标签归档：p

p5 Ligand for Dnak and DnaJ

发表于由

p5 Ligand for Dnak and DnaJ;

p5 Ligand for Dnak and DnaJ 是一种九肽，对应于线粒体天冬氨酸氨基转移酶前序列的 23 个残基部分的主要结合位点。p5 Ligand for Dnak and DnaJ 是 DnaK (E. coli Hsp70) 和 DnaJ (E. coli Hsp40) 的高亲和力配体。

p5 Ligand for Dnak and DnaJamp;;

p5 Ligand for Dnak and DnaJ Chemical Structure

CAS No. : 209518-24-1

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生物活性

p5 Ligand for Dnak and DnaJ is a nonapeptide, which corresponds to the main binding site for the 23-residue part of the presequence of mitochondrial aspartate aminotransferase. p5 Ligand for Dnak and DnaJ is a high-affinity ligand for DnaK and DnaJ[1].

分子量

1028.27

Formula

C44H81N15O11S
CAS 号

209518-24-1
Sequence

Cys-Leu-Leu-Leu-Ser-Ala-Pro-Arg-Arg
Sequence Shortening

CLLLSAPRR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Swain JF, et al. Hsp70 chaperone ligands control domain association via an allosteric mechanism mediated by the interdomain linker. Mol Cell. 2007 Apr 13;26(1):27-39.

Gliadin p31-43

发表于由

Gliadin p31-43;

Gliadin p31-43 是一种未消化的麦醇溶蛋白肽，可诱导肠道内的先天性免疫反应并干扰内吞运输。Gliadin p31-43 可用于乳糜泻的研究。

Gliadin p31-43amp;;

Gliadin p31-43 Chemical Structure

CAS No. : 176326-01-5

规格 价格 是否有货 数量
5 mg ￥5500 In-stock
10 mg ￥8800 In-stock
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Gliadin p31-43 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Gliadin p31-43 is an undigested gliadin peptide. Gliadin p31-43 induces an innate immune response in the intestine and interferes with endocytic trafficking. Gliadin p31-43 can be used for celiac disease research[1][2].

体外研究
(In Vitro)

Gliadin p31-43 (100 μg/mL; 30 minutes-6 hours) treatment induces the MyD88/TLR7 complexes, and activates downstream signalling by activating MAPKs, ERK, JNK and p38). Gliadin p31-43 increases the levels of the phosphorylated forms of pY-ERK, JNK (pY-JNK) and p38 (pY-p38)[1].
Gliadin p31-43 treatment increases NF-κB phosphorylation in CaCo-2 cells from 0.45 in control cells to 0.86. Gliadin p31-43 treatment induces a significant increase in levels of the MxA protein. The levels of the IFN-α 7 and 17 mRNAs are also analysed after Gliadin p31-43 treatment[1].
In CaCo-2 cells, Gliadin p31-43 localizes to the early endosomes and delays vesicular trafficking. Gliadin p31-43 interferes with the correct localization of the growth factor regulated tyrosine kinase substrate (HRS) to early endosomes, delaying the maturation of the endocytic vesicles[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: CaCo-2 cells
Concentration: 100 μg/mL
Incubation Time: 30 minutes, 3 hours, 6 hours
Result: Showed the increase in formation of the MyD88/TLR7 complex, and increased in the level of TLR7.

体内研究
(In Vivo)

Gliadin p31-43 (10 μg; intraluminally injection) shows a sequence-specific spontaneous ability to form structured oligomers and aggregates in vitro and induced activation of the apoptosis-associated speck-like (ASC) complex[2].
The increment of IL-1β indicates the activation of the inflammasome caspase-1 pathway in the small intestine mucosa by oral administration of Gliadin p31-43 (20 μg) in wild type C57Bl/6 mice. Gliadin p31-43 has an intrinsic propensity to form oligomers which trigger the NLRP3 inflammasome[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1527.68

Formula

C71H102N18O20
CAS 号

176326-01-5
Sequence Shortening

LGQQQPFPPQQPY
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 50 mg/mL (32.73 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.6546 mL 3.2729 mL 6.5459 mL
5 mM 0.1309 mL 0.6546 mL 1.3092 mL
10 mM 0.0655 mL 0.3273 mL 0.6546 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Merlin Nanayakkara, et al. P31-43, an undigested gliadin peptide, mimics and enhances the innate immune response to viruses and interferes with endocytic trafficking: a role in celiac disease. Sci Rep. 2018 Jul 17;8(1):10821.

