Glucagon (1-29), bovine, human, porcine hydrochloride;(Synonyms: 盐酸胰高血糖素; Porcine glucagon hydrochloride)
Glucagon (1-29), bovine, human, porcine hydrochloride 是由胰腺 α 细胞产生的一种多肽激素。Glucagon (1-29), bovine, human, porcine hydrochloride 促进糖异生。Glucagon (1-29), bovine, human, porcine hydrochloride 可激活 HNF4α 并促进 HNF4α 磷酸化。
Glucagon (1-29), bovine, human, porcine hydrochloride Chemical Structure
CAS No. : 28270-04-4
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| 生物活性 |
Glucagon (1-29), bovine, human, porcine hydrochloride is a peptide hormone, produced by pancreatic α-cells. Glucagon hydrochloride stimulates gluconeogenesis[1]. Glucagon (1-29), bovine, human, porcine hydrochloride activates HNF4α and increases HNF4α phosphorylation[2][3].
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体外研究
(In Vitro) |
Upon binding to its receptor Gcgr, Glucagon activates cAMP-PKA signaling to stimulate hepatic glucose production (HGP) and cause hyperglycemia[1].
Glucagon stimulates both hepatic kisspeptin1 production and gluconeogenesis[1].
Glucagon (100 nM) represses CYP7A1 mRNA expression in human primary hepatocytes[3].
Glucagon (100 nM) increases phosphorylayion of HNF4α[3].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Western Blot Analysis[3]
| Cell Line: |
Human primary hepatocytes (H1211, HH1215) |
| Concentration: |
100 nM |
| Incubation Time: |
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| Result: |
Resulted in a marked increase in the amount of phosphorylated HNF4α. |
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体内研究
(In Vivo) |
Low-dose (20 μg/kg) Glucagon increases glycemia and does not stimulate insulin secretion in ambient-fed mice. High-dose (1 mg/kg) Glucagon lowers glycemia compared with PBS control and stimulates insulin secretion in ambient-fed mice[4].
MCE has not independently confirmed the accuracy of these methods. They are for reference only.
| Animal Model: |
C57BL/6J mice (12- to 24-week-old)[4] |
| Dosage: |
20 μg/kg and 1 mg/kg |
| Administration: |
Administered by i.p. injection; 45 minutes |
| Result: |
Low-dose (20 μg/kg) increased glycemia and did not stimulate insulin secretion.
High-dose (1 mg/kg) lowered glycemia and stimulated insulin secretion. |
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| 分子量 |
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| Formula |
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| CAS 号 |
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| Sequence |
His-Ser-Gln-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Lys-Tyr-Leu-Asp-Ser-Arg-Arg-Ala-Gln-Asp-Phe-Val-Gln-Trp-Leu-Met-Asn-Thr
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| Sequence Shortening |
HSQGTFTSDYSKYLDSRRAQDFVQWLMNT
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| 中文名称 |
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| 运输条件 |
Room temperature in continental US; may vary elsewhere.
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| 储存方式 |
Please store the product under the recommended conditions in the Certificate of Analysis.
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| 参考文献 |
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[1]. Song WJ, et al. Glucagon regulates hepatic kisspeptin to impair insulin secretion. Cell Metab. 2014 Apr 1;19(4):667-81.
[2]. Hirota K, et al. Hepatocyte nuclear factor-4 is a novel downstream target of insulin via FKHR as a signal-regulated transcriptional inhibitor. J Biol Chem. 2003 Apr 11;278(15):13056-60.
[3]. Song KH, et al. Glucagon and cAMP inhibit cholesterol 7alpha-hydroxylase (CYP7A1) gene expression in humanhepatocytes: discordant regulation of bile acid synthesis and gluconeogenesis. Hepatology. 2006 Jan;43(1):117-25.
[4]. Capozzi ME, et al. Glucagon lowers glycemia when β-cells are active. JCI Insight. 2019 Jul 23;5. pii: 129954.
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