Lactoferricin B (4-14), bovine TFA

Lactoferricin B (4-14), bovine TFA;

Lactoferricin B (4-14), bovine (TFA) 对应于牛乳铁蛋白的残基 4-14,具有广泛的抗菌活性。

Lactoferricin B (4-14), bovine TFAamp;;

Lactoferricin B (4-14), bovine TFA Chemical Structure

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生物活性

Lactoferricin B (4-14), bovine (TFA), a peptide corresponding to residues 4-14 of bovine lactoferrin, has antimicrobial activity against a wide range of microorganisms[1].

分子量

1658.89

Formula

C72H114F3N25O15S

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. John S Mjøen Svendsen, et al. Very Short and Stable Lactoferricin-Derived Antimicrobial Peptides: Design Principles and Potential Uses. Acc Chem Res. 2019 Mar 19;52(3):749-759.

β-Casomorphin, bovine TFA(Synonyms: β-Casomorphin-7 (bovine) (TFA) Bovine β-casomorphin-7 TFA)

β-Casomorphin, bovine TFA;(Synonyms: β-Casomorphin-7 (bovine) (TFA); Bovine β-casomorphin-7 TFA)

β-casomorphin, bovine TFA (β-Casomorphin-7 (bovine) TFA) 是阿片 (opioid) 活性的多肽,与阿片受体结合的 IC50 值为 14 μM。

β-Casomorphin, bovine TFAamp;;(Synonyms: β-Casomorphin-7 (bovine) (TFA); Bovine β-casomorphin-7 TFA)

β-Casomorphin, bovine TFA Chemical Structure

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5 mg ¥800 询问价格 货期
10 mg ¥1400 询问价格 货期
25 mg ¥2900 询问价格 货期

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beta;-Casomorphin, bovine TFA 的其他形式现货产品:

beta;-Casomorphin, bovine

生物活性

β-Casomorphin, bovine TFA (β-Casomorphin-7 (bovine) TFA) is a opioid peptide with an IC50 of 14 μM in an Opioid receptors binding assay[1].

IC50 Target

IC50: 14 μM (Opioid receptor)[1]

体外研究
(In Vitro)

β-Casomorphin, bovine TFA (0.1μM; 30 minutes-24 hours) stimulates the secretion of mucin in DHE Cells after 8 h of treatment[2].
β-Casomorphin, bovine TFA (0.1μM; 24 hours) induces an increase in rMuc2 and rMuc3 mRNA levels[2].
β-Casomorphin, bovine TFA does not modify the expression of rMuc1, rMuc4, and rMuc5AC[2].
β-Casomorphin, bovine TFA (0.1μM; 24 hours) stimulates MUC5AC expression and mucin Secretion in HT29-MTX Cells (human)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

β-Casomorphin, bovine TFA (7.5×10−6 mol/day/kg ; i.g.; for 30 days) increases plasma insulin and decreased plasma glucagon of diabetic rats[3].
β-Casomorphin, bovine TFA (7.5×10−6 mol/day/kg ; i.g.; for 30 days) alters the changes of SOD, GPx, T-AOC, MDA and H2O2 in the kidney of diabetic rats[3].
β-Casomorphin, bovine TFA (7.5×10−6 mol/day/kg ; i.g.; for 30 days) reduces MDA (significantly) and H2O2 (non-significantly) level in the kidney compared to the diabetic rats[3].
β-Casomorphin, bovine TFA (7.5×10−6 mol/day/kg ; i.g.; for 30 days) alleviates high glucose-induced decreasement in SOD and GPx activity, increasement in MDA contents in the NRK-52E cells[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley (SD) rats (200 g±10 g)[3]
Dosage: 7.5×10−6 mol/day/kg
Administration: Oral gavage; for 30 days
Result: Increased plasma insulin and decreased plasma glucagon of diabetic rats.

分子量

903.94

Formula

C43H56F3N7O11

Sequence

Tyr-Pro-Phe-Pro-Gly-Pro-Ile

Sequence Shortening

YPFPGPI

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Brantl V, et al. Opioid activities of beta-casomorphins. Life Sci. 1981 Apr 27;28(17):1903-9.

    [2]. Zoghbi S, et al. β-casomorphin-7-7 regulates the secretion and expression of gastrointestinal mucins through a μ-opioid pathway. Am J Physiol Gastrointest Liver Physiol. 2006 Jun;290(6):G1105-13.