    [2]. María Florencia Gómez Castro, et al. p31-43 Gliadin Peptide Forms Oligomers and Induces NLRP3 Inflammasome/Caspase 1- Dependent Mucosal Damage in Small Intestine. Front Immunol. 2019 Jan 30;10:31.

PTD-p65-P1 Peptide

发表于由

PTD-p65-P1 Peptide;

PTD-p65-P1 Peptide 是一种核转录因子 NF-kappaB 抑制剂，由一个 antennapedia 衍生的膜转运肽序列 (PTD) 连接 p65-P1 构成, 选择性地抑制由各种刺激诱导的 NF-kappaB 活性。

PTD-p65-P1 Peptideamp;;

PTD-p65-P1 Peptide Chemical Structure

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PTD-p65-P1 Peptide 的其他形式现货产品:

PTD-p65-P1 Peptide TFA

生物活性

PTD-p65-P1 Peptide is a nuclear transcription factor NF-kappaB inhibitor, composed of a membrane-translocating peptide sequence generated from antennapedia (PTD) conjugated with p65-P1, which selectively inhibits activation induced by various inflammatory stimuli[1].

IC50 Target

NF-kappaB[1]
分子量

3829.50

Formula

C168H275N57O44S
Sequence Shortening

DRQIKIWFQNRRMKWKKQLRRPSDRELSE
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Takada Y, et al. Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis. J Biol Chem. 2004 Apr 9;279(15):15096-104.

TAT-P4-(DATC5)2

发表于由

TAT-P4-(DATC5)2;

TAT-P4-(DATC5)2 是一种蛋白激酶 C 相互作用蛋白 1 PDZ 结构域 (PICK1 PDZ domain) 的高亲和力多肽抑制剂，其 Ki 值为 1.7 nM。TAT-P4-(DATC5)2 能抑制大鼠可卡因成瘾。

TAT-P4-(DATC5)2amp;;

TAT-P4-(DATC5)2 Chemical Structure

规格 是否有货
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生物活性

TAT-P4-(DATC5)2 is a high-affinity peptide inhibitor of the PICK1 (protein interacting with C kinase-1) PDZ domain, with a Ki of 1.7 nM. TAT-P4-(DATC5)2 attenuats the reinstatement of cocaine seeking in rats[1].

IC50 Target

Ki: 1.7 nM (PICK1 PDZ domain)[1]
体内研究
(In Vivo)

TAT-P4-(DATC5)2 (0.99 μg/kg, 9.9 μg/kg; Infused intravenously) dose-dependently attenuats the reinstatement of cocaine seeking in rats at doses that do not produce operant learning deficits or suppress locomotor activity [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (250-300 g) [1]
Dosage: 0.99 μg/kg, 9.9 μg/kg
Administration: Infused intravenously, 45 min prior to an acute injection of cocaine (10 mg/kg, i.p.)
Result: Attenuated the reinstatement of cocaine seeking in rats.

分子量

3289.95

Formula

C14H24O6
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Christopher Turner, et al. Administration of a Novel High Affinity PICK1 PDZ Domain Inhibitor Attenuates Cocaine Seeking in Rats. Neuropharmacology. 2020 Mar 1;164:107901.

Cecropin P1, porcine

发表于由

Cecropin P1, porcine;

Cecropin P1, porcine is an antibacterial peptide originally identified in moths (Hyalophora cecropia) and later in pig intestine.

Cecropin P1, porcineamp;;

Cecropin P1, porcine Chemical Structure

CAS No. : 125667-96-1

规格 价格 是否有货
1 mg ￥2900 询问价格 货期
5 mg ￥6500 询问价格 货期

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生物活性

Cecropin P1, porcine is an antibacterial peptide originally identified in moths (Hyalophora cecropia) and later in pig intestine.

分子量

3338.86

Formula

C147H253N45O43
CAS 号

125667-96-1
Sequence

Ser-Trp-Leu-Ser-Lys-Thr-Ala-Lys-Lys-Leu-Glu-Asn-Ser-Ala-Lys-Lys-Arg-Ile-Ser-Glu-Gly-Ile-Ala-Ile-Ala-Ile-Gln-Gly-Gly-Pro-Arg
Sequence Shortening

SWLSKTAKKLENSAKKRISEGIAIAIQGGPR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

HIV p17 Gag (77-85)

发表于由

HIV p17 Gag (77-85);

HIV p17 Gag (77-85) 是一种 HLA-A*0201(A2)-限制性 CTL 表位，用于抗 HIV 的研究。

HIV p17 Gag (77-85)amp;;

HIV p17 Gag (77-85) Chemical Structure

CAS No. : 147468-65-3

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生物活性

HIV p17 Gag (77-85) is an HLA-A*0201(A2)-restricted CTL epitope, used in the research of anti-HIV[1].

