标签归档：di

CGP7930

发表于由

CGP7930;

CGP7930 (3-(3′,5′-Di-tert-butyl-4′-hydroxy)phenyl-2,2-dimethylpropanol) 是一种代谢型 GABAB 受体变构正向调节剂。CGP7930 增强 l-baclofen 对培养的皮层神经元振荡活动的抑制作用。

CGP7930amp;;

CGP7930 Chemical Structure

CAS No. : 57717-80-3

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生物活性

CGP7930 (3-(3’,5’-Di-tert-butyl-4’-hydroxy) phenyl-2, 2-dimethylpropanol) is a positive metabotropic GABAB receptor allosteric modulator. CGP7930 enhances the inhibitory effect of l-baclofen on the oscillatory activity of cultured cortical neurons[1].

分子量

292.46

Formula

C19H32O2
CAS 号

57717-80-3
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Urwyler S, et al. Positive allosteric modulation of native and recombinant gamma-aminobutyric acid(B) receptors by 2,6-Di-tert-butyl-4-(3-hydroxy-2,2-dimethyl-propyl)-phenol (CGP7930) and its aldehyde analog CGP13501. Mol Pharmacol. 2001; 60(5):963-71.

15,16-Di-O-acetyldarutoside

发表于由

上海金畔生物科技有限公司提供天然产物萜类及其苷类Terpenoids and Glycosides。

15,16-Di-O-acetyldarutoside 

15,16-Di-O-acetyldarutoside (compound 5) 是一种 ent-pimarane 二萜化合物，从 Siegesbeckia orientalis 的乙醇提取物中分离出来。

15,16-Di-O-acetyldarutoside

15,16-Di-O-acetyldarutoside Chemical Structure

CAS No. : 1188282-02-1

规格 是否有货
100 mg   询价  
250 mg   询价  
500 mg   询价  

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生物活性

15,16-Di-O-acetyldarutoside (compound 5) is an ent-pimarane diterpenoid compound isolated from the ethanol extract of Siegesbeckia orientalis[1].

分子量

568.70

Formula

C30H48O10
CAS 号

1188282-02-1
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Wang F, et al. ent-Pimarane diterpenoids from Siegesbeckia orientalis and structure revision of a related compound. J Nat Prod. 2009;72(11):2005-2008.

4′,​7-​Di-​O-​methylnaringenin

发表于由

天然产物 黄酮类 Flavonoids

4′,​7-​Di-​O-​methylnaringenin 

4′,​7-​Di-​O-​methylnaringenin 是山姜属植物中的黄酮类化合物。

4

4′,​7-​Di-​O-​methylnaringenin Chemical Structure

CAS No. : 29424-96-2

规格 价格 是否有货
5 mg ￥3360 询问价格 & 货期
10 mg ￥5710 询问价格 & 货期

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生物活性

4′,​7-​Di-​O-​methylnaringenin is a flavonoid found in Renealmia alpinia[1].

分子量

300.31

Formula

C17H16O5
CAS 号

29424-96-2
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Gómez-Betancur I, et al. Antinociceptive activity of extracts and secondary metabolites from wild growing and micropropagated plants of Renealmia alpinia. J Ethnopharmacol. 2015 May 13;165:191-7.

Fluorescein di(β-D-galactopyranoside)(Synonyms: FDG)

发表于由

Fluorescein di(β-D-galactopyranoside);(Synonyms: FDG)

Fluorescein di(β-D-galactopyranoside) 是 β-半乳糖苷酶的荧光底物 (λex=485 nm，λem=535 nm)。

Fluorescein di(β-D-galactopyranoside)amp;;(Synonyms: FDG)

Fluorescein di(β-D-galactopyranoside) Chemical Structure

CAS No. : 17817-20-8

规格 价格 是否有货
1 mg ￥1500 询问价格 货期

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生物活性

Fluorescein di(β-D-galactopyranoside) is a fluorogenic substrate for β-galactosidase (λex=485 nm, λem=535 nm).

