标签归档：black

荧光染料Brilliant Black BN(Synonyms: E 151)

发表于由

荧光染料Dye Reagents Brilliant Black BN;(Synonyms: E 151) 纯度: ge;98.0%

Brilliant black BN (E151) 是一种偶氮染料 dye 和一种食品着色剂。Brilliant black BN (E151) 是一种很有前途的抗病毒试剂 (antiviral agent )，通过抑制 EV71 与其细胞脱衣因子亲环素a的相互作用来对抗 EV71 感染。Brilliant black BN (E151) 有潜力用于传染性疾病的相关研究。

Brilliant Black BNamp;;(Synonyms: E 151)

Brilliant Black BN Chemical Structure

CAS No. : 2519-30-4

规格 价格 是否有货 数量
10;mM;*;1 mL in DMSO ￥1490 In-stock
25 mg ￥1350 In-stock
100 mg ￥3950 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Brilliant Black BN 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Anti-Infection Compound Library
  • Antiviral Compound Library

生物活性

Brilliant black BN (E151) is an azo dye and a food colorant[1]. Brilliant black BN is a promising antiviral agent against EV71 infection via inhibiting the interaction between EV71 and its cellular uncoating factor cyclophilin A. Brilliant black BN has the potential for the investigation of contagious disease[2].

IC50 Target

IC50: 2.39 µM- 28.12 µM (various strains of EV71)[2]
体外研究
(In Vitro)

Brilliant black BN (E151) inhibits the interaction between EV71 and its cellular uncoating factor cyclophilin A [1].
Brilliant black BN (E151) inhibited the infection EV71 -GFP at a concentration of 300 µM as evidenced from the reduced GFP signals. Brilliant black BN (E151)exhibits the inhibition of EV71 -GFP as a dose-dependent manner in infected RD cells, exhibits an IC50 value of 10.1 µM[1].
Brilliant black BN (E151) is able to inhibit all tested 28 EV71, CVA16 and CVA6 strains. In rhabdomyosarcoma cells, 50% inhibitory concentration 29 of the dye E151 for various strains of EV71 ranged from 2.39 µM to 28.12 µM ,whereas its 50% cytotoxic concentration is 1870 µM [1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Brilliant black BN (E151)(intraperitoneal injection; 200 mg/kg; 14 days)exhibits mild illness with clinical scores less than 3 and subsequently recovered when compared with PBS-group.All E151 270 treated mice are completely protected throughout the experiment[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 14-days old AG129 mice with EV71[1]
Dosage: 200 mg/kg
Administration: Intraperitoneal injection
Result: Had a Protective eddect on EV71 challenged AG129 mice by dye E151.

分子量

867.68

Formula

C28H17N5Na4O14S4
CAS 号

2519-30-4
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 125 mg/mL (144.06 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.1525 mL 5.7625 mL 11.5250 mL
5 mM 0.2305 mL 1.1525 mL 2.3050 mL
10 mM 0.1152 mL 0.5762 mL 1.1525 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。
参考文献

  • [1]. Lang W, et al. Biodecolorization of a food azo dye by the deep sea Dermacoccus abyssi MT1.1(T) strain from the Mariana Trench.J Environ Manage. 2014 Jan;132:155-64.

    [2]. Meng T, et al. In Vitro and In Vivo Inhibition of the Infectivity of Human Enterovirus 71 by a Sulfonated Food Azo Dye, Brilliant Black BN.J Virol. 2019 Aug 13;93(17).

Hematoxylin(Synonyms: 苏木精 Natural Black 1 Haematoxylin)

发表于由

Hematoxylin;(Synonyms: 苏木精; Natural Black 1; Haematoxylin) 纯度: ge;98.0%

Hematoxylin (Natural Black 1)，一种天然存在的类黄酮化合物，衍生自木柴树 Haematoxylon campechianum。Hematoxylin 是一种组织学上的核染色剂，也是有效的 Aβ42 原纤维形成的抑制剂，IC50 为 1.6 µM。

Hematoxylinamp;;(Synonyms: 苏木精; Natural Black 1; Haematoxylin)

Hematoxylin Chemical Structure

CAS No. : 517-28-2

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥500 In-stock
100 mg ￥450 In-stock
1 g ￥600 In-stock
5 g ￥800 In-stock
10 g ; 询价 ;
50 g ; 询价 ;

* Please select Quantity before adding items.

Hematoxylin 相关产品

bull;相关化合物库:

  • Natural Product Library
  • Phenols Library
  • FDA Approved amp; Pharmacopeial Drug Library
  • Anti-Alzheimer’s Disease Compound Library
  • Neurodegenerative Disease-related Compound Library
  • Natural Product Library Plus
  • Bioactive Compound Library Plus
  • Neuronal Signaling Compound Library

生物活性

Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from the logwood tree, Haematoxylon campechianum. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC50 of 1.6 µM.

