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日本同仁化学Hampton蛋白结晶试剂盒Sodium citrate tribasic dihydrate Buffer/HR2-571/HR2-735| DOJINDO

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Products > Optimize Reagents > Optimize – Buffers > Sodium citrate tribasic dihydrate Buffer

Sodium citrate tribasic dihydrate Buffer

Hampton蛋白结晶试剂盒Sodium citrate tribasic dihydrate Buffer/HR2-571/HR2-735

CAT NO

HR2-571

NAME

1.0 M Sodium citrate tribasic dihydrate

DESCRIPTION

100 mL

PRICE

$56.00

CAT NO

HR2-735

NAME

1.0 M Sodium citrate tribasic dihydrate pH 5.6

DESCRIPTION

100 mL

PRICE

$56.00

支持材料

Hampton蛋白结晶试剂盒Sodium citrate tribasic dihydrate Buffer/HR2-571/HR2-735 HR2-571 Sodium citrate tribasic dihydrate SDSHampton蛋白结晶试剂盒Sodium citrate tribasic dihydrate Buffer/HR2-571/HR2-735 HR2-735 Sodium citrate tribasic dihydrate pH 5.6 SDSHampton蛋白结晶试剂盒Sodium citrate tribasic dihydrate Buffer/HR2-571/HR2-735 Titration Table

应用

  • Crystallization grade Sodium citrate tribasic dihydrate buffer for formulating screens or for optimization
  • 结晶级柠檬酸钠三水合物缓冲液，用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述

Sodium citrate tribasic dihydrate

Synonyms: Citric acid trisodium salt dihydrate or Trisodium citrate dihydrate
C6H5Na3O7 • 2H2O
HOC(COONa)(CH2COONa)2 • 2H2O
Mr 294.10
CAS Number [6132-04-3]
EC Number 200-675-3
Merck 14,8602
Beilstein Registry Number 6104939
MDL Number MFCD00150031
Purity ≥ 99.0%

Useful pH range of a Sodium citrate tribasic dihydrate buffer: 2.2 – 6.5

Titrate HR2-571 to the desired pH using HCl
HR2-571 Measured pH Range: 8.1 – 9.4 at 25°C
HR2-571 Measured Conductivity Range: 60.0 – 69.8 mS/cm at 25°C
HR2-571 Measured Refractive Index Range: 1.37345 – 1.37370 at 20°C

HR2-735 is titrated to pH 5.6 using HCl
HR2-735 Measured Conductivity Range: 67.4 – 78.5 mS/cm at 25°C
HR2-735 Measured Refractive Index Range: 1.37400 – 1.37502 at 20°C

别名：柠檬酸三钠盐二水合物或柠檬酸三钠二水合物
C6H5Na3O7 • 2H2O
HOC(COONa)(CH2COONa)2 • 2H2O
294.10 先生
CAS 编号 [6132-04-3]
欧盟编号 200-675-3
默克 14,8602
贝尔斯坦注册号 6104939
MDL 编号 MFCD00150031
纯度≥99.0%

柠檬酸钠三水合物缓冲液的有用 pH 范围：2.2 – 6.5

使用 HCl 将 HR2-571 滴定至所需的 pH 值
HR2-571 测量的 pH 范围：25°C 时 8.1 – 9.4
HR2-571 测量的电导率范围：25°C 时为 60.0 – 69.8 mS/cm
HR2-571 测量的折射率范围：20°C 时为 1.37345 – 1.37370

使用 HCl 将 HR2-735 滴定至 pH 5.6
HR2-735 测得的电导率范围：25°C 时为 67.4 – 78.5 mS/cm
HR2-735 测量的折射率范围：20°C 时为 1.37400 – 1.37502

Al  ≤0.0005%
As ≤0.00001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.005%
Cd ≤0.0005%
Cl  ≤0.001%
Co ≤0.0005%
Cr ≤0.0005%
Cu ≤0.0005%
Fe ≤0.0005%
K   ≤0.01%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
NH4+ ≤0.001%
Ni  ≤0.0005%
Pb ≤0.0005%
SO4 ≤0.005%
Zn ≤0.0005%

Sodium citrate tribasic dihydrate

相关项目

  • Hydrochloric acid
  • StockOptions Sodium Citrate Buffer Kit
  • Individual StockOptions Sodium Citrate Reagents
  • 盐酸
    StockOptions 柠檬酸钠缓冲液套件
    个别股票期权柠檬酸钠试剂

参考

1. Crystallization and preliminary X-ray diffraction studies on ADP-ribosylation factor 1. Greasley, S; Jhoti, H; Fensome, AC; Cockcroft, S; Thomas, GM; Bax, B. J Mol Biol 244, 651- 653, 1994.