    [3]. Zhang W, et al. The protective effects of beta-casomorphin-7 against glucose -induced renal oxidative stress in vivo and vitro. PLoS One. 2013 May 3;8(5):e63472.

β-Casomorphin (1-5), amide, bovine

β-Casomorphin (1-5), amide, bovine;

β-Casomorphin (1-5), amide, bovine 是牛 β-Casomorphin 的多肽片段。

β-Casomorphin (1-5), amide, bovineamp;;

β-Casomorphin (1-5), amide, bovine Chemical Structure

CAS No. : 83936-23-6

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生物活性

β-Casomorphin (1-5), amide, bovine is a peptide of bovine β-Casomorphin.

分子量

626.77

Formula

C31H42N6O6S

CAS 号

83936-23-6

Sequence

Tyr-{d-Ala}-Phe-Pro-Met-NH2

Sequence Shortening

Y-{d-Ala}-FPM-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

β-Casomorphin (1-6), bovine

β-Casomorphin (1-6), bovine;

β-Casomorphin (1-6), bovine 是一种类阿片的 β-Casomorphin 生物活性肽片段。

β-Casomorphin (1-6), bovineamp;;

β-Casomorphin (1-6), bovine Chemical Structure

CAS No. : 77434-43-6

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生物活性

β-Casomorphin (1-6), bovine is a opioid-like bioactive peptide of β-Casomorphin.

分子量

676.76

Formula

C35H44N6O8

CAS 号

77434-43-6

Sequence Shortening

YPFPGP

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Chang WH, et al. β-Casomorphin increases fat deposition in broiler chickens by modulating expression of lipid metabolism genes. Animal. 2019 Apr;13(4):777-783.

β-Casomorphin (1-5), bovine

β-Casomorphin (1-5), bovine;

β-Casomorphin (1-5), bovine 是牛 β-Casomorphin 的多肽片段。

β-Casomorphin (1-5), bovineamp;;

β-Casomorphin (1-5), bovine Chemical Structure

CAS No. : 72122-63-5

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生物活性

β-Casomorphin (1-5), bovine is a peptide of bovine β-Casomorphin.

分子量

579.64

Formula

C30H37N5O7

CAS 号

72122-63-5

Sequence

Tyr-Pro-Phe-Pro-Gly

Sequence Shortening

YPFPG

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.

Insulin(cattle)(Synonyms: 牛胰岛素 Insulin from bovine pancreas)

Insulin(cattle);(Synonyms: 牛胰岛素; Insulin from bovine pancreas) 纯度: ge;98.0%

Insulin cattle (Insulin from bovine pancreas) 是在胰腺 β 细胞中产生的两链多肽激素。Insulin cattle 在很多哺乳动物细胞培养体系中用作生长因子。

Insulin(cattle)amp;;(Synonyms: 牛胰岛素; Insulin from bovine pancreas)

Insulin(cattle) Chemical Structure

CAS No. : 11070-73-8

规格 价格 是否有货 数量
10 mg ¥600 In-stock
25 mg ¥1100 In-stock
50 mg ¥1600 In-stock
100 mg ¥2700 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

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Insulin(cattle) 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Insulin cattle (Insulin from bovine pancreas) is a two-chain polypeptide hormone produced in vivo in the pancreatic β cells. Insulin cattle has often been used as growth supplement in culturing cells.

体外研究
(In Vitro)

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates glucose uptake into muscle and fat cells by recruiting membrane glucose transporter Glut-4 to cell surface. Insulin cattle has often been used as growth supplement in culturing cells at the concentration ranging from 1 to 10 μg/mL of medium.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

5733.49

Formula

C254H377N65O75S6

CAS 号

11070-73-8

Sequence

Phe-Val-Asn-Gln-His-Leu-Cys-Gly-Ser-His-Leu-Val-Glu-Ala-Leu-Tyr-Leu-Val-Cys-Gly-Glu-Arg-Gly-Phe-Phe-Tyr-Thr-Pro-Lys-Ala. Gly-Ile-Val-Glu-Gln-Cys-Cys-Ala-Ser-Val-Cys-Ser-Leu-Tyr-Gln-Leu-Glu-Asn-Tyr-Cys-Asn (Disulfide bridge: Cys7-Cys7′, Cys19-Cys20′, Cys6′

