标签归档：violet

结晶紫染液

发表于由

中文别名：甲基紫10B;甲紫;六甲基紫;六甲基玫苯胺盐酸盐;盐基紫3

英文名称：Crystal violet – Solution

英文别名：Gentian violet, Hexamethylene pararoseaniline chloride, Methyl violet 10 B, Pyoctaninum coeruleum 

CAS号：548-62-9

分子式：C25H30ClN3

分子量：407.99

存储温度：常温

含量：0.5%

性状：结晶紫是碱性染料，绿色带有金属光泽结晶或深绿色结晶性粉末，为副品红的六甲基衍生物，含有少量四、五甲基的衍生物；1g溶于10ml乙醇、15ml甘油，溶于水和氯仿，水溶液和乙醇液呈紫色，几乎不溶于乙醚。

用途：结晶紫在细胞学、组织学和细菌学等方面应用极广，是一种优良的染色剂。结晶紫可以和细胞核中的DNA结合，是细胞核染色常用的，用来显示染色体的中心体，并可染淀粉、纤维蛋白、神经胶质等。凡是用番红和苏木精或其他染料染细胞核不能成功时，用它能得到良好的结果。用番红和结晶紫作染色体的二重染色，染色体染成红色，纺锤丝染成紫色，所以也是一种显示细胞分裂的优良染色剂。用结晶紫染纤毛，效果也很好。用结晶紫染色的切片，缺点是不易长久保存。

AppliChem公司是德国知名的跨国化学产品公司，专注生产常规生化试剂、化学试剂、其产品覆盖了生物化学、分子生物学、细胞生物学、微生物学、免疫学、蛋白组学、植物学、基础化学、制药与诊断试剂研发生产等领域。AppliChem为多家制药、化工等知名企业以及生物技术、生化试剂等著名品牌，如美国Sigma、德国Merck等，提供产品。除了作为OEM供货商，全世界有成千上万的公共科研机构、大学以及生物技术公司通过直接订购，正在使用AppliChem的优质产品和服务。

产品信息

品牌

货号

品名

规格

AppliChem

A0577

Crystal violet – Solution

100ml/500ml

AppliChem的所有产品都通过了全球ISO 9001资格认证，这保证了所有AppliChem的产品都被精心的监控，并能保证为客户提供他们所需的最优品质的产品。AppliChem作为多家著名试剂品牌的OEM供货商，因此其价格非常有竞争力；在供货渠道上的简化，订货时间也相应缩短，并且能根据客户的需求提供多样产品，几乎能满足客户所有的需求。

相关产品：

品牌

货号

品名

规格

AppliChem

A0691

Crystal violet (C.I. 42555)

25g/100g/250g

上海金畔生物科技有限公司公司还会根据客户的实际需求提供不同类型的生化试剂产品，如针对植物生化试剂的美国著名植物品牌PhytoTech的抗生素、植物凝胶等；针对信号转导研究中的拮抗物、激动剂等的BioTrend生化分子等；还有Accurate的多种生化试剂供您选择。如果你对上述产品及相关技术信息感兴趣，请致电021-50837765 到上海金畔生物科技有限公司垂询相关的最优产品和解决方案，或索取相关手册和资料。我们会为您做的更多！

荧光染料Direct Violet 1(Synonyms: 直接青莲N；直接紫1；直接紫N)

发表于由

荧光染料Dye Reagents Direct Violet 1;(Synonyms: 直接青莲N；直接紫1；直接紫N)

Direct Violet 1 是一种偶氮染料，是一种纺织品染料。Direct Violet 1 还是一种 SARS-CoV-2 spike 蛋白与 ACE2 的蛋白-蛋白相互作用 (PPI) 的抑制剂，IC50 为 1.47-2.63 µM。

Direct Violet 1amp;;(Synonyms: 直接青莲N；直接紫1；直接紫N)

Direct Violet 1 Chemical Structure

CAS No. : 2586-60-9

规格 价格 是否有货 数量
10 mg ￥500 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Direct Violet 1 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Antiviral Compound Library
  • Anti-Obesity Compound Library

生物活性

Direct Violet 1, an azo dye, is a textile dye. Direct Violet 1 is also the protein-protein interaction (PPI) between the SARS-CoV-2 spike protein and ACE2 inhibitor with IC50s of 1.47-2.63 µM[1][2].

