H2DCFDA(Synonyms: DCFH-DA 2′,7′-Dichlorodihydrofluorescein diacetate)

H2DCFDA;(Synonyms: DCFH-DA; 2′,7′-Dichlorodihydrofluorescein diacetate) 纯度: 99.81%

H2DCFDA (DCFH-DA) 是一种可渗透细胞的,用于检测细胞内活性氧 (ROS) 的探针 (Ex/Em=488/525 nm)。

H2DCFDAamp;;(Synonyms: DCFH-DA;  2

H2DCFDA Chemical Structure

CAS No. : 4091-99-0

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ¥550 In-stock
50 mg ¥500 In-stock
100 mg ¥700 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

H2DCFDA 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Immunology/Inflammation Compound Library
  • Metabolism/Protease Compound Library
  • NF-kappa;B Signaling Compound Library
  • Stem Cell Signaling Compound Library
  • Anti-Aging Compound Library
  • Antioxidants Compound Library
  • Oxygen Sensing Compound Library
  • Ferroptosis Compound Library
  • Pyroptosis Compound Library
  • Mitochondria-Targeted Compound Library

生物活性

H2DCFDA (DCFH-DA) is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm)[1].

体外研究
(In Vitro)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are incubated with 5 µM H2DCFDA solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer.
3. Along with the H2DCFDA probe, if indicated, use ROS-insensitive modification of the fluorescein dye DCFDA as a positive control. The staining procedure is the same as for the H2DCFDA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

487.29

Formula

C24H16Cl2O7

CAS 号

4091-99-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

溶解性数据
In Vitro:;

DMSO : 125 mg/mL (256.52 mM; Need ultrasonic)

Ethanol : 20 mg/mL (41.04 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.0522 mL 10.2608 mL 20.5217 mL
5 mM 0.4104 mL 2.0522 mL 4.1043 mL
10 mM 0.2052 mL 1.0261 mL 2.0522 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.08 mg/mL (4.27 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.08 mg/mL (4.27 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.27 mM,饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中,混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中,再用生理盐水定容至 10 mL,完全溶解,澄清透明
  • 3.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.27 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (4.27 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769.

Kinase Assay

ROS Measurements[1]
For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献
  • [1]. Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769.

DAF-FM DA(Synonyms: Diaminofluorescein-FM diacetate)

DAF-FM DA;(Synonyms: Diaminofluorescein-FM diacetate) 纯度: ge;98.0%

DAF-FM DA是低浓度NO检测和定量试剂,DAF-FM的荧光信号能被荧光检测仪器检测到,包括流式细胞仪,荧光显微镜,荧光酶标仪等。

DAF-FM DAamp;;(Synonyms: Diaminofluorescein-FM diacetate)

DAF-FM DA Chemical Structure

CAS No. : 254109-22-3

规格 价格 是否有货 数量
100 μg ¥1800 In-stock

* Please select Quantity before adding items.

生物活性

DAF-FM DA is a reagent to detect and quantify low concentrations of nitric oxide (NO); DAF-FM fluorescence can be detected by any instrument that can detect fluorescein, including flow cytometers, microscopes, fluorescent microplate readers and fluorometers.

分子量

496.42

Formula

C25H18F2N2O7

CAS 号

254109-22-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light)

DMT-dA(bz) Phosphoramidite(Synonyms: DA-CE phosphoramidite)

生化分析试剂 Biochemical Assay Reagents
DMT-dA(bz) Phosphoramidite;(Synonyms: DA-CE phosphoramidite) 纯度: 99.00%

DMT-dA(bz) Phosphoramidite 通常用于合成 DNA。

DMT-dA(bz) Phosphoramidite(Synonyms: DA-CE phosphoramidite)

DMT-dA(bz) Phosphoramidite Chemical Structure

CAS No. : 98796-53-3

规格 价格 是否有货 数量
500 mg ¥500 In-stock
1 g ; 询价 ;
5 g ; 询价 ;

* Please select Quantity before adding items.

DMT-dA(bz) Phosphoramidite 相关产品

bull;相关化合物库:

  • Covalent Screening Library Plus
  • Bioactive Compound Library Plus
  • Cell Cycle/DNA Damage Compound Library
  • Anti-Aging Compound Library
  • Covalent Screening Library
  • Nucleotide Compound Library

生物活性

DMT-dA(bz) Phosphoramidite is typically used in the synthesis of DNA[1].