1. ADP-核糖基化因子的结晶和初步 X 射线衍射研究 1. Greasley, S; 乔蒂，H； 芬索姆，AC； 考克罗夫特，S； 托马斯，总经理； Bax, B. J Mol Biol 244, 651-653, 1994。

日本同仁化学Hampton蛋白结晶试剂盒TMAO/HR2-857| DOJINDO

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上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Cryoprotectants > TMAO

TMAO

Hampton蛋白结晶试剂盒TMAO/HR2-857

CAT NO

HR2-857

NAME

4.0 M TMAO

DESCRIPTION

1 mL

PRICE

$26.00

支持材料

Hampton蛋白结晶试剂盒TMAO/HR2-857 HR2-857 4.0 M TMAO SDS

应用

  • Water soluble cryoprotectant
  • 水溶性冷冻保护剂

特征

  • A very versatile cryoprotectant for macromolecular crystals
  • Protein stabilizer
  • TMAO can be a viable alternative when conventional cryoprotectants fail
  • Low viscosity and low surface tension
  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 一种非常通用的大分子晶体冷冻保护剂
    蛋白质稳定剂
    当传统冷冻保护剂失效时，TMAO 可能是一种可行的替代方案
    低粘度和低表面张力
    无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Trimethylamine N-oxide dihydrate (TMAO)

Formulated from TMAO dihydrate in Type 1 lab water (deionized) and sterile filtered (0.22 micron)

Synonyms: TMANO, TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
Mr 111.14
CAS Number [62637-93-8]
EC Number 214-675-6
Beilstein Registry Number 4(4)144
Merck 14,9711
RTECS YH2850000
Purity ≥ 98.0%
Measured pH range: 9.2 – 10.6 at 25°C
Measured Conductivity Range: 5.6 – 96.6 µS/cm at 25°C
Measured Refractive Index Range: 1.37660 – 1.37804 at 20°C

When using TMAO as a cryoprotectant, try soaking the crystals in 4.0 M TMAO for 10 seconds and then cryo cool directly in the cryo stream.

由 1 型实验室用水（去离子）和无菌过滤（0.22 微米）中的 TMAO 二水合物配制而成

同义词：TMANO、TMAO
(CH3)3NO•2H2O
C3H9NO•2H2O
111.14 先生
CAS 编号 [62637-93-8]
欧盟编号 214-675-6
贝尔斯坦注册号 4(4)144
默克 14,9711
RTECS YH2850000
纯度≥98.0%
测得的 pH 值范围：25°C 时 9.2 – 10.6
测得的电导率范围：25°C 时 5.6 – 96.6 µS/cm
测得的折射率范围：20°C 时为 1.37660 – 1.37804

当使用 TMAO 作为冷冻保护剂时，尝试将晶体浸泡在 4.0 M TMAO 中 10 秒，然后直接在冷冻流中冷冻冷却。

相关项目

  • CryoPro

参考

1. Trimethylamine N-oxide as a versatile cryoprotective agent in macromolecular crystallography. Christoph Mueller-Dieckmann, Brice Kauffmann and Manfred S. Weiss. J. Appl. Cryst. (2011). 44, 433–436 doi:10.1107/S0021889811000045.

