natural rat form of BNP[1]. Atrial natriuretic peptide-(1-28) (ANP), brain natriuretic peptide-(1-32) (BNP) and C-Type natriuretic polypeptide (CNP) occur in the brain, are concentrated in the anteroventral area of the third cerebral ventricle and participate in the regulation of body fluid homeostasis. The ANP(1-28), BNP (1-32) and CNP(1-32) function in the mammalian brain to regulate salt and water homeostasis via their receptors NPR-A and NPR-B[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

The depressor, natriuretic and cyclic GMP responses to several species of brain natriuretic peptide (BNP) are compared to atrial natriureticpeptide (ANP) 99-126 in conscious spontaneously hypertensive rats (SHR) and in conscious cynomolgus monkeys treated with vehicle or the selective neutral endopeptidase inhibitor SQ 28603. In the conscious SHR, the natriuretic and cyclic GMP responses to 3 nmol/kg i.v. rat BNP (1-32) greater than rat ANP 99-126 greater than pig BNP-26 and sre significantly potentiated by 100 mumol/kg i.v. SQ 28,603. Human BNP-32 is inactive in the SHR treated with either vehicle or SQ 28,603. In contrast, 1 nmol/kg i.v. of human BNP (1-32) stimulates renal and depressor responses in the conscious monkeys that are greater than or equal to those elicited by human ANP 99-126, whereas 3 nmol/kg i.v. rat BNP (1-32) reduces mean arterial pressure without affecting renal function[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3512.99

Formula

C148H243N47O46S3
Sequence

Asn-Ser-Lys-Met-Ala-His-Ser-Ser-Ser-Cys-Phe-Gly-Gln-Lys-Ile-Asp-Arg-Ile-Gly-Ala-Val-Ser-Arg-Leu-Gly-Cys-Asp-Gly-Leu-Arg-Leu-Phe (Disulfide bridge: Cys10-Cys26)
Sequence Shortening

NSKMAHSSSCFGQKIDRIGAVSRLGCDGLRLF (Disulfide bridge: Cys10-Cys26)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Dickey DM, et al. Human B-type natriuretic peptide is not degraded by meprin A. Biochem Pharmacol. 2010 Oct 1;80(7):1007-11.

    [2]. Wellard J, et al. Natriuretic peptides, but not nitric oxide donors, elevate levels of cytosolic guanosine 3′,5′-cyclic monophosphate in ependymal cells ex vivo. Neurosci Lett. 2006 Jan 16;392(3):187-92.

    [3]. Seymour AA, et al. Potentiation of brain natriuretic peptides by SQ 28,603, an inhibitor of neutral endopeptidase3.4.24.11, in monkeys and rats. J Pharmacol Exp Ther. 1992 Jul;262(1):60-70.

Bio-Serv动物模型饲料9004 Rat Pellet Tube

发表于由

上海金畔生物科技有限公司代理Bio-Serv品牌动物模型饲料全线产品，欢迎访问官网了解更多产品信息。
Bio-Serv动物模型饲料9004 Rat Pellet Tube

9004

9005

90045

90055

Product Details
Tubes:

  • Made of borosilicate glass
  • Easy, accurate and convenient method
    of measuring food intake
  • Narrow opening allows access to pellet
    without removal from the tube
  • Mouse tubes hold eight 1 gm pellets
  • Rat tubes hold eleven 1 gm pellets
  • Autoclavable
  • Cagewasher safe

Holders:

  • Made of stainless steel
  • Bottom tray catches any reclaimable pellet fines
  • Holder is designed to hang over lip of cage
  • Autoclavable
  • Cagewasher safe
Dimensions
Mouse Feeding Tube – 3 1/2″ tall x 1/2″ od
Mouse Tube Holder – 4 1/2″ tall x 1 3/8 " wide

Rat Feeding Tube – 5 1/4″ tall x 1/2″ od
Rat Tube Holder – 5 3/4″ tall x 1 3/8 " wide

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Endothelin-3, human, mouse, rabbit, rat TFA(Synonyms: Endothelin 3 (Rat,Human) (TFA))

发表于由

Endothelin-3, human, mouse, rabbit, rat TFA;(Synonyms: Endothelin 3 (Rat,Human) (TFA)) 纯度: 99.66%

