标签归档：ela

ELA-32(human) TFA

发表于由

ELA-32(human) TFA; 纯度: 99.27%

ELA-32(human) TFA is a potent, high affinity apelin receptor agonist (IC50=0.27 nM; Kd=0.51 nM). ELA-32(human) TFA exhibits no binding GPR15 and GPR25. ELA-32(human) TFA activates the PI3K/AKT pathway and promotes self-renewal of hESCs via cell-cycle progression and protein translation. ELA-32(human) TFA also potentiates the TGFβ pathway, priming hESCs toward the endoderm lineage. ELA-32(human) TFA stimulates angiogenesis in HUVEC cells.

ELA-32(human) TFAamp;;

ELA-32(human) TFA Chemical Structure

规格 价格 是否有货 数量
5 mg ￥6500 In-stock
10 mg ￥9800 In-stock
50 mg ; 询价 ;
100 mg ; 询价 ;

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生物活性

ELA-32(human) TFA is a potent, high affinity apelin receptor agonist (IC50=0.27 nM; Kd=0.51 nM). ELA-32(human) TFA exhibits no binding GPR15 and GPR25. ELA-32(human) TFA activates the PI3K/AKT pathway and promotes self-renewal of hESCs via cell-cycle progression and protein translation. ELA-32(human) TFA also potentiates the TGFβ pathway, priming hESCs toward the endoderm lineage. ELA-32(human) TFA stimulates angiogenesis in HUVEC cells.

分子量

4081.84

Formula

C172H290F3N63O41S4
Sequence Shortening

QRPVNLTMRRKLRKHNCLQRRCMPLHSRVPFP (Disulfide bridge: Cys17-Cys22)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Protect from light, stored under nitrogen

Powder -80deg;C 2 years
-20deg;C 1 year

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (protect from light, stored under nitrogen)
溶解性数据
In Vitro:;

H2O : 100 mg/mL (24.50 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 0.2450 mL 1.2249 mL 2.4499 mL
5 mM 0.0490 mL 0.2450 mL 0.4900 mL
10 mM 0.0245 mL 0.1225 mL 0.2450 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

ELA RR>GG(Synonyms: ELA-32 negative control)

发表于由

ELA RR>GG;(Synonyms: ELA-32 negative control)

ELA RR>GG (ELA-32 negative control) 是一种 ELABELA 的突变体多肽。ELA RR>GG 可作为 ELABELA (HY-P2196) 的阴性对照。

ELA RRgt;GGamp;;(Synonyms: ELA-32 negative control)

ELA RR>GG Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

ELA RR>GG (ELA-32 negative control), an ELABELA (ELA-32 human) mutant peptide, is inactive. ELA RR>GG is a negative control for ELABELA (HY-P2196)[1].

分子量

3769.55

Formula

C162H271N57O39S4
Sequence

Gln-Arg-Pro-Val-Asn-Leu-Thr-Met-Gly-Gly-Lys-Leu-Arg-Lys-His-Asn-Cys-Leu-Gln-Arg-Arg-Cys-Met-Pro-Leu-His-Ser-Arg-Val-Pro-Phe-Pro (Disulfide bridge: Cys17-Cys22)
Sequence Shortening

QRPVNLTMGGKLRKHNCLQRRCMPLHSRVPFP (Disulfide bridge: Cys17-Cys22)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Ho L, et al. ELABELA Is an Endogenous Growth Factor that Sustains hESC Self-Renewal via the PI3K/AKT Pathway [published correction appears in Cell Stem Cell. 2015 Nov 5;17(5):635. Sczerbinska, Iwona [corrected to Szczerbinska, Iwona]]. Cell Stem Cell. 2015;17(4):435-447.

ELA-32(human)

发表于由

ELA-32(human);

ELA-32 (human) 是一种有效的关键心脏发育肽，通过 G-protein–coupled apelin 受体发挥作用。

ELA-32(human)amp;;

ELA-32(human) Chemical Structure

CAS No. : 1680205-79-1

规格 是否有货
1 mg 询价
5 mg 询价
10 mg 询价

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生物活性

ELA-32 (human) is a potent critical cardiac developmental peptide that acts through the G-protein–coupled apelin receptor[1].

分子量

3967.82

Formula

C170H289N63O39S4
CAS 号

1680205-79-1
Sequence Shortening

QRPVNLTMRRKLRKHNCLQRRCMPLHSRVPFP (Disulfide bridge: Cys17-Cys22)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Yang P, et al. Elabela/Toddler Is an Endogenous Agonist of the Apelin APJ Receptor in the Adult Cardiovascular System, and Exogenous Administration of the Peptide Compensates for the Downregulation of Its Expression in Pulmonary Arterial Hypertension. Circulation. 2017;135(12):1160-1173.

