标签归档：tag

3x DYKDDDDK Tag

发表于由

3x DYKDDDDK Tag;

3x DYKDDDDK Tag 是研究抗体 (Abs) 不可用的蛋白质的功能和定位的有用工具。通常，它也以 3X 标志形式 (3x DYKDDDDK Tag TFA) 用于纯化低丰度蛋白。

3x DYKDDDDK Tagamp;;

3x DYKDDDDK Tag Chemical Structure

CAS No. : 921765-76-6

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

3x DYKDDDDK Tag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance [1][2].

体外研究
(In Vitro)

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3002.88

Formula

D15K6Y3
CAS 号

921765-76-6
Sequence Shortening

DYKDDDDKDYKDDDDKDYKDDDDK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830.

    [2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.

3x DYKDDDDK Tag TFA

发表于由

3x DYKDDDDK Tag TFA;

DYKDDDDK peptide (FLAG) 是研究抗体 (Abs) 不可用的蛋白质的功能和定位的有用工具。通常，它也以 3X 标志形式 (3x DYKDDDDK Tag TFA) 用于纯化低丰度蛋白。

3x DYKDDDDK Tag TFAamp;;

3x DYKDDDDK Tag TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

DYKDDDDK peptide (FLAG) is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag TFA) for purifying difficult proteins that accumulate in low abundance [1][2].

体外研究
(In Vitro)

HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

3116.90

Formula

D15K6Y3
Sequence Shortening

DYKDDDDKDYKDDDDKDYKDDDDK
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020;21(8):821-830.

    [2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017;56(28):3619-3631.

X-press Tag Peptide(Synonyms: X-press标签肽)

发表于由

X-press Tag Peptide;(Synonyms: X-press标签肽)

X-press Tag Peptide 是一种N-端前导肽，Anti-Xpress 抗体能够识别 Xpress 表位，因此，该多肽可用来纯化 X-press Tag 融合蛋白。

X-press Tag Peptideamp;;(Synonyms: X-press标签肽)

X-press Tag Peptide Chemical Structure

规格 价格 是否有货
1 mg ￥500 询问价格 货期
5 mg ￥1100 询问价格 货期
10 mg ￥1900 询问价格 货期

* Please select Quantity before adding items.

生物活性

X-press Tag Peptide is a tag peptide used for protein purification. X-press Tag is also an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. Anti-Xpress antibodies recognize the Xpress epitope sequence found in this leader peptide.

分子量

997.96

Formula

C41H59N9O20
Sequence

Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys
Sequence Shortening

DLYDDDDK
中文名称

X-press标签肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

S Tag Peptide(Synonyms: S标签肽)

发表于由

S Tag Peptide;(Synonyms: S标签肽)

S Tag Peptide 是一种合成的多肽，由15个氨基酸残基构成。

S Tag Peptideamp;;(Synonyms: S标签肽)

S Tag Peptide Chemical Structure

规格 价格 是否有货
1 mg ￥500 询问价格 货期
5 mg ￥1100 询问价格 货期
10 mg ￥1900 询问价格 货期

* Please select Quantity before adding items.

生物活性

S Tag Peptide is a 15 amino acid peptide derived from RNase A.

体外研究
(In Vitro)

S Tag sequence is a novel fusion peptide tag for recombinant proteins that allows detection by a rapid, sensitive homogeneous assay or by colorimetric detection in Western blots. S Tag can be applied to purification of target proteins[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1748.91

Formula

C73H117N23O25S
Sequence

Lys-Glu-Thr-Ala-Ala-Ala-Lys-Phe-Glu-Arg-Gln-His-Met-Asp-Ser
Sequence Shortening

KETAAAKFERQHMDS
中文名称

S标签肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Mark McCormick, et al. S Tag: A Multipurpose Fusion Peptide for Recombinant Proteins.

T7 Tag Peptide(Synonyms: T7标签肽)

发表于由

T7 Tag Peptide;(Synonyms: T7标签肽)

T7 Tag Peptide 是一个蛋白标签，由 T7 噬菌体衣壳蛋白 gp 10 N-端的 11个残基组成。T7 Tag Peptide 可用于不同的免疫检测以及亲和纯化。

T7 Tag Peptideamp;;(Synonyms: T7标签肽)

T7 Tag Peptide Chemical Structure

CAS No. : 245445-88-9

规格 价格 是否有货
1 mg ￥600 询问价格 货期
5 mg ￥1200 询问价格 货期
10 mg ￥2100 询问价格 货期

* Please select Quantity before adding items.