IC50 Target

IC50: 1.47 µM (SARS-CoV-2-S-RBD binding to ACE2); 2.63 µM (SARS-CoV-S1S2 binding to ACE2); 2.11 µM (TNF-R1-TNFα binding)[1]
体外研究
(In Vitro)

Direct Violet 1 inhibits SARS-CoV-2-S-RBD binding to human ACE2, inhibits SARS-CoV-S1S2 binding to ACE2, and inhibits TNF-R1 binding to TNFα with IC50 values of 1.47 µM, 2.63 µM, and 2.11 µM, respectively[1].
Direct Violet 1 inhibits SARS-CoV-2 pseudovirus entry into hACE2 expressing host cells in a concentration-dependent manner with an IC50 of 35.8 µM[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

728.66

Formula

C32H22N6Na2O8S2
CAS 号

2586-60-9
中文名称

直接青莲N；直接紫1；直接紫N
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

H2O : 62.5 mg/mL (85.77 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.3724 mL 6.8619 mL 13.7238 mL
5 mM 0.2745 mL 1.3724 mL 2.7448 mL
10 mM 0.1372 mL 0.6862 mL 1.3724 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Damir Bojadzic, et al. Small-Molecule In Vitro Inhibitors of the Coronavirus Spike-ACE2 Protein-Protein Interaction as Blockers of Viral Attachment and Entry for SARS-CoV-2. bioRxiv, 2020 Oct 22.

    [2]. Wafaa M Abd El-Rahim, et al. Genotoxicity studies on the removal of a direct textile dye by a fungal strain, in vivo, using micronucleus and RAPD-PCR techniques on male rats. J Appl Toxicol. 2008 May;28(4):484-90.

Crystal Violet(Synonyms: 结晶紫 Basic Violet 3 Gentian Violet Methyl Violet 10B)

发表于由

Crystal Violet;(Synonyms: 结晶紫; Basic Violet 3; Gentian Violet; Methyl Violet 10B) 纯度: 95.54%

Crystal violet (Basic Violet 3) 是革兰氏染色染料。Crystal Violet (Gentian Violet) 对 H1N1 具有抗病毒作用，并且具有显着的杀菌活性。

Crystal Violetamp;;(Synonyms: 结晶紫; Basic Violet 3; Gentian Violet; Methyl Violet 10B)

Crystal Violet Chemical Structure

CAS No. : 548-62-9

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥550 In-stock
500 mg ￥350 In-stock
5 g ￥450 In-stock
10 g ￥650 In-stock
50 g ; 询价 ;

* Please select Quantity before adding items.

Crystal Violet 相关产品

bull;相关化合物库:

  • Antibacterial Compound Library
  • FDA Approved amp; Pharmacopeial Drug Library
  • Drug Repurposing Compound Library Plus
  • Clinical Compound Library Plus
  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Clinical Compound Library
  • Antiviral Compound Library
  • Drug Repurposing Compound Library

生物活性

Crystal violet (Basic Violet 3) is a triarylmethane dye. Crystal Violet (Gentian Violet) has antiviral effects against H1N1 and also has prominent bactericidal activities.

体外研究
(In Vitro)

Crystal violet (Basic Violet 3) is a component of Gram staining that allows one to recognize the difference between gram-positive and gram-negative bacteria with differential staining. The compound helps to access bacterial contamination of tissue culture samples. The staining response is due to the difference in the chemical and structural composition of the cell walls in different bacteria. Crystal Violet can also be used to test the different species of archaea and the cytostatic/cytotoxic effects on tumor cell lines [1][2][3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Clinical Trial

分子量

407.98

Formula

C25H30ClN3
CAS 号

548-62-9
中文名称

结晶紫；龙胆紫；甲基紫
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture and light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture and light)
溶解性数据
In Vitro:;

DMSO : ≥ 100 mg/mL (245.11 mM)

H2O : 5 mg/mL (12.26 mM; Need ultrasonic)

* “≥” means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4511 mL 12.2555 mL 24.5110 mL
5 mM 0.4902 mL 2.4511 mL 4.9022 mL
10 mM 0.2451 mL 1.2256 mL 2.4511 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Beveridge, T.J. and S. Schultze-Lam, The response of selected members of the archaea to the gram stain. Microbiology, 1996. 142 ( Pt 10): p. 2887-95.

    [2]. Coico, R., Gram staining. Curr Protoc Microbiol, 2005. Appendix 3: p. Appendix 3C.

    [3]. Bil, J., et al., Statins potentiate cytostatic/cytotoxic activity of sorafenib but not sunitinib against tumor cell lines in vitro. Cancer Lett, 2010. 288(1): p. 57-67.

    [4]. Nagayama A. Inactivation of influenza A virus by gentian violet (GV) and GV-dyed cotton cloth, and bactericidal activities of these agents. J Infect Chemother. 2006 Apr;12(2):73-9.

荧光染料Cresyl Violet perchlorate(Synonyms: Oxazine 9 perchlorate)

发表于由

荧光染料Dye Reagents Cresyl Violet perchlorate;(Synonyms: Oxazine 9 perchlorate)

Cresyl violet perchlorate 是一种红色荧光染色剂，可用于染色神经元。

Cresyl Violet perchlorateamp;;(Synonyms: Oxazine 9 perchlorate)

Cresyl Violet perchlorate Chemical Structure

CAS No. : 41830-80-2

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Cresyl Violet perchlorate 的其他形式现货产品:

Cresyl Violet acetate

生物活性

Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.