分子量

857.93

Formula

C47H52N7O7P

CAS 号

98796-53-3

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20deg;C, protect from light, stored under nitrogen

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

溶解性数据
In Vitro:;

DMSO : 25 mg/mL (29.14 mM; ultrasonic and warming and heat to 80°C)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1656 mL 5.8280 mL 11.6560 mL
5 mM 0.2331 mL 1.1656 mL 2.3312 mL
10 mM 0.1166 mL 0.5828 mL 1.1656 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: 2.5 mg/mL (2.91 mM); Suspended solution; Need ultrasonic

    此方案可获得 2.5 mg/mL (2.91 mM) 的均匀悬浊液,悬浊液可用于口服和腹腔注射。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中,混合均匀;向上述体系中加入50 μL Tween-80,混合均匀;然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠,完全溶解于 100 mL ddH₂O 中,得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.91 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.91 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. James D Thorpe, et al. Mechanochemical Synthesis of Short DNA Fragments. Chemistry. 2020 Jul 22;26(41):8857-8861.

荧光染料DAF-2DA(Synonyms: 5,6-Diaminofluorescein diacetat)

荧光染料Dye Reagents DAF-2DA;(Synonyms: 5,6-Diaminofluorescein diacetat)

DAF-2DA (5,6-Diaminofluorescein diacetat) 是测量 NO 最广泛使用的一种荧光探针。

DAF-2DA(Synonyms: 5,6-Diaminofluorescein diacetat)

DAF-2DA Chemical Structure

CAS No. : 205391-02-2

规格 是否有货
50 μg 询价

* Please select Quantity before adding items.

生物活性

DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.

体外研究
(In Vitro)

Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

446.41

Formula

C24H18N2O7

CAS 号

205391-02-2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献
  • [1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.

Destruxin A

Destruxin A;

Destruxin A(DA)是来源昆虫病原真菌 Metarhizium anisopliae 的环肽霉菌毒素,具有杀虫、抗病毒和抗增殖活性。

Destruxin Aamp;;

Destruxin A Chemical Structure

CAS No. : 6686-70-0

规格 价格 是否有货
1 mg ¥8500 询问价格 货期

* Please select Quantity before adding items.

生物活性

Destruxin A (DA) is a cyclo-peptidic mycotoxin from the entomopathogenic fungus Metarhizium anisopliae, with insecticidal, anti-viral and antiproliferative activities[1].

分子量

577.71

Formula

C29H47N5O7

CAS 号

6686-70-0

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -80deg;C 2 years
-20deg;C 1 year
In solvent -80deg;C 6 months
-20deg;C 1 month
参考文献
  • [1]. Gong L, et al. Gene expression profile of Bombyx mori hemocyte under the stress of destruxin A. PLoS One. 2014 May 6;9(5):e96170.

    [2]. Pal S, et al. Fungal peptide Destruxin A plays a specific role in suppressing the innate immune response in Drosophila melanogaster. J Biol Chem. 2007 Mar 23;282(12):8969-77.

    [3]. Odier F, et al. In vitro effect of fungal cyclodepsipeptides on leukemic cells: study of destruxins A, B and E. Biol Cell. 1992;74(3):267-71.

Dmt-2’fluoro-da(bz) amidite

生化分析试剂 Biochemical Assay Reagents
Dmt-2’fluoro-da(bz) amidite; 纯度: ge;97.0%

Dmt-2’fluoro-da(bz) amidite,一种均一修饰的 2′-脱氧-2′-氟代硫代磷酸酯寡核苷酸,是抗核酸酶的反义化合物,对 RNA 靶标具有很高的亲和力和特异性。Dmt-2’fluoro-da(bz) amidite 也是 5′-DMT-3′-亚磷酰胺合成的中间体。

Dmt-2

Dmt-2’fluoro-da(bz) amidite Chemical Structure

CAS No. : 136834-22-5

规格 价格 是否有货 数量
100 mg ¥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Dmt-2’fluoro-da(bz) amidite 相关产品

bull;相关化合物库:

  • Covalent Screening Library Plus
  • Bioactive Compound Library Plus
  • Cell Cycle/DNA Damage Compound Library
  • Anti-Aging Compound Library
  • Covalent Screening Library
  • Nucleotide Compound Library

生物活性

Dmt-2’fluoro-da(bz) amidite, an uniformly modified 2′-deoxy-2′-fluoro phosphorothioate oligonucleotide, is a nuclease-resistant antisense compound with high affinity and specificity for RNA targets. Dmt-2’fluoro-da(bz) amidite is also an intermediate for 5’-DMT-3’-phosphoramidite synthesis[1].