ELA-11(human)

发表于由

ELA-11(human);

ELA-11(human)，一种多肽，是人类 apelin 受体的完全激动剂，pKi 值为 7.85。ELA-11(human) 可完全抑制 Forskolin 诱导的 cAMP 产生并刺激 β-arrestin 募集。

ELA-11(human)amp;;

ELA-11(human) Chemical Structure

CAS No. : 1784687-32-6

规格 是否有货
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250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

ELA-11(human), a peptide, is a full agonist of human apelin receptor, with a pKi of 7.85. ELA-11(human) completely inhibits Forskolin-induced cAMP production and stimulates β-arrestin recruitment[1].

IC50 Target

pKi: 7.85 (apelin receptor)[1]
体外研究
(In Vitro)

ELA-11(human) (1 pM-100 nM) completely inhibits Forskolin-induced cAMP production in a concentration-dependent manner[1].
ELA-11(human) (1 pM-1 μM) stimulates β-arrestin recruitment in a concentration-dependent manner with subnanomolar potencies[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

1283.56

Formula

C58H90N16O13S2
CAS 号

1784687-32-6
Sequence Shortening

CMPLHSRVPFP
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Yang P, et, al. Elabela/Toddler Is an Endogenous Agonist of the Apelin APJ Receptor in the Adult Cardiovascular System, and Exogenous Administration of the Peptide Compensates for the Downregulation of Its Expression in Pulmonary Arterial Hypertension. Circulation. 2017 Mar 21;135(12):1160-1173.

ELA-11(human) TFA

发表于由

ELA-11(human) TFA;

ELA-11(human) TFA is a high affinity apelin receptor agonist (Ki=14 nM). ELA-11(human) TFA is a bioactive fragment of ELA-32. ELA-11(human) TFA inhibits forskolin-induced cAMP production and stimulates β-arrestin recruitment in vitro.

ELA-11(human) TFAamp;;

ELA-11(human) TFA Chemical Structure

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

ELA-11(human) TFA is a high affinity apelin receptor agonist (Ki=14 nM). ELA-11(human) TFA is a bioactive fragment of ELA-32. ELA-11(human) TFA inhibits forskolin-induced cAMP production and stimulates β-arrestin recruitment in vitro.

分子量

1397.58

Formula

C60H91F3N16O15S2
Sequence Shortening

CMPLHSRVPFP
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

ELA-21 (human)

发表于由

ELA-21 (human);

ELA-21 (human) 是一种 apelin 受体激动剂，pKi 为 8.52。ELA-21 (human) 在亚纳摩尔效价下，完全抑制 Forskolin 诱导的 cAMP 产生，并刺激 β-arrestin 募集。ELA-21 (human) 也是 G 蛋白依赖性和非依赖性途径的激动剂。

ELA-21 (human)amp;;

ELA-21 (human) Chemical Structure

CAS No. : 2245073-05-4

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

ELA-21 (human) is an apelin receptor agonist with a pKi of 8.52. ELA-21 (human) completely inhibits Forskolin-induced cAMP production and stimulates β-arrestin recruitment with subnanomolar potencies. ELA-21 (human) is an agonist in G-protein-dependent and -independent pathways[1].

分子量

2587.14

Formula

C112H184N40O25S3
CAS 号

2245073-05-4
Sequence

Leu-Arg-Lys-His-Asn-Cys-Leu-Gln-Arg-Arg-Cys-Met-Pro-Leu-His-Ser-Arg-Val-Pro-Phe-Pro (Disulfide bridge: Cys6-Cys11)
Sequence Shortening

LRKHNCLQRRCMPLHSRVPFP (Disulfide bridge: Cys6-Cys11)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Peiran Yang, et al. Elabela/Toddler Is an Endogenous Agonist of the Apelin APJ Receptor in the Adult Cardiovascular System, and Exogenous Administration of the Peptide Compensates for the Downregulation of Its Expression in Pulmonary Arterial Hypertension. Circulation. 2017 Mar 21;135(12):1160-1173.

ELA-14 negative control

发表于由

ELA-14 negative control;

ELA-14 negative control 是一种多肽。ELA-14 negative control 可作为 ELA-14 的阴性对照。

ELA-14 negative controlamp;;

ELA-14 negative control Chemical Structure

CAS No. : 1885873-44-8

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

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生物活性

ELA-14 negative control, a peptide, is inactive. ELA-14 negative control is a negative control for ELA-14[1].

分子量

1621.93

Formula

C72H112N22O17S2
CAS 号

1885873-44-8
Sequence Shortening

{Glp}-RRCMPLHSAVPFP
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent Solubility
In Vitro:;

H2O

Peptide Solubility and Storage Guidelines:

1.;;Calculate the length of the peptide.

2.;;Calculate the overall charge of the entire peptide according to the following table:

; Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.;;Recommended solution:

Overall charge of peptide Details
Negative (lt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, add NH4OH (lt;50 μL).
3.;;If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (gt;0) 1.;;Try to dissolve the peptide in water first.
2.;;If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.;;If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.;;Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.;;For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
参考文献

  • [1]. Murza A, et, al. Discovery and Structure-Activity Relationship of a Bioactive Fragment of ELABELA that Modulates Vascular and Cardiac Functions. J Med Chem. 2016 Apr 14;59(7):2962-72.