分子量

981.10

Formula

C44H72N10O15
CAS 号

147468-65-3
Sequence

Ser-Leu-Tyr-Asn-Thr-Val-Ala-Thr-Leu
Sequence Shortening

SLYNTVATL
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Kan-Mitchell J, et al. Degeneracy and repertoire of the human HIV-1 Gag p17(77-85) CTL response. J Immunol. 2006 Jun 1;176(11):6690-701.

LyP-1

发表于由

LyP-1;

LyP-1 是一种环状 9 个氨基酸的肿瘤的定位肽，在多种肿瘤相关细胞中，选择性结合其 p32 receptor 蛋白。

LyP-1amp;;

LyP-1 Chemical Structure

CAS No. : 454487-07-1

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LyP-1 的其他形式现货产品:

LyP-1 TFA

生物活性

LyP-1 is a cyclic 9‐amino‐acids tumor homing peptide and selectively bind to p32 receptors overexpressed in various tumor-associated cells[1].

体内研究
(In Vivo)

LyP-1 (8 weeks) shows a remarkable reduction in plaque formation and plaque occupation rates in the LyP-1-treated group. In addition, a higher apoptotic rate in macrophages released from hypoxic plaques is observed after the treatment of LyP-1when compared to control peptide[1].
The LyP-1 peptide is labeled with a near-infrared fluorophore (Cy5.5) for optical imaging.
At days 3, 7, 14 and 21 after inoculation with 4T1 cells, tumor-bearing BALB/C mice is injected Cy5.5-LyP-1 (0.8 nmol) through the middle phalanges of the upper extremities of the tumor-bearing mice. The fluorescence intensities were 0.024, 0.038, 0.048 and 0.106×106 photon/cm2/sec respectively at days 3, 7, 14 and 21 after tumor cell inoculation, which are 1.02, 1.63, 2.04, and 4.52-fold higher than in the contralateral LNs. Cy5.5-LyP-1 staining in LNs co-localized with LYVE-1, suggesting lymphatics-specific binding of LyP-1 peptide[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

992.14

Formula

C36H65N17O12S2
CAS 号

454487-07-1
Sequence Shortening

CGNKRTRGC (Disulfide bridge: Cys1-Cys9)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Fan Zhang, et al.Imaging tumor-induced sentinel lymph node lymphangiogenesis with LyP-1 peptide. Amino Acids. 2012 Jun;42(6):2343-51.

    [2]. Ningning Song, et al. Recent progress in LyP-1-based strategies for targeted imaging and therapy. Drug Deliv. 2019 Dec;26(1):363-375.

TAT-P4-(DATC5)2 TFA

发表于由

TAT-P4-(DATC5)2 TFA;

TAT-P4-(DATC5)2 TFA 是一种蛋白激酶 C 相互作用蛋白 1 PDZ 结构域 (PICK1 PDZ domain) 的高亲和力多肽抑制剂，其 Ki 值为 1.7 nM。TAT-P4-(DATC5)2 TFA 能抑制大鼠可卡因上瘾。

TAT-P4-(DATC5)2 TFAamp;;

TAT-P4-(DATC5)2 TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

TAT-P4-(DATC5)2 TFA is a high-affinity peptide inhibitor of the PICK1 (protein interacting with C kinase-1) PDZ domain, with a Ki of 1.7 nM. TAT-P4-(DATC5)2 TFA attenuats the reinstatement of cocaine seeking in rats[1].

IC50 Target

Ki: 1.7 nM (PICK1 PDZ domain)[1]
体内研究
(In Vivo)

TAT-P4-(DATC5)2 TFA (0.99 μg/kg, 9.9 μg/kg; Infused intravenously) dose-dependently attenuats the reinstatement of cocaine seeking in rats at doses that do not produce operant learning deficits or suppress locomotor activity [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (250-300 g) [1]
Dosage: 0.99 μg/kg, 9.9 μg/kg
Administration: Infused intravenously, 45 min prior to an acute injection of cocaine (10 mg/kg, i.p.)
Result: Attenuated the reinstatement of cocaine seeking in rats.

分子量

3403.97

Formula

C16H25F3O8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Christopher Turner, et al. Administration of a Novel High Affinity PICK1 PDZ Domain Inhibitor Attenuates Cocaine Seeking in Rats. Neuropharmacology. 2020 Mar 1;164:107901.