体外研究
(In Vitro)

The fluorescence produced by Fluorescein di(β-D-galactopyranoside) increases in a time- and dose-dependent manner. The level of fluorescence produced by the double-substrate method is much lower than that by the Fluorescein di(β-D-galactopyranoside) method. Results show that the fluorescein produced by Fluorescein di(β-D-galactopyranoside) in Hs68 cells is proportional to the number of passages[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

656.59

Formula

C32H32O15
CAS 号

17817-20-8
中文名称

荧光素二(β-D-吡喃半乳糖苷)
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15;325(2):337-43.
Cell Assay
[1]

The cells (5×103 cells per well) are cultured in a 96-well plate overnight for attachment, washed, and then fixed in solutions. An aliquot (100 μL) of the reaction buffer (i.e., the staining solution without X-Gal) is added into each well. Then, 10 μL of 2 mM Fluorescein di(β-D-galactopyranoside) is added per well and the plate is incubated in the dark at 37°C for 24 h without CO2 supply. After incubation at 37°C for 24 h, 100 μL of the supernatant is transferred to a 96-well plate for fluorescent measurement in triplicates. The fluorescein fluorescence is measured using a fluorometer with an excitation at 485 nm and an emission at 535 nm[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Yang NC, et al. A fluorimetric method using fluorescein di-beta-D-galactopyranoside for quantifying the senescence-associated beta-galactosidase activity in human foreskin fibroblast Hs68 cells. Anal Biochem. 2004 Feb 15;325(2):337-43.

4-Di-16-ASP(Synonyms: DiA)

发表于由

4-Di-16-ASP;(Synonyms: DiA)

4-Di-16-ASP 是一种膜染料。4-Di-16-ASP 是一种亲脂性氨基苯乙烯基探针，具有宽发射光谱（可以用绿色、橙色甚至红色滤光片检测）。常用于神经元膜追踪 (它比 DiO 扩散得更快)。

4-Di-16-ASPamp;;(Synonyms: DiA)

4-Di-16-ASP Chemical Structure

CAS No. : 114041-00-8

规格 是否有货
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生物活性

4-Di-16-ASP is a green fluorescent membrane dye. 4-Di-16-ASP is a lipophilic aminostyryl probe with a broad emission spectrum (can be detected with green, orange or even red filters). It is commonly used for neuronal membrane tracing (it diffuses faster than DiO)[1].

分子量

787.04

Formula

C46H79IN2
CAS 号

114041-00-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Lucas McDonald, et al. An NIR Emitting Styryl Dye with Large Stokes Shift to Enable co-Staining Study on Zebrafish Neuromast Hair Cells. Bioorg Chem. 2019 Aug;89:103040.

Di-8-ANEPPS

发表于由

Di-8-ANEPPS; 纯度: 99.46%

Di-8-ANEPPS 是萘基苯乙烯基电压敏感染料，它们的荧光激发和发射光谱会因电压的变化而变化。

Di-8-ANEPPSamp;;

Di-8-ANEPPS Chemical Structure

CAS No. : 157134-53-7

规格 价格 是否有货 数量
1 mg ￥2700 In-stock
5 mg ; 询价 ;
10 mg ; 询价 ;

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Di-8-ANEPPS 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

体外研究
(In Vitro)

A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

592.87

Formula

C36H52N2O3S
CAS 号

157134-53-7
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

-20deg;C, protect from light

*该产品在溶液状态不稳定，建议您现用现配，即刻使用。
参考文献

  • [1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.

Cell Assay
[1]

To implement the technique, cells enzymatically isolated from mouse flexor digitorum brevis (FDB) muscle are stained by brief exposure to a saline with Di-8-ANEPPS and then washed in solution[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

  • [1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.