IC50 Target

IC50: 1.6 µM (Aβ42 fibrillogenesis)[2]
体外研究
(In Vitro)

When exposed to air, Hematoxylin is oxidized to reddish brown hematein. When oxidized to its hematein form and combined with a mordant, usually a metal salt, Hematoxylin stains tissue sections a deep blue to black color depending on the staining method. By itself, Hematoxylin is also amphoteric in its hematein form; it is red at acid pH and blue at alkaline pH. Differentiation following Hematoxylin staining removes nonspecific staining[1].
Hematoxylin treatment greatly alleviates Aβ42-induced cytotoxicity in SH-SY5Y cells. Hematoxylin is a potential agent against Aβ fibrillogenesis and cytotoxicity[2].
The Hematoxylin and Eosin (HE) stained tissue section is the cornerstone of anatomical pathology diagnosis. The HE procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
体内研究
(In Vivo)

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The method of HE staining[4]:
1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change.
2. Hydrate the samples as follows.
i. Transfer the slides through three changes of 100% ethanol for 2 min per change.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer to 70% ethanol for 2 min.
iv. Rinse the slides in running tap water at room temperature for at least 2 min.
3. Stain the samples in Hematoxylin solution for 3 min.
4. Place the slides under running tap water at room temperature for at least 5 min.
5. Stain the samples in working eosin Y solution for 2 min.
6. Dehydrate the samples as follows.
i. Dip the slides in 95% ethanol about 20 times.
ii. Transfer to 95% ethanol for 2 min.
iii. Transfer through two changes of 100% ethanol for 2 min per change.
7. Clear the samples in three changes of xylene for 2 min per change.
8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

302.28

Formula

C16H14O6
CAS 号

517-28-2
中文名称

苏木精；苏木色精；苏木素
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20deg;C 3 years
4deg;C 2 years
In solvent -80deg;C 6 months
-20deg;C 1 month
溶解性数据
In Vitro:;

DMSO : 50 mg/mL (165.41 mM; Need ultrasonic)

H2O : 6.67 mg/mL (22.07 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 3.3082 mL 16.5410 mL 33.0819 mL
5 mM 0.6616 mL 3.3082 mL 6.6164 mL
10 mM 0.3308 mL 1.6541 mL 3.3082 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.27 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.27 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
  • 3.

    请依序添加每种溶剂：;10% DMSO ;; 90% corn oil

    Solubility: ≥ 2.5 mg/mL (8.27 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (8.27 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. M Titford. The long history of hematoxylin. Biotech Histochem. Mar-Apr 2005;80(2):73-8.

    [2]. Yilong Tu, et al. Hematoxylin Inhibits Amyloid β-Protein Fibrillation and Alleviates Amyloid-Induced Cytotoxicity. J Phys Chem B. 2016 Nov 10;120(44):11360-11368.

    [3]. Ada T Feldman, et al. Tissue processing and hematoxylin and eosin staining. Methods Mol Biol. 2014;1180:31-43.

    [4]. Robert D Cardiff, et al. Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc. 2014 Jun 2;2014(6):655-8.

Direct Black 38(Synonyms: Chlorazol Black E Ferristatin II disodium C.I. 30235)

发表于由

Direct Black 38;(Synonyms: Chlorazol Black E; Ferristatin II disodium; C.I. 30235)

Direct Black 38 (Chlorazol Black E) 是一种偶氮染料。Direct Black 38 诱导大鼠肝中非程序性 DNA 合成，也诱导骨髓中形成微核。

Direct Black 38amp;;(Synonyms: Chlorazol Black E; Ferristatin II disodium; C.I. 30235)

Direct Black 38 Chemical Structure

CAS No. : 1937-37-7

规格 价格 是否有货 数量
100 mg ￥700 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Direct Black 38 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus
  • Tumorigenesis Related Compound Library

生物活性

Direct Black 38 (Chlorazol Black E) is an azo dye. Direct Black 38 induces unscheduled DNA synthesis in liver and micronucleus in bone marrow of rats in vivo[1].

分子量

781.73

Formula

C34H25N9Na2O7S2
CAS 号

1937-37-7
中文名称

直接黑 38；偶氮黑 38；氯唑黑 38
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (31.98 mM; Need ultrasonic)

H2O : 5 mg/mL (6.40 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.2792 mL 6.3961 mL 12.7921 mL
5 mM 0.2558 mL 1.2792 mL 2.5584 mL
10 mM 0.1279 mL 0.6396 mL 1.2792 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.20 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

    此方案可获得 ≥ 2.5 mg/mL (3.20 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Beije B. Induction of unscheduled DNA synthesis in liver and micronucleus in bone marrow of rats exposed in vivo to the benzidine-derived azo dye, Direct Black 38. Mutat Res. 1987 Apr;187(4):227-34.