T7 Tag Peptide 的其他形式现货产品:

T7 Tag Peptide TFA

生物活性

T7 Tag Peptide is a protein tag derived from the N-terminal 11 residues of the major T7 capsid protein, gp 10. T7 Tag Peptide can be used in different immunoassays as well as affinity purification[1].

分子量

1098.27

Formula

C41H71N13O16S3
CAS 号

245445-88-9
Sequence

Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly
Sequence Shortening

MASMTGGQQMG
中文名称

T7标签肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Tsutomu Tanaka, et al. A Peptidyl Linker for Protein Cross-Linking Catalyzed by Microbial Transglutaminase. The Society of Chemical Engineers, Japan. Session ID : 3J-11.

T7 Tag Peptide TFA(Synonyms: T7标签肽三氟乙酸盐)

发表于由

T7 Tag Peptide TFA;(Synonyms: T7标签肽三氟乙酸盐) 纯度: 99.02%

T7 Tag Peptide TFA 是一个蛋白标签，由 T7 噬菌体衣壳蛋白 gp 10 N-端的 11个残基组成。T7 Tag Peptide TFA 可用于不同的免疫检测以及亲和纯化。

T7 Tag Peptide TFAamp;;(Synonyms: T7标签肽三氟乙酸盐)

T7 Tag Peptide TFA Chemical Structure

规格 价格 是否有货 数量
1 mg ￥800 In-stock
5 mg ; 询价 ;
10 mg ; 询价 ;

* Please select Quantity before adding items.

T7 Tag Peptide TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

T7 Tag Peptide TFA is a protein tag derived from the N-terminal 11 residues of the major T7 capsid protein, gp 10. T7 Tag Peptide TFA can be used in different immunoassays as well as affinity purification[1].

分子量

1212.29

Formula

C43H72F3N13O18S3
Sequence Shortening

MASMTGGQQMG
中文名称

T7标签肽三氟乙酸盐
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (41.24 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.8249 mL 4.1244 mL 8.2489 mL
5 mM 0.1650 mL 0.8249 mL 1.6498 mL
10 mM 0.0825 mL 0.4124 mL 0.8249 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (2.06 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.06 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (2.06 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.06 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.06 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.06 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Tsutomu Tanaka, et al. A Peptidyl Linker for Protein Cross-Linking Catalyzed by Microbial Transglutaminase. The Society of Chemical Engineers, Japan. Session ID : 3J-11.

Rhodopsin Epitope Tag

发表于由

Rhodopsin Epitope Tag; 纯度: 99.97%

Rhodopsin Epitope Tag是位于牛视紫红质的C端区域内的9个氨基酸的肽。Rhodopsin Epitope Tag被广泛用作表位标签，并可被许多抗视紫红质抗体识别。

Rhodopsin Epitope Tagamp;;

Rhodopsin Epitope Tag Chemical Structure

规格 价格 是否有货 数量
1 mg ￥900 In-stock
5 mg ￥2700 In-stock
10 mg ￥4400 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

* Please select Quantity before adding items.

Rhodopsin Epitope Tag 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Rhodopsin Epitope Tag is a 9-amino acid peptide localized within the C-terminal region of bovine rhodopsin . Rhodopsin Epitope Tag is widely used as an epitope tag and can be recognized by a number of anti-rhodopsin antibodies.

体外研究
(In Vitro)

Rhodopsin Epitope Tag is a 9-amino acid peptide localized within the C-terminal region of bovine rhodopsin . Rhodopsin Epitope Tag is widely used as an epitope tag and can be recognized by a number of anti-rhodopsin antibodies. The specificities of four monoclonal antibodies rho 1D4, 1C5, 3A6, and 3D6 prepared by immunization of rod outer segments containing rhodopsin have been defined using synthetic peptides. All of these antibodies interact within the 18 residues at the COOH terminus of rhodopsin and recognize linear antigenic determinants of 4-11 residues[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

902.95

Formula

C37H62N10O16
Sequence

Thr-Glu-Thr-Ser-Gln-Val-Ala-Pro-Ala
Sequence Shortening

TETSQVAPA
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Hodges RS, Heaton RJ, Parker JM, Molday L, Molday RS. Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin. J Biol Chem. 1988;263:11768–11775.