体外研究
(In Vitro)

The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet perchlorate (CV) stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet perchlorate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet perchlorate stained and PV immunostained sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in CV stained and PV immunostained sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in CV stained and PV immunostained sections, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

361.74

Formula

C16H12ClN3O5
CAS 号

41830-80-2
中文名称

甲酚紫高氯酸盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.
Cell Assay
[1]

Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl Violet perchlorate (CV) and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl Violet perchlorate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl Violet perchlorate stained sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.

Leucocrystal violet(Synonyms: 无色结晶紫)

发表于由

Leucocrystal violet;(Synonyms: 无色结晶紫) 纯度: 98.39%

Leucocrystal violet 是一种三苯基甲烷染料，可以用分光光度技术检测环境和生物样品中的锑。

Leucocrystal violetamp;;(Synonyms: 无色结晶紫)

Leucocrystal violet Chemical Structure

CAS No. : 603-48-5

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥550 In-stock
500 mg ￥500 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

Leucocrystal violet 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Leucocrystal violet is a triphenylmethane dye which can be used to detect antimony in environmental and biological samples using spectrophotometric techniques.

分子量

373.53

Formula

C25H31N3
CAS 号

603-48-5
中文名称

隐性结晶紫；无色结晶紫
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
溶解性数据
In Vitro:;

DMSO : 12.5 mg/mL (33.46 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.6772 mL 13.3858 mL 26.7716 mL
5 mM 0.5354 mL 2.6772 mL 5.3543 mL
10 mM 0.2677 mL 1.3386 mL 2.6772 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 1.25 mg/mL (3.35 mM); Suspended solution; Need ultrasonic

    此方案可获得 1.25 mg/mL (3.35 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例，取 100 μL 12.5 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Wu J, et al. Biodegradation of leuco derivatives of triphenylmethane dyes by Sphingomonas sp. CM9. Biodegradation. 2011 Sep;22(5):897-904.

    [2]. Tiwari KK, et al. A simple and sensitive analytical method for the determination of antimony in environmental and biological samples. Anal Sci. 2006 Feb;22(2):259-62.

Cresyl Violet acetate(Synonyms: 甲酚紫醋酸盐)

发表于由

Cresyl Violet acetate;(Synonyms: 甲酚紫醋酸盐) 纯度: ge;70.0%

Cresyl Violet acetate 是一种红色荧光染色剂，可用于染色神经元。

Cresyl Violet acetateamp;;(Synonyms: 甲酚紫醋酸盐)

Cresyl Violet acetate Chemical Structure

CAS No. : 10510-54-0

规格 价格 是否有货 数量
100 mg ￥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Cresyl Violet acetate 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Cresyl Violet acetate is a red fluorescent stain, which can be used to stain neurons.

体外研究
(In Vitro)

The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

321.33

Formula

C18H15N3O3
CAS 号

10510-54-0
中文名称

甲酚紫醋酸盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 1 mg/mL (3.11 mM; Need ultrasonic)

H2O : 0.67 mg/mL (2.09 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.1121 mL 15.5603 mL 31.1207 mL
5 mM — — —
10 mM — — —

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.
Cell Assay
[1]

Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl violet acetate and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl violet acetate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl violet acetate staining sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Kaur C, et al. Comparison of unbiased stereological estimation of total number of cresyl violet stained neurons and parvalbumin positive neurons in the adult human spiral ganglion. J Chem Neuroanat. 2017 Jun 23. pii: S0891-0618(17)30037-6.

固紫B盐 Fast Violet B Salt

发表于由

固紫B盐
Fast Violet B Salt

  • 产品特性
  • 相关资料
  • Q&A
  • 参考文献

固紫B盐固紫B盐 Fast Violet B Salt

Fast Violet B Salt

 


　　固紫B盐是一种棕黄色偶氮染料。

　　常用于细胞化学染色法，主要应用于偶氮偶联法中性粒细胞碱性磷酸酶染色。

 


◆产品信息


分子式： C30H28Cl4N6O4Zn
分子量： 743.78
cas号： 14726-28-4
外观： 浅黄棕色至黄棕色结晶或粉末
可溶性： 易溶于水

◆应用


可作为染料，对细胞进行染色，可用于：

●　细胞中的碱性磷酸酶染色

●　骨髓基质细胞的染色

 


◆应用案列


点击此处进一步了解应用案例


※ 本页面产品仅供研究用，研究以外不可使用。


产品列表
产品编号 产品名称 产品规格 产品等级 备注
060-01081 Fast Violet B Salt
固紫B盐		 1.5 g Wako Special Grade –