Gliadin p31-43 TFA

发表于由

Gliadin p31-43 TFA;

Gliadin p31-43 TFA 是一种未消化的麦醇溶蛋白肽，可诱导肠道内的先天性免疫反应并干扰内吞运输。Gliadin p31-43 TFA 可用于乳糜泻的研究。

Gliadin p31-43 TFAamp;;

Gliadin p31-43 TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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Gliadin p31-43 TFA 的其他形式现货产品:

Gliadin p31-43

生物活性

Gliadin p31-43 TFA is an undigested gliadin peptide. Gliadin p31-43 TFA induces an innate immune response in the intestine and interferes with endocytic trafficking. Gliadin p31-43 TFA can be used for celiac disease research[1][2].

体外研究
(In Vitro)

Gliadin p31-43 (100 μg/mL; 30 minutes-6 hours) treatment induces the MyD88/TLR7 complexes, and activates downstream signalling by activating MAPKs, ERK, JNK and p38). Gliadin p31-43 increases the levels of the phosphorylated forms of pY-ERK, JNK (pY-JNK) and p38 (pY-p38)[1].
Gliadin p31-43 treatment increases NF-κB phosphorylation in CaCo-2 cells from 0.45 in control cells to 0.86. Gliadin p31-43 treatment induces a significant increase in levels of the MxA protein. The levels of the IFN-α 7 and 17 mRNAs are also analysed after Gliadin p31-43 treatment[1].
In CaCo-2 cells, Gliadin p31-43 localizes to the early endosomes and delays vesicular trafficking. Gliadin p31-43 interferes with the correct localization of the growth factor regulated tyrosine kinase substrate (HRS) to early endosomes, delaying the maturation of the endocytic vesicles[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[1]

Cell Line: CaCo-2 cells
Concentration: 100 μg/mL
Incubation Time: 30 minutes, 3 hours, 6 hours
Result: Showed the increase in formation of the MyD88/TLR7 complex, and increased in the level of TLR7.

体内研究
(In Vivo)

Gliadin p31-43 (10 μg; intraluminally injection) shows a sequence-specific spontaneous ability to form structured oligomers and aggregates in vitro and induced activation of the apoptosis-associated speck-like (ASC) complex[2].
The increment of IL-1β indicates the activation of the inflammasome caspase-1 pathway in the small intestine mucosa by oral administration of Gliadin p31-43 (20 μg) in wild type C57Bl/6 mice. Gliadin p31-43 has an intrinsic propensity to form oligomers which trigger the NLRP3 inflammasome[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1641.70

Formula

C73H103F3N18O22
Sequence Shortening

LGQQQPFPPQQPY
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Merlin Nanayakkara, et al. P31-43, an undigested gliadin peptide, mimics and enhances the innate immune response to viruses and interferes with endocytic trafficking: a role in celiac disease. Sci Rep. 2018 Jul 17;8(1):10821.

    [2]. María Florencia Gómez Castro, et al. p31-43 Gliadin Peptide Forms Oligomers and Induces NLRP3 Inflammasome/Caspase 1- Dependent Mucosal Damage in Small Intestine. Front Immunol. 2019 Jan 30;10:31.

Peripheral Myelin Protein P2 (53-78), bovine

发表于由

Peripheral Myelin Protein P2 (53-78), bovine;

Peripheral Myelin Protein P2 (53-78), bovine 是源自牛外周髓磷脂 P2 蛋白氨基酸残基 53-78。Peripheral Myelin Protein P2 (53-78), bovine 是一种 T 细胞抗原表位，可在 Lewis 大鼠中诱导实验性自身免疫性神经炎 (EAN)。

Peripheral Myelin Protein P2 (53-78), bovineamp;;

Peripheral Myelin Protein P2 (53-78), bovine Chemical Structure

CAS No. : 81628-50-4

规格 是否有货
100 mg ; 询价 ;
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生物活性

Peripheral Myelin Protein P2 (53-78), bovine is derived from bovine peripheral myelin P2 protein amino acid residues 53-78. Peripheral Myelin Protein P2 (53-78), bovine is a T cell epitope for the induction of experimental autoimmune neuritis (EAN) in Lewis rats[1].

分子量

3019.19

Formula

C131H200N34O48
CAS 号

81628-50-4
Sequence

Thr-Glu-Ser-Pro-Phe-Lys-Asn-Thr-Glu-Ile-Ser-Phe-Lys-Leu-Gly-Gln-Glu-Phe-Glu-Glu-Thr-Thr-Ala-Asp-Asn-Arg
Sequence Shortening

TESPFKNTEISFKLGQEFEETTADNR
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. A Rostami, et al. Peptide 53-78 of myelin P2 protein is a T cell epitope for the induction of experimental autoimmune neuritis. Cell Immunol. 1991 Feb;132(2):433-41.