2-Di-1-ASP

发表于由

2-Di-1-ASP; 纯度: 99.47%

2-Di-1-ASP (Compound 18a) 是一种单苯乙烯基染料，广泛用作双链 DNA 的线粒体染色和凹槽结合荧光探针。2-Di-1-ASP 对 G-四链体 (G4) 和双链 DNA 具有选择性。

2-Di-1-ASPamp;;

2-Di-1-ASP Chemical Structure

CAS No. : 2156-29-8

规格 价格 是否有货 数量
Free Sample (0.1-0.5 mg) ; Apply now ;
10;mM;*;1 mL in DMSO ￥550 In-stock
100 mg ￥500 In-stock
200 mg ; 询价 ;
500 mg ; 询价 ;

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2-Di-1-ASP 相关产品

bull;相关化合物库:

  • Bioactive Compound Library Plus

生物活性

2-Di-1-ASP (Compound 18a) is a mono-stryryl dye, and widely used as mitochondrial stain and groove-binding fluorescent probes for double-stranded DNA. 2-Di-1-ASP is selective for G-quadruplex (G4) and double-stranded DNA[1].

体外研究
(In Vitro)

2-Di-1-ASP (Compound 18a) displays significant fluorescence enhancements in the presence of G-quadruplex (G4) structures (up to 300-fold), and good selectivity with respect to double-stranded DNA. 2-Di-1-ASP shows fluorimetric selectivity for parallel G4-DNA forms (c-kit2, c-kit87up, c-myc)[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

366.24

Formula

C16H19IN2
CAS 号

2156-29-8
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4deg;C, sealed storage, away from moisture

*In solvent : -80deg;C, 6 months; -20deg;C, 1 month (sealed storage, away from moisture)
溶解性数据
In Vitro:;

DMSO : 25 mg/mL (68.26 mM; Need ultrasonic)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.7304 mL 13.6523 mL 27.3045 mL
5 mM 0.5461 mL 2.7304 mL 5.4609 mL
10 mM 0.2730 mL 1.3652 mL 2.7304 mL

*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂：;10% DMSO ;; 40% PEG300 ;; 5% Tween-80 ;; 45% saline

    Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.68 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。

    将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液

  • 2.

    请依序添加每种溶剂：;10% DMSO ;; 90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (5.68 mM); Clear solution

    此方案可获得 ≥ 2.08 mg/mL (5.68 mM，饱和度未知) 的澄清溶液。

    以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。

    将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
*以上所有助溶剂都可在 MCE 网站选购。
参考文献

  • [1]. Xie X, et al. Identification of optimal fluorescent probes for G-quadruplex nucleic acids through systematic exploration of mono- and distyryl dye libraries. Beilstein J Org Chem. 2019 Aug 6;15:1872-1889.

Korepimedoside C(Synonyms: Epimedin I)

发表于由

天然产物 糖类和糖苷 Saccharides and Glycosides

Korepimedoside C;(Synonyms: Epimedin I)

Korepimedoside C (Epimedin I) 是一种从 Epimedium koreanum Nakai 中分离出的黄酮醇苷。Epimedium koreanum Nakai 是一种中草药，可用于阳痿，骨质疏松，免疫抑制和心血管疾病的研究。

Korepimedoside C(Synonyms: Epimedin I)

Korepimedoside C Chemical Structure

CAS No. : 205445-00-7

规格 是否有货
5 mg 询价
10 mg 询价
25 mg 询价

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生物活性

Korepimedoside C (Epimedin I), a flavonol glycoside, is isolated from the aerial parts of Epimedium koreanum Nakai. Epimedium koreanum Nakai is a famous Chinese herbal medicine for the research of impotence, osteoporosis, immune suppression and cardiovascular diseases[1][2].

分子量

880.84

Formula

C41H52O21
CAS 号

205445-00-7
中文名称

朝藿苷丙
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
参考文献

  • [1]. Zhao Y, et, al. Effect of drying processes on prenylflavonoid content and antioxidant activity of Epimedium koreanum Nakai. J Food Drug Anal. 2018 Apr;26(2):796-806.

    [2]. Zheng XX, et, al. Determination of Korepimedoside C in epimedium from different origin by HPLC. Chemical Engineer. 2015 Jul.