Endothelin-3, human, mouse, rabbit, rat TFA 是由21个氨基酸组成的血管活性肽，可与G蛋白连接的跨膜受体，ET-RA 和ET-RB 结合。

Endothelin-3, human, mouse, rabbit, rat TFA&nbsp;(Synonyms: Endothelin 3 (Rat,Human) (TFA))

Endothelin-3, human, mouse, rabbit, rat TFA Chemical Structure

规格 价格 是否有货 数量
500 μg ￥1800 In-stock
1 mg ￥2800 In-stock
5 mg ￥9800 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

Endothelin-3, human, mouse, rabbit, rat TFA 相关产品

•相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Endothelin-3, human, mouse, rabbit, rat TFA is a 21-amino acid vasoactive peptide that binds to G-protein-linked transmembrane receptors, ET-RA and ET-RB.

IC50 & Target

ET-RA, ET-RB[1]
分子量

2757.07

Formula

C123H169F3N26O35S4
Sequence

Cys-Thr-Cys-Phe-Thr-Tyr-Lys-Asp-Lys-Glu-Cys-Val-Tyr-Tyr-Cys-His-Leu-Asp-Ile-Ile-Trp(Disulfide bridge: Cys1-Cys15, Cys3-Cys11)
Sequence Shortening

CTCFTYKDKECVYYCHLDIIW(Disulfide bridge: Cys1-Cys15, Cys3-Cys11)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (<50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Skovsted GF, et al. Endothelin-1 and Endothelin-3 Regulate Endothelin Receptor Expression in Rat Coronary Arteries. Basic Clin Pharmacol Toxicol. 2015 Nov;117(5):297-305.

Orexin A (human, rat, mouse)

发表于由

Orexin A (human, rat, mouse);

Orexin A human, rat, mouse 是一种 33 氨基酸兴奋性神经肽，参与多种中枢和外周过程。Orexin A human, rat, mouse 是一种特异性、高亲和力的 G 蛋白偶联受体 OX1R 激动剂，在调节摄食行为中起重要作用。Orexin A human, rat, mouse 是一种有效的抗伤害和抗痛觉过敏的药物。

Orexin A (human, rat, mouse)&nbsp;

Orexin A (human, rat, mouse) Chemical Structure

CAS No. : 205640-90-0

规格 价格 是否有货
1 mg ￥4400 询问价格 & 货期
5 mg ￥18000 询问价格 & 货期

* Please select Quantity before adding items.

Orexin A (human, rat, mouse) 的其他形式现货产品:

Orexin A (human, rat, mouse) (TFA)

生物活性

Orexin A human, rat, mouse, a 33 amino acid excitatory neuropeptide, orchestrates diverse central and peripheral processes. Orexin A human, rat, mouse is a specific, high-affinity agonist for G-protein-coupled receptor OX1R. Orexin A human, rat, mouse has a role in the regulation of feeding behavior. Orexin A human, rat, mouse is an effective anti-nociceptive and anti-hyperalgesic agent in mice and rats[1][2].

体外研究
(In Vitro)

Orexin A human, rat, mouse has high affinity for OX1R, with 38 nM IC50 and 34 nM EC50 values in the the [Ca2﹢]i transient assay[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Orexin A human, rat, mouse (3-30 mg/kg; i.v.; 5 min pre-test) significantly increases the latency to response at 10 and 30 mg/kg i.v. when given 5 min pre-test from 24.8±2.0 s in vehicle-treated mice to 35.0±3.7 s and 45.7±4.5 s, respectively[2].
Orexin A human, rat, mouse (3, 10 and 30 mg/kg; i.v.) was given immediately before phenylp-quinone (PPQ) and increases the latency to the first PPQ-induced constriction from 357.4±35.2 s in vehicle-treated mice to 500.3±31.2 s at 10 mg/kg and 594.5±5.5 s at 30 mg/kg[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Female mice (mouse carrageenan-induced thermal hyperalgesia test)[2]
Dosage: 3, 10 and 30 mg/kg
Administration: i.v.; 5 min pre-test
Result: Significantly increased the latency to response at 10 and 30 mg/kg.