1. 三甲胺 N-氧化物作为大分子晶体学中的多功能冷冻保护剂。 Christoph Mueller-Dieckmann、Brice Kauffmann 和 Manfred S. Weiss。 J.应用。 水晶。 （2011）。 44, 433–436 doi:10.1107/S0021889811000045。

日本同仁化学Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Tris Buffer

Tris Buffer

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725

CAT NO

HR2-589

NAME

1.0 M Tris

DESCRIPTION

100 mL

PRICE

$60.00

CAT NO

HR2-725

NAME

1.0 M Tris pH 8.5

DESCRIPTION

100 mL

PRICE

$60.00

应用

  • Crystallization grade Tris for formulating screens or for optimization
  • 结晶级 Tris 用于配制筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Tris

Synonyms: Trizma base or 2-Amino-2-(hydroxymethyl)-1,3-propanediol or THAM or Tris base or Tris(hydroxymethyl)aminomethane or Trometamol
C4H11NO3
NH2C(CH2OH)3
Mr 121.14
CAS Number [77-86-1]
EC Number 201-064-4
Beilstein Registry Number 741883
Merck 14,9772
RTECS TY2900000
MDL Number MFCD00004679
PubChem Substance ID 24889854
Purity 99.8 – 100.1%
pKa = 8.1 at 25°C

Useful pH range of Tris: 7.0 – 9.0

Titrate HR2-589 to the desired pH using HCl
HR2-589 Measured pH Range: 10.9 – 11.1 at 25°C
HR2-589 Measured Conductivity Range: 193.3 – 236.7 µS/cm at 25°C
HR2-589 Measured Refractive Index: 1.34991 – 1.35058 at 20°C

HR2-725 is titrated to pH 8.5 using HCl
HR2-725 Measured Conductivity Range: 18.1 – 22.7 mS/cm at 25°C
HR2-725 Measured Refractive Index: 1.35310 – 1.35369 at 20°C

同义词： Trizma 碱或 2-Amino-2-(羟甲基)-1,3-丙二醇或 THAM 或 Tris 碱或 Tris(羟甲基)氨基甲烷或氨丁三醇
C4H11NO3
NH2C(CH2OH)3
121.14 先生
CAS 编号 [77-86-1]
欧盟编号 201-064-4
贝尔斯坦注册号 741883
默克 14,9772
RTECS TY2900000
MDL 编号 MFCD00004679
PubChem 物质 ID 24889854
纯度 99.8 – 100.1%
pKa = 8.1 在 25°C

Tris 的有用 pH 值范围：7.0 – 9.0

使用 HCl 将 HR2-589 滴定至所需的 pH 值
HR2-589 测得的 pH 范围：25°C 时为 10.9 – 11.1
HR2-589 测量的电导率范围：25°C 时为 193.3 – 236.7 µS/cm
HR2-589 实测折射率：20°C 时为 1.34991 – 1.35058

使用 HCl 将 HR2-725 滴定至 pH 8.5
HR2-725 测量的电导率范围：25°C 时为 18.1 – 22.7 mS/cm
HR2-725 实测折射率：20°C 时为 1.35310 – 1.35369

Insoluble Matter 0.005% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 5 ppm max
As  ≤ 5 ppm max
Ca  ≤ 5 ppm max
Cu  ≤ 5 ppm max
Fe  ≤ 5 ppm max
Pb  ≤ 5 ppm max
Mg ≤ 5 ppm max

Trizma is a registered trademark of Sigma-Aldrich Co.

支持材料

Hampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-589 Tris SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 HR2-725 Tris pH 8.5 SDSHampton蛋白结晶试剂盒Tris Buffer/HR2-589/HR2-725 Titration Table

相关项目

  • Hydrochloric acid
  • StockOptions Tris Buffer Kit
  • Individual StockOptions Tris Reagents
  • 盐酸
    StockOptions Tris 缓冲套件
    个人股票期权 Tris 试剂

参考

1. Purification and crystallization of Pseudomonas aeruginosa chloramphenicol acetyltransferase. Tian, Y; Beaman, TW; Roderick, SL; Proteins 28, 298- 300, 1997.