Sequence Shortening

FVNQHLCGSHLVEALYLVCGERGFFYTPKA. GIVEQCCASVCSLYQLENYCN (Disulfide bridge: Cys7-Cys7′, Cys19-Cys20′, Cys6′-Cys11′)

中文名称

牛胰岛素

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 11 mg/mL (1.92 mM; ultrasonic and adjust pH to 2 with HCl)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.1744 mL 0.8721 mL 1.7441 mL
5 mM
10 mM

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

参考文献
  • [1]. Yousefi R, et al. Aspirin-mediated acetylation induces structural alteration and aggregation of bovine pancreaticinsulin. J Biomol Struct Dyn. 2016;34(2):362-75.

Bovine Serum Albumin(Synonyms: 白蛋白; Albumin bovine serum; BSA)

生化分析试剂 Biochemical Assay Reagents
Bovine Serum Albumin;(Synonyms: 白蛋白; Albumin bovine serum; BSA) 纯度: ge;98.0%

Bovine Serum Albumin (BSA) 是由三个同源全 α 结构域组成的 583 个氨基酸的蛋白质。BSA 是一种球状蛋白,可用于多种生物化学实验。

Bovine Serum Albumin(Synonyms: 白蛋白; Albumin bovine serum;  BSA)

Bovine Serum Albumin Chemical Structure

CAS No. : 9048-46-8

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
500 mg ¥400 In-stock
1 g ¥500 In-stock
10 g ¥2550 In-stock
50 g ; 询价 ;

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Bovine Serum Albumin 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Bovine Serum Albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. BSA is a globular protein that is used in numerous biochemical applications.

体外研究
(In Vitro)

Bovine serum albumin (BSA) is a 583-residue protein consisting of three homologous all-α domains, organized in a heart-shaped structure. Bovine serum albumin constitutes ca. 60% of all plasma protein and binds and transports a large number of physiological and non-physiological ligands. Bovine serum albumin contains 17 disulfide bonds and one unpaired cysteine (Cys34), which facilitates dimerization and also influences higher-order association, since the rate of aggregation is slowed down if Cys34 is covalently bound to another compound. MTT assay for fibril cytotoxicity shows Bovine Serum Albumin is beneficial for cell growth irrespective of its aggregated state. Lack of cytotoxicity is confirmed by membrane permeabilization assays. In the subsequent 40 h, an increase in the viability of the treated cells of 300-400% is observed, indicating that the cells incubated with Bovine Serum Albumin achieve a higher viability than the untreated cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号

9048-46-8

中文名称

牛血清白蛋白;牛白蛋白;卵蛋白粉;血清蛋白;血清白蛋白;牛血蛋白质;牛血清蛋白;牛血清蛋白;牛血清清蛋白;白蛋白

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

H2O : ≥ 176.66 mg/mL

* “≥” means soluble, but saturation unknown.

参考文献
  • [1]. Holm NK, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38.

Cell Assay
[1]

Samples are prepared in 96-well microplates and all wells without samples are filled with 200 μL PBS to minimize evaporation. To each well is loaded 100 μL SH-SY5Y growth medium with or without 10.000 SH-SY5Y neuroblastoma cells. The assay is started by adding 25 μL buffer, with or without 5 mg/mL sonicated Bovine Serum Albumin previously incubated for 0, 2 or 96 h at 70°C (giving a final BSA concentration of 1 mg/mL). All sample combinations are loaded in triplets, and for each of the six Bovine Serum Albumin combinations a corresponding triplet is loaded without cells, to be used as blank. One triplet is also loaded without both cells and BSA and one only without Bovine Serum Albumin[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Holm NK, et al. Aggregation and fibrillation of bovine serum albumin. Biochim Biophys Acta. 2007 Sep;1774(9):1128-38.

Fetuin, Fetal Bovine Serum

Fetuin, Fetal Bovine Serum; 纯度: ge;99.0%

Fetuin, Fetal Bovine Serum 是从胎牛血清分离的肝分泌的 64kDa 血浆糖蛋白。Fetuin, Fetal Bovine Serum 抑制胰蛋白酶活性并促进细胞附着,生长和分化。

Fetuin, Fetal Bovine Serumamp;;

Fetuin, Fetal Bovine Serum Chemical Structure

CAS No. : 9014-81-7

规格 价格 是否有货 数量
50 mg ¥1100 In-stock
100 mg ¥1900 In-stock
250 mg ¥3200 In-stock
500 mg ¥5500 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

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Fetuin, Fetal Bovine Serum 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

Fetuin, Fetal Bovine Serum is a liver-secreted 64 kDa plasma glycoprotein isolated from fetal bovine serum. Fetuin, Fetal Bovine Serum inhibits trypsin activity and promote cellular attachment, growth, and differentiation[1][2].

