DIDS sodium salt (MDL101114ZA) is a dual ABCA1 and VDAC1 inhibitor.

ABCA1^[1], VDAC1^[2].

Concentration less than 400 μM DIDS has no evident cytotoxic effect on cell viability Pre-treatment with DIDS at the concentration of 100 and 200 μM result in a prevented effect on ALA-SDT-induced cell death, while dose at 50 μM has no inhibition effect. At the concentration of 400 μM DIDS itself slightly decreases the cell viability, although there is no significant statistic difference as compared with the untreated control. Pre-treatment with DIDS (100 μM) clearly inhibit caspase-3 and caspase-9 activation^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

498.48

C₁₆H₈N₂Na₂O₆S₄

67483-13-0

Room temperature in continental US; may vary elsewhere.

-20deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)

In Vitro:;

DMSO : 50 mg/mL (100.30 mM; Need ultrasonic)

H₂O : 33.33 mg/mL (66.86 mM; Need ultrasonic)

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

  Solubility: ≥ 2.08 mg/mL (4.17 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (4.17 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

  将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

• 2.

  请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 2.08 mg/mL (4.17 mM); Clear solution

  此方案可获得 ≥ 2.08 mg/mL (4.17 mM，饱和度未知) 的澄清溶液。

  以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

  将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明

• [1]. Tsou CY, et al. Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis. Acta Physiol (Oxf). 2014 Apr;210(4):799-810.

  [2]. Chen H, et al. Inhibition of VDAC1 prevents Ca²⁺-mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages. Apoptosis. 2014 Dec;19(12):1712-26.

Cells (5×10⁵ cells/mL) are seeded in the 35-mm Petri dishes and cultured for 72 h at 37°C. Cells are pre-incubated with ALA, and then DIDS of diverse concentrations (0-200 μM) is added before exposed to ultrasound. Cell apoptosis is detected. Briefly, seeded cells (5×10⁵ cells/mL) are pre-treated with test compounds at the indicated concentrations for 1 h (DIDS and NAC), 1.5 h (BAPTA) or with siRNA and exposed to ultrasound. Six hours after ALA-SDT treatment, cells are collected^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

• [1]. Tsou CY, et al. Activation of soluble guanylyl cyclase prevents foam cell formation and atherosclerosis. Acta Physiol (Oxf). 2014 Apr;210(4):799-810.

  [2]. Chen H, et al. Inhibition of VDAC1 prevents Ca²⁺-mediated oxidative stress and apoptosis induced by 5-aminolevulinic acid mediated sonodynamic therapy in THP-1 macrophages. Apoptosis. 2014 Dec;19(12):1712-26.