分子量

875.92

Formula

C47H51FN7O7P

CAS 号

136834-22-5

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 100 mg/mL (114.17 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1417 mL 5.7083 mL 11.4166 mL
5 mM 0.2283 mL 1.1417 mL 2.2833 mL
10 mM 0.1142 mL 0.5708 mL 1.1417 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液,再依次添加助溶剂:

——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议您现用现配,当天使用; 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂:;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (2.85 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (2.85 mM,饱和度未知) 的澄清溶液,此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例,取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中,混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献
  • [1]. Kawasaki AM, et, al. Uniformly modified 2′-deoxy-2′-fluoro phosphorothioate oligonucleotides as nuclease-resistant antisense compounds with high affinity and specificity for RNA targets. J Med Chem. 1993 Apr 2;36(7):831-41.

DMT-2′-F-Bz-dA

生化分析试剂 Biochemical Assay Reagents
DMT-2′-F-Bz-dA;

DMT-2′-F-Bz-dA 可用于核苷酸和核酸的合成。

DMT-2

DMT-2′-F-Bz-dA Chemical Structure

CAS No. : 136834-21-4

规格 价格 是否有货 数量
100 mg ¥500 In-stock
250 mg ¥800 In-stock
500 mg ; 询价 ;
1 g ; 询价 ;

* Please select Quantity before adding items.

DMT-2′-F-Bz-dA 相关产品

bull;相关化合物库:

  • Covalent Screening Library Plus
  • Bioactive Compound Library Plus
  • Covalent Screening Library

生物活性

DMT-2′-F-Bz-dA can be used in the synthesis of nucleotides and nucleic acids.

分子量

675.70

Formula

C38H34FN5O6

CAS 号

136834-21-4

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month

DA-JC4

DA-JC4; 纯度: 96.57%

DA-JC4 是一种双 GLP-1/GIP receptor 激动剂,可用于神经系统疾病和胰岛素信号通路的研究。

DA-JC4amp;;

DA-JC4 Chemical Structure

CAS No. : 2315504-40-4

规格 价格 是否有货 数量
5 mg ¥9500 In-stock
10 mg ; 询价 ;
50 mg ; 询价 ;

* Please select Quantity before adding items.

DA-JC4 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Peptide Library

生物活性

DA-JC4 is a dual GLP-1/GIP receptor agonist and can be used for the research of neurological disease and insulin signaling pathways[1][2][3].

IC50 Target

GLP-1/GIP[1]

体外研究
(In Vitro)

DA-JC4 (1~100 nM; hippocampal cells) inhibits rotenone-induced hippocampal neuron death and significantly suppresses Cyt C, Bax and Caspase activation[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究
(In Vivo)

DA-JC4 (10 nmol/kg; i.p.; once-daily for 14 days) significantly prevents spatial learning deficits in a Y- maze test and Morris water maze tests, and decreases phosphorylated tau levels in the rat cerebral cortex and hippocampus[1].
DA-JC4 (25 nmol/kg; i.p.; 6 days) shows high levels expression of tyrosine Hydroxylase in the s. nigra and increases expression of neuroprotective growth factor Glial-Derived Neurotrophic Factor (GDNF)[2].
DA-JC4 (50 nmol/kg; i.p.; once-daily for 7 days) improves Parkinson’s disease symptom potentially and enhances neurotransmission[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Sprague-Dawley rats (210–230 g)
Dosage: 10 nmol/kg
Administration: I.p.
Result: Significantly prevented spatial learning deficits in a Y- maze test and Morris water maze tests, and decreased phosphorylated tau levels in the rat cerebral cortex and hippocampus.
Animal Model: Adult male C57BL/6 mice (8 week-old)
Dosage: 25 nmol/kg/day
Administration: I.p.
Result: Showed high levels expression of tyrosine Hydroxylase in the s. nigra and increased expression of neuroprotective growth factor Glial-Derived Neurotrophic Factor (GDNF).
Animal Model: Adult male Sprague-Dawley (SD) rats (230-280 g)
Dosage: 50 nmol/kg
Administration: I.p.
Result: Improved Parkinson’s disease symptom potentially and enhanced neurotransmission.

分子量

4875.49

Formula

C225H346N56O65

CAS 号

2315504-40-4

Sequence Shortening

Y-{aminoisobutyric acid}-EGTFTSDYSIYLDKQAA-{aminoisobutyric acid}-EFVNWLLAGGPSSGAPPPSKKKKKK-NH2

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Protect from light, stored under nitrogen

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)

参考文献
  • [1]. Shi L, et al. A novel dual GLP-1/GIP receptor agonist alleviates cognitive decline by re-sensitizing insulin signaling in the Alzheimer icv. STZ rat model. Behav Brain Res. 2017;327:65-74.

    [2]. Feng P, et al. Two novel dual GLP-1/GIP receptor agonists are neuroprotective in the MPTP mouse model of Parkinson’s disease. Neuropharmacology. 2018;133:385-394.

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