Direct Black 38 free acid(Synonyms: Chlorazol Black E free acid Ferristatin II C.I. 30235 free acid)

发表于由

Direct Black 38 free acid;(Synonyms: Chlorazol Black E free acid; Ferristatin II; C.I. 30235 free acid)

Direct Black 38 free acid 是一种多聚磺化染料。Direct Black 38 free acid 促进转铁蛋白受体-1 (transferrin receptor-1) 在体内外的降解。

Direct Black 38 free acidamp;;(Synonyms: Chlorazol Black E free acid; Ferristatin II; C.I. 30235 free acid)

Direct Black 38 free acid Chemical Structure

CAS No. : 22244-14-0

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

Direct Black 38 free acid 的其他形式现货产品:

Direct Black 38

生物活性

Direct Black 38 free acid is a polysulphonated dye. Direct Black 38 free acid promotes degradation of transferrin receptor-1 in vitro and in vivo[1].

分子量

737.76

Formula

C34H27N9O7S2
CAS 号

22244-14-0
中文名称

直接黑 38 游离酸
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Beije B. Induction of unscheduled DNA synthesis in liver and micronucleus in bone marrow of rats exposed in vivo to the benzidine-derived azo dye, Direct Black 38. Mutat Res. 1987 Apr;187(4):227-34.

    [2]. Byrne SL, et al. Ferristatin II promotes degradation of transferrin receptor-1 in vitro and in vivo. PLoS One. 2013 Jul 23;8(7):e70199.

Sudan Black B(Synonyms: 苏丹黑B)

发表于由

Sudan Black B;(Synonyms: 苏丹黑B)

Sudan Black B 是一种脂溶性重氮染料，是一种组织化学染色剂。Sudan Black B 可用于中性甘油三酯和脂质的染色。

Sudan Black Bamp;;(Synonyms: 苏丹黑B)

Sudan Black B Chemical Structure

CAS No. : 4197-25-5

规格 是否有货
100 mg ; 询价 ;
250 mg ; 询价 ;
500 mg ; 询价 ;

* Please select Quantity before adding items.

生物活性

Sudan Black B, a fat-soluble diazo dye, is a histochemical stain. Sudan Black B can be used for staining of neutral triglycerides and lipids[1].

体外研究
(In Vitro)

Sudan Black B works also in frozen material, and therefore can be complementarily used with SA-β-gal when investigating senescence. Sudan Black B is a highly lipophilic agent that exhibits high affi nity to the lipid compartment of lipofuscin. The positive Sudan Black B-lipofuscin reaction reveals blue-black intracellular granules in cells and frozen tissues and brown to black granules in FFPE tissues under the light microscope.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

456.54

Formula

C29H24N6
CAS 号

4197-25-5
中文名称

苏丹黑B
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Konstantinos Evangelou, et al. Sudan Black B, The Specific Histochemical Stain for Lipofuscin: A Novel Method to Detect Senescent Cells. Methods Mol Biol. 2017;1534:111-119.

Pall Metricel Black圆盘过滤膜, 60138, 66586, 66585, 60065, 66587, 66588

发表于由

名称：Metricel Black圆盘过滤膜

型号：60138, 66586, 66585, 60065, 66587, 66588

简介：Pall 颇尔 Metricel Black圆盘过滤膜, 60138, 66586, 66585, 60065, 66587, 66588

Pall 颇尔 Metricel Black圆盘过滤膜, 60138, 66586, 66585, 60065, 66587, 66588
 
产品简介：
用于微生物分析与颖粒分析的对比膜
．黑底色为在实验室计数不透明的菌落以及监测工艺流体中的浅色颗粒汕口在微电子业中）提供良好的对比度。
．独特的点状网格式样不会促进或抑制菌落生长。
．经认证，可用于膜过滤器（MF ）技术中。
．提供单个无菌包装，用于无菌检测。
 
Pall 颇尔 Metricel Black圆盘过滤膜, 60138, 66586, 66585, 60065, 66587, 66588
 
应用
．用于分离和计算酵母和霉菌的非常好的膜。
．用于食物和饮料质量控制实验室中对腐败生物监测和识别。
．很适合用来监测工艺流体中的颗粒污染，如微电子业。
 
Pall 颇尔 Metricel Black圆盘过滤膜, 60138, 66586, 66585, 60065, 66587, 66588
 
订购信息
产品编号
说明
包装
60138
0.45µm, 25mm，网格
100片／包装
66586
0.45µm, 47mm，网格
100片／包装
66585
0.45µm, 47mm，网格，单片，无菌包装
100片／包装
60065
0.8µm, 25mm，网格
100片／包装
66587
0.8µm, 47mm，网格，单片，无菌包装
100片／包装
66588
0.8µm, 47mm，网格
100片／包装

 

Pall 颇尔 Metricel Black圆盘过滤膜, 60138, 66586, 66585, 60065, 66587, 66588