标签归档：press

X-press Tag Peptide(Synonyms: X-press标签肽)

发表于由

X-press Tag Peptide;(Synonyms: X-press标签肽)

X-press Tag Peptide 是一种N-端前导肽，Anti-Xpress 抗体能够识别 Xpress 表位，因此，该多肽可用来纯化 X-press Tag 融合蛋白。

X-press Tag Peptideamp;;(Synonyms: X-press标签肽)

X-press Tag Peptide Chemical Structure

规格 价格 是否有货
1 mg ￥500 询问价格 货期
5 mg ￥1100 询问价格 货期
10 mg ￥1900 询问价格 货期

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生物活性

X-press Tag Peptide is a tag peptide used for protein purification. X-press Tag is also an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. Anti-Xpress antibodies recognize the Xpress epitope sequence found in this leader peptide.

分子量

997.96

Formula

C41H59N9O20
Sequence

Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys
Sequence Shortening

DLYDDDDK
中文名称

X-press标签肽
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.