1. 铜绿假单胞菌氯霉素乙酰转移酶的纯化与结晶。 田，Y； 比曼，台湾； 罗德里克，SL； 蛋白质 28, 298-300, 1997。

日本同仁化学Hampton蛋白结晶试剂盒NDSB-195/HR2-703| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-195

NDSB-195

Hampton蛋白结晶试剂盒NDSB-195/HR2-703

CAT NO

HR2-703

NAME

NDSB-195

DESCRIPTION

5 grams

PRICE

$57.00

支持材料

Hampton蛋白结晶试剂盒NDSB-195/HR2-703 HR2-703 NDSB-195 User GuideHampton蛋白结晶试剂盒NDSB-195/HR2-703 HR2-703 NDSB-195 SDS

应用

  • Crystallization grade NDSB-195 for formulating screens or for optimization
  • 结晶级 NDSB-195 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

The non detergent sulfobetaines are zwitterionic over a wide pH range, easily removed by dialysis and do not absorb significantly in the near UV range.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-195
Synonyms: Dimethylethylammonium propane sulfonate or Non-detergent sulfobetaine
C7H17NO3S
Mr 195.30
Purity: ≥95% by TLC
Hygroscopic
Store at room temperature (+20 degrees Celsius)

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包含体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

非去污剂磺基甜菜碱在很宽的 pH 值范围内是两性离子的，很容易通过透析去除，并且在近紫外范围内不会显着吸收。

蛋白质样品中典型的有用 NDSB 浓度为 0.5-1.0 M。

NDSB-195
别名：二甲基乙基丙烷磺酸铵或非洗涤剂磺基甜菜碱
C7H17NO3S
195.30 先生
纯度：TLC ≥95%
吸湿性
常温（+20℃）保存

HR2-703

参考

1. Goldberg, M.E., et al. 1995. Folding & Design 1, 21.

2. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

3. Vuillard, L., et al. 1995. Biochem. J. 305, 337.

4. Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

日本同仁化学Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > TRIS hydrochloride Buffer

TRIS hydrochloride Buffer

Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727

CAT NO

HR2-579

NAME

1.0 M TRIS hydrochloride

DESCRIPTION

100 mL

PRICE

$63.00

CAT NO

HR2-727

NAME

1.0 M TRIS hydrochloride pH 8.5

DESCRIPTION

100 mL

PRICE

$63.00

支持材料

Hampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 HR2-579 TRIS hydrochloride SDSHampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 HR2-727 TRIS hydrochloride pH 8.5 SDSHampton蛋白结晶试剂盒TRIS hydrochloride Buffer/HR2-579/HR2-727 Titration Table

应用

  • Crystallization grade TRIS hydrochloride buffer for formulating screens or for optimization
  • 用于配制筛选或优化的结晶级 TRIS 盐酸盐缓冲液

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述TRIS hydrochloride

Synonyms: TRIS HCl or Trizma hydrochloride or Tris(hydroxymethyl)aminomethane hydrochloride
NH2C(CH2OH)3 • HCl
C4H11NO3 • HCl
Mr 157.60
[1185-53-1]
EC Number 214-684-5
Beilstein Registry Number 3675235
MDL Number MFCD00012590
PubChem Substance ID 24900398
Purity ≥ 99.0%

Titrate HR2-579 to the desired pH using NaOH
HR2-579 Measured pH Range: 3.9 – 4.0 at 25°C
HR2-579 Measured Conductivity Range: 52.3 – 53.7 mS/cm at 25°C
HR2-579 Measured Refractive Index Range: 1.35840 – 1.35891 at 20°C

HR2-727 has been titrated to pH 8.5 using NaOH
HR2-727 Measured Conductivity Range: 59.8 – 61.7 mS at 25°C
HR2-727 Measured Refractive Index Range: 1.35938 – 1.36019 at 20°C

Use StockOptions Tris Hydrochloride kit (HR2-237) for a set of 21 Tris hydrochloride reagents titrated to pH 7.0 to 9.0 in 0.1 pH increments.

同义词：TRIS HCl 或 Trizma 盐酸盐或 Tris(羟甲基)氨基甲烷盐酸盐
NH2C(CH2OH)3 • HCl
C4H11NO3 • HCl
157.60 先生
[1185-53-1]
欧盟编号 214-684-5
贝尔斯坦注册号 3675235
MDL 编号 MFCD00012590
PubChem 物质 ID 24900398
纯度≥99.0%

使用 NaOH 将 HR2-579 滴定至所需的 pH 值
HR2-579 测量的 pH 范围：25°C 时 3.9 – 4.0
HR2-579 测量的电导率范围：25°C 时为 52.3 – 53.7 mS/cm
HR2-579 测量的折射率范围：20°C 时为 1.35840 – 1.35891