体外研究
(In Vitro)

Fetuin, Fetal Bovine Serum is often an important supplement to many serum-free media[2].
Fetuin is an acidic glycoprotein with three N-linked and three O-linked oligosaccharide chains, whose terminal sugar residues are rich in sialic acid (N-acetylneuraminic acid), contributing to its net negative charge[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号

9014-81-7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

H2O : 55 mg/mL (Need ultrasonic)

参考文献
  • [1]. Reiko Furugen, et al. Association of low fetuin-A levels with periodontitis in community-dwelling adults. J Oral Sci. 2020;62(1):67-69.

    [2]. Z Nie , et al. Fetuin: its enigmatic property of growth promotion. Am J Physiol. 1992 Sep;263(3 Pt 1):C551-62.

    [3]. H Wang, et al. Fetuin (alpha2-HS-glycoprotein) opsonizes cationic macrophagedeactivating molecules. Proc Natl Acad Sci U S A. 1998 Nov 24;95(24):14429-34.

CRF, bovine TFA(Synonyms: Corticotropin Releasing Factor bovine TFA)

CRF, bovine TFA;(Synonyms: Corticotropin Releasing Factor bovine TFA) 纯度: 96.50%

CRF, bovine (TFA) 是一种有效的 CRF 受体激动剂,能够取代 [125I-Tyr]ovine CRF,Ki 值为 3.52 nM。

CRF, bovine TFAamp;;(Synonyms: Corticotropin Releasing Factor bovine TFA)

CRF, bovine TFA Chemical Structure

规格 价格 是否有货 数量
500 μg ¥2160 In-stock
1 mg ¥3480 In-stock
5 mg ¥11880 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

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CRF, bovine TFA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

CRF, bovine (TFA) is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM[1].

IC50 Target

Ki: 3.52 nM (CRF receptor)[1].

体外研究
(In Vitro)

CRF, bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM[1]. CRF shows pEC50s of 11.16, 8.53 and 8.70 for human CRF1, human CRF2 and rat CRF[2]. CRF is released from hypothalamic-pituitary-adrenal (HPA) axis induced by stress, and leads to production of glucocorticoids which down regulate immune responses. CRF also has proinflammatory effects. CRF affects brain microvessel endothelial cells (BMEC) structure or function, CRF (100 nM) significantly increases cAMP in BMEC[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

4811.36

Formula

C208H341F3N60O65S

Sequence

Ser-Gln-Glu-Pro-Pro-Ile-Ser-Leu-Asp-Leu-Thr-Phe-His-Leu-Leu-Arg-Glu-Val-Leu-Glu-Met-Thr-Lys-Ala-Asp-Gln-Leu-Ala-Gln-Gln-Ala-His-Asn-Asn-Arg-Lys-Leu-Leu-Asp-Ile-Ala-NH2

Sequence Shortening

SQEPPISLDLTFHLLREVLEMTKADQLAQQAHNNRKLLDIA-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献
  • [1]. CRF, bovine is a potent agonist of CRF receptor, and displaces [125I-Tyr]ovine CRF with a Ki of 3.52 nM[1]. CRF shows pEC50s of 11.16, 8.53 and 8.70 for human CRF1, human CRF2 and rat CRF[2]. CRF is released from hypothalamic-pituitary-adrenal (HPA) axis induced by stress, and leads to production of glucocorticoids which down regulate immune responses. CRF also has proinflammatory effects. CRF affects brain microvessel endothelial cells (BMEC) structure or function, CRF (100 nM) significantly increases cAMP in BMEC[3].

    [2]. Smart D, et al. Characterisation using microphysiometry of CRF receptor pharmacology. Eur J Pharmacol. 1999 Aug 27;379(2-3):229-35.

    [3]. Esposito P, et al. Corticotropin-releasing factor (CRF) can directly affect brain microvessel endothelial cells. Brain Res. 2003 Apr 11;968(2):192-8.