分子量

3561.10

Formula

C152H243N47O44S4
CAS 号

205640-90-0
Sequence

{Glp}-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu-Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-IIE-Leu-Thr-Leu-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
Sequence Shortening

{Glp}-PLPDCCRQKTCSCRLYELLHGAGNHAAGILTL-NH2 (Disulfide bridge: Cys6-Cys12, Cys7-Cys14)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (<50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Sakurai T, et al. Orexins and orexin receptors: a family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior. Cell. 1998 Feb 20;92(4):573-85.

    [2]. Bingham S, et al. Orexin-A, an hypothalamic peptide with analgesic properties. Pain. 2001 May;92(1-2):81-90.

Ames Test-Rat liver 9,000×g supernatant fraction S-9 大鼠肝匀浆

发表于由

Ames Test-Rat liver 9,000×g supernatant fraction
S-9 大鼠肝匀浆

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

S-9 大鼠肝匀浆/致突变性试验用（Ames试验用）Ames Test-Rat liver 9,000×g supernatant fraction S-9 大鼠肝匀浆

Ames Test-Rat liver 9,000×g supernatant fraction

◆「S-9」调配方法

　　将雄性大鼠饲养与 SPF 环境下，养至７周大小（体重180 g-200 g）后，把诱导剂 Phenobarbital（PB）和 5, 6-Benzoflavone（BF）投与大鼠肝脏。

　　PB 是在剖杀前４天连续每天投注，BF 则是剖杀前２天投注一次。然后无菌摘取大鼠肝脏，匀浆后 9000×g 离心，收集 S-9 上清。调配方法以 Ames 方法为准。



附上每个生产批次的生化特性检测值，如下：


生化特性


类型

检测项目

S-9

蛋白质含量

微粒体

(105,000×g）

 

蛋白质含量

Cytochrome P-450 含量

Cytochrome β5 含量

NADP-Cytochrome reductase

Aniline hydroxylase

附上每个生产批次的突变诱发性的生物活性检测值，如下：

 

生化活性


药剂

菌株

Benzo（α）pyrene

TA100
TA98
TA1537

2-Aminoanthracene

TA100
TA98

形态/保存

　　到货后请直接保存于-80℃以下。

　　干冰泡沫箱包装

◆相关链接


　　Ames Test概要

产品列表
产品编号 产品名称 产品规格 产品等级 备注
636-00221 Ames Test-Rat liver 9,000×g supernatant fraction 2 mL×10 – –

β-Amyloid (1-40) (rat)(Synonyms: β淀粉样肽(1-40)(鼠))

发表于由

β-Amyloid (1-40) (rat);(Synonyms: β淀粉样肽(1-40)(鼠))

β-Amyloid (1-40) (rat) 是蛋白水解切割淀粉样蛋白前体蛋白的易聚集产物，可以用于阿尔茨海默病的研究。

β-Amyloid (1-40) (rat)amp;;(Synonyms: β淀粉样肽(1-40)(鼠))

β-Amyloid (1-40) (rat) Chemical Structure

CAS No. : 144409-98-3

规格 价格 是否有货
1 mg ￥2200 询问价格 货期

* Please select Quantity before adding items.

生物活性

β-Amyloid (1-40) (rat) is the prone-to-aggregation product of amyloid precursor protein proteolytic cleavage, and can be used for the research of Alzheimer’s disease[1].

分子量

4233.76

Formula

C190H291N51O57S
CAS 号

144409-98-3
Sequence

Asp-Ala-Glu-Phe-Gly-His-Asp-Ser-Gly-Phe-Glu-Val-Arg-His-Gln-Lys-Leu-Val-Phe-Phe-Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val
Sequence Shortening

DAEFGHDSGFEVRHQKLVFFAEDVGSNKGAIIGLMVGGVV
中文名称

β淀粉样肽(1-40)(鼠)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Shi X, et al. Rat hippocampal proteomic alterations following intrahippocampal injection of amyloid betapeptide (1-40). Neurosci Lett. 2011 Aug 15;500(2):87-91.
Animal Administration

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Shi X, et al. Rat hippocampal proteomic alterations following intrahippocampal injection of amyloid betapeptide (1-40). Neurosci Lett. 2011 Aug 15;500(2):87-91.