HR2-727 已使用 NaOH 滴定至 pH 8.5
HR2-727 测得的电导率范围：25°C 时为 59.8 – 61.7 mS
HR2-727 测量的折射率范围：20°C 时为 1.35938 – 1.36019

将 StockOptions Tris 盐酸试剂盒 (HR2-237) 用于一套 21 种 Tris 盐酸试剂，以 0.1 的 pH 增量滴定至 pH 7.0 至 9.0。

Al  ≤0.0005%
As ≤0.0001%
Ba ≤0.0005%
Bi  ≤0.0005%
Ca ≤0.001%
Cd ≤0.0005%
Co ≤0.0005%
Cr  ≤0.0005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.005%
Li   ≤0.0005%
Mg ≤0.0005%
Mn ≤0.0005%
Mo ≤0.0005%
Na ≤0.005%
Ni  ≤0.0005%
Pb  ≤0.0005%
SO4 ≤0.005%
Sr  ≤0.0005%
Zn  ≤0.0005%

TRIS hydrchoride

相关项目

  • Sodium hydroxide
  • StockOptions Tris Hydrochloride Buffer Kit
  • Individual StockOptions Tris Hydrochloride Reagents
  • 氢氧化钠
    StockOptions Tris 盐酸缓冲液试剂盒
    个别股票期权 Tris 盐酸盐试剂

日本同仁化学Hampton蛋白结晶试剂盒NDSB-201/HR2-701| DOJINDO

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Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-201

NDSB-201

Hampton蛋白结晶试剂盒NDSB-201/HR2-701

CAT NO

HR2-701

NAME

NDSB-201

DESCRIPTION

5 grams

PRICE

$43.00

支持材料

Hampton蛋白结晶试剂盒NDSB-201/HR2-701 HR2-701 NDSB-201 User GuideHampton蛋白结晶试剂盒NDSB-201/HR2-701 HR2-701 NDSB-201 SDS

应用

  • Crystallization grade NDSB-201 for formulating screens or for optimization
  • 结晶级 NDSB-201 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

在非变性浓度下有效溶解蛋白质
可用于防止蛋白质聚集
非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
用于溶解性和稳定性的蛋白质特定添加剂

NDSB-201
Synonyms: 3-(1-Pyridino)-1-propane sulfonate
C8H11NO3S
Mr 201.26
CAS Number [15471-17-7]
EC Number 239-491-3
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

参考

1. Vuillard, L., et al, J. Biochem., 305, 337 (1995).

2. Goldberg, M.E., et al, Folding and Design, 1, 21 (1996).

3. Vuillard et al. J.Cryst Growth (1996) vol 168 pp 150-154.

日本同仁化学Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Hydrochloric acid

Hydrochloric acid

Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581

CAT NO

HR2-581

NAME

1.0 M Hydrochloric acid

DESCRIPTION

100 mL

PRICE

$38.00

支持材料

Hampton蛋白结晶试剂盒Hydrochloric acid/HR2-581 HR2-581 Hydrochloric acid SDS

应用

  • Crystallization grade Hydrochloric acid for formulating screens or for optimization
  • 结晶级盐酸，用于筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Hydrochloric acid

Synonyms: None
HCl
Mr 36.46
CAS Number [7647-01-0]
EC Number 231-595-7
Beilstein Registry Number 1098214
Merck 14,4794
RTECS MW4025000
MDL Number MFCD00011324
PubChem Substance ID 329754537
UN Number (RIDADR) UN 1789 8/PG 2

Measured Conductivity Range: 320.4 – 378.2 mS/cm at 25°C

The 1.0 M Hydrochloric acid is formulated using pyrogen free (<0.03 Endotoxin (EU/ml)) water. The final 1.0 M Hydrochloric acid is not certified pyrogen free.

同义词：无
盐酸
36.46 先生
CAS 编号 [7647-01-0]
欧盟编号 231-595-7
贝尔斯坦注册号 1098214
默克 14,4794
RTECS MW4025000
MDL 编号 MFCD00011324
PubChem 物质 ID 329754537
联合国编号 (RIDADR) UN 1789 8/PG 2

测得的电导率范围：25°C 时 320.4 – 378.2 mS/cm

使用无热原 (<0.03 内毒素 (EU/ml)) 的水配制 1.0 M 盐酸。 最后的 1.0 M 盐酸未经过认证无热原。

Ag ≤0.000001%
Al  ≤0.000005%
As ≤0.0000005%
Ba ≤0.000002%
Bi  ≤0.00001%
Br  ≤0.005%
Ca ≤0.00005%
Cd ≤0.000001%
Co ≤0.000001%
Cr ≤0.000002%
Cu ≤0.000002%
Fe ≤0.00002%
Hg ≤0.00001%
K   ≤0.00001%
Li   ≤0.000001%
Mg ≤0.00001%
Mn ≤0.000001%
Mo ≤0.000002%
Na ≤0.00005%
NH4+ ≤0.0001%
Ni   ≤0.000002%
PO4 ≤0.0005%
Pb   ≤0.000002%
SO4 ≤0.0001%
SO3 ≤0.0001%
Sr   ≤0.000001%
TI   ≤0.000005%
Zn   ≤0.000005%

Hydrochloric acid相关项目

  • BIS-TRIS Buffer
  • HEPES sodium Buffer
  • Imidazole Buffer
  • Sodium acetate trihydrate Buffer
  • Sodium cacodylate trihydrate Buffer
  • Sodium citrate tribasic dihydrate Buffer
  • Tris Buffer
  • BIS-TRIS 缓冲器
    HEPES钠缓冲液
    咪唑缓冲液
    三水乙酸钠缓冲液
    二甲胂酸钠三水合物缓冲液
    柠檬酸钠二水合物缓冲液
    Tris 缓冲区

日本同仁化学Hampton蛋白结晶试剂盒NDSB-211/HR2-793| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-211

NDSB-211

Hampton蛋白结晶试剂盒NDSB-211/HR2-793

CAT NO

HR2-793

NAME

NDSB-211

DESCRIPTION

5 grams

PRICE

$127.00

应用

  • Crystallization grade NDSB-211 for formulating screens or for optimization
  • 结晶级 NDSB-211 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-211
Synonyms: Dimethyl(2-hydroxyethyl)ammonium propane sulfonate or Non detergent sulfobetaine 211
C7H17NO4S
Mr 211.30
Purity: ≥99%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

支持材料HR2-793

Hampton蛋白结晶试剂盒NDSB-211/HR2-793 HR2-793 NDSB-211 User GuideHampton蛋白结晶试剂盒NDSB-211/HR2-793 HR2-793 NDSB-211 SDS

参考

1. Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.

2. Vuillard, L., et al. 1995. Biochem. J. 305-, 337.

日本同仁化学Hampton蛋白结晶试剂盒Sodium hydroxide/HR2-583| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Buffers > Sodium hydroxide

Sodium hydroxide

Hampton蛋白结晶试剂盒Sodium hydroxide/HR2-583

CAT NO

HR2-583

NAME

1.0 M Sodium hydroxide

DESCRIPTION

100 mL

PRICE

$38.00

支持材料

Hampton蛋白结晶试剂盒Sodium hydroxide/HR2-583 HR2-583 Sodium hydroxide SDS

应用

  • Crystallization grade Sodium hydroxide for formulating screens or for optimization
  • 结晶级氢氧化钠，用于筛选或优化

特征

  • Sterile filtered
  • Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25°C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/µL)
  • 无菌过滤
    在 1+ 型超纯水中配制：25°C 时电阻率为 18.2 兆欧厘米，总有机碳 < 5 ppb，无细菌（<1 细菌 (CFU/ml)），无热原（<0.03 内毒素 (EU/ml)） , 无 RNase (< 0.01 ng/mL) 和无 DNase (< 4 pg/µL)

描述Sodium hydroxide

Synonym: Caustic soda
NaOH
HNaO
Mr 40.00
CAS [1310-73-2]
EC Number 215-185-5
Merck 14,8627
MDL Number MFCD00003548
PubChem Substance ID 24899773
UN Number UN 1823 8/PG 2
RTECS WB4900000
Purity ≥ 98.0%

Al  ≤0.0005%
Ca ≤0.0005%
Cl  ≤0.005%
Cu ≤0.0005%
Fe  ≤0.0005%
K   ≤0.02%
Mg ≤0.0005%
P    ≤0.0005%
Pb  ≤0.001%
SO4 ≤0.05%
Zn  ≤0.0005%

Sodium hydroxide

相关项目

  • ADA Buffer
  • BICINE Buffer
  • HEPES Buffer
  • MES monohydrate Buffer
  • TRIS hydrochloride Buffer

日本同仁化学Hampton蛋白结晶试剂盒NDSB-221/HR2-791| DOJINDO

发表于由

上海金畔生物科技有限公司代理日本同仁化学 DOJINDO代理商全线产品，欢迎访问官网了解更多信息

上海金畔生物代理Hampton research品牌蛋白结晶试剂耗材工具等，我们将竭诚为您服务，欢迎访问Hampton research官网或者咨询我们获取更多相关Hampton research品牌产品信息。

Products > Optimize Reagents > Optimize – Solubilizing Agents (NDSB) > NDSB-221

NDSB-221

Hampton蛋白结晶试剂盒NDSB-221/HR2-791

CAT NO

HR2-791

NAME

NDSB-221

DESCRIPTION

5 grams

PRICE

$58.00

支持材料

Hampton蛋白结晶试剂盒NDSB-221/HR2-791 HR2-791 NDSB-221 User GuideHampton蛋白结晶试剂盒NDSB-221/HR2-791 HR2-791 NDSB-221 SDS

应用

  • Crystallization grade NDSB-221 for formulating screens or for optimization
  • 结晶级 NDSB-221 用于配制筛选或优化

特征

  • Efficiently solubilizes proteins at non-denaturing concentrations
  • Useful in preventing protein aggregation
  • Non detergent sulfobetaines (NDSB) are used for protein folding, renaturation and crystallization
  • Protein specific additive for solubility and stability
  • 在非变性浓度下有效溶解蛋白质
    可用于防止蛋白质聚集
    非洗涤剂磺基甜菜碱 (NDSB) 用于蛋白质折叠、复性和结晶
    用于溶解性和稳定性的蛋白质特定添加剂

描述

The NDSB are a group of zwitterionic compounds that can reduce aggregation and aid in refolding proteins found in inclusion bodies and bacterial expression systems.

Non detergent sulfobetaines have a sulfobetaine hydrophilic group and a short hydrophobic group that cannot aggregate to form micelles, therefore NDSB’s are not considered detergents. NDSB increase the extraction yield (up to 30%) of membrane, nuclear and cyto-skeletal associated proteins. The short hydrophobic groups combined with the charge neutralization of the sulfobetaine group results in higher yields of membrane proteins. NDSB have been used in refolding and renaturation of chemically and thermally denatured proteins.

NDSB 是一组两性离子化合物，可以减少聚集并帮助重新折叠包涵体和细菌表达系统中发现的蛋白质。

非洗涤剂磺基甜菜碱有一个磺基甜菜碱亲水基团和一个不能聚集形成胶束的短疏水基团，因此 NDSB 不被视为洗涤剂。 NDSB 可提高膜、核和细胞骨架相关蛋白的提取率（高达 30%）。 短疏水基团与磺基甜菜碱基团的电荷中和相结合，导致膜蛋白产量更高。 NDSB 已用于化学和热变性蛋白质的重折叠和复性。

Typical useful NDSB concentration in protein sample is 0.5-1.0 M.

NDSB-221
Synonyms: Non-detergent sulfobetaine 221 or 3-(1-Methylpiperidinium)-1-propane Sulfonate
C9H19NO3S
Mr 221.34
Purity: ≥99.0%
Hygroscopic
Store at room temperature (+20 degrees Celsius)

HR2-791参考

1. Goldberg, M.E., et al. 1996. Folding & Design 1, 21.

2. Protein crystallography with non detergent sulfobetaines. L. Vuillard et al. J Cryst Growth (1996) 168 150-15.

3. Vuillard L, Rabilloud T, Goldberg ME, Eur J Biochem 1998 256(1):